Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid
Knowledge of the impact of aquaculture chemotherapeutants on fish physiology is scarce. This is particularly relevant for peracetic acid (PAA), a widely used oxidative disinfectant in aquaculture. The chemical behaviour in water is well studied but knowledge about the physiological consequences for...
Published in: | Aquaculture |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2021
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Online Access: | https://orbit.dtu.dk/en/publications/0a1f248a-e638-42f3-843e-1264aa50cd00 https://doi.org/10.1016/j.aquaculture.2020.735830 https://backend.orbit.dtu.dk/ws/files/218268519/berat_1_s2.0_S0044848620317932_main.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/0a1f248a-e638-42f3-843e-1264aa50cd00 2024-06-23T07:51:22+00:00 Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid Lazado, Carlo C. Sveen, Lene R. Soleng, Malene Pedersen, Lars-Flemming Timmerhaus, Gerrit 2021 application/pdf https://orbit.dtu.dk/en/publications/0a1f248a-e638-42f3-843e-1264aa50cd00 https://doi.org/10.1016/j.aquaculture.2020.735830 https://backend.orbit.dtu.dk/ws/files/218268519/berat_1_s2.0_S0044848620317932_main.pdf eng eng https://orbit.dtu.dk/en/publications/0a1f248a-e638-42f3-843e-1264aa50cd00 info:eu-repo/semantics/openAccess Lazado , C C , Sveen , L R , Soleng , M , Pedersen , L-F & Timmerhaus , G 2021 , ' Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid ' , Aquaculture , vol. 531 , 735830 . https://doi.org/10.1016/j.aquaculture.2020.735830 Amoebic gill disease Crowding stress Hydrogen peroxide Mucosal health Oxidative stress Peracetic acid article 2021 ftdtupubl https://doi.org/10.1016/j.aquaculture.2020.735830 2024-06-11T14:55:44Z Knowledge of the impact of aquaculture chemotherapeutants on fish physiology is scarce. This is particularly relevant for peracetic acid (PAA), a widely used oxidative disinfectant in aquaculture. The chemical behaviour in water is well studied but knowledge about the physiological consequences for fish is limited. The present study investigated the transcriptomics, morphology, and physiology of Atlantic salmon (Salmo salar) responses to PAA and explored how crowding prior to exposure influenced these responses. Post-smolts were subjected to crowding by reducing the water volume thereby increasing the density for 1 h before they were exposed to 4.8 ppm PAA for 30 min. The exposed fish were allowed to recover for 2 weeks (w), with samplings carried out at 4 h and 2 w post-exposure (p.e.). There were four treatment groups in total: no crowding/control; no crowding/PAA; crowding/control; and crowding/PAA. The physiological changes were documented at the mucosal (i.e., skin and gills) and systemic (i.e., plasma) levels. The overall external welfare score was in good status in all experimental groups. The treatments did not dramatically affect the number of mucous cells in both the skin and the gills. Branchial histomorphology was in a fairly good condition, despite the increased occurrence of epithelial lifting in the crowded groups at 2 w p.e. The gill transcriptome was affected by crowding, PAA, and their combinations more than the skin, as manifested by the number of differentially expressed genes (DEG) in the former. In general, individual stimuli and their combinations elicited strong transcriptional responses in the gills at 4 h p.e. and a marked recovery was observed 2 w thereafter. Crowding altered the dynamics of transcriptional response to PAA especially at 4 h p.e. and the two mucosal tissues demonstrated a contrasting profile – a higher number of DEGs in the gills without crowding history, while higher skin DEGs were observed in the group subjected to crowding prior to exposure. Plasma metabolomics ... Article in Journal/Newspaper Atlantic salmon Salmo salar Technical University of Denmark: DTU Orbit Paa ENVELOPE(-53.483,-53.483,66.017,66.017) Aquaculture 531 735830 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Amoebic gill disease Crowding stress Hydrogen peroxide Mucosal health Oxidative stress Peracetic acid |
spellingShingle |
Amoebic gill disease Crowding stress Hydrogen peroxide Mucosal health Oxidative stress Peracetic acid Lazado, Carlo C. Sveen, Lene R. Soleng, Malene Pedersen, Lars-Flemming Timmerhaus, Gerrit Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid |
topic_facet |
Amoebic gill disease Crowding stress Hydrogen peroxide Mucosal health Oxidative stress Peracetic acid |
description |
Knowledge of the impact of aquaculture chemotherapeutants on fish physiology is scarce. This is particularly relevant for peracetic acid (PAA), a widely used oxidative disinfectant in aquaculture. The chemical behaviour in water is well studied but knowledge about the physiological consequences for fish is limited. The present study investigated the transcriptomics, morphology, and physiology of Atlantic salmon (Salmo salar) responses to PAA and explored how crowding prior to exposure influenced these responses. Post-smolts were subjected to crowding by reducing the water volume thereby increasing the density for 1 h before they were exposed to 4.8 ppm PAA for 30 min. The exposed fish were allowed to recover for 2 weeks (w), with samplings carried out at 4 h and 2 w post-exposure (p.e.). There were four treatment groups in total: no crowding/control; no crowding/PAA; crowding/control; and crowding/PAA. The physiological changes were documented at the mucosal (i.e., skin and gills) and systemic (i.e., plasma) levels. The overall external welfare score was in good status in all experimental groups. The treatments did not dramatically affect the number of mucous cells in both the skin and the gills. Branchial histomorphology was in a fairly good condition, despite the increased occurrence of epithelial lifting in the crowded groups at 2 w p.e. The gill transcriptome was affected by crowding, PAA, and their combinations more than the skin, as manifested by the number of differentially expressed genes (DEG) in the former. In general, individual stimuli and their combinations elicited strong transcriptional responses in the gills at 4 h p.e. and a marked recovery was observed 2 w thereafter. Crowding altered the dynamics of transcriptional response to PAA especially at 4 h p.e. and the two mucosal tissues demonstrated a contrasting profile – a higher number of DEGs in the gills without crowding history, while higher skin DEGs were observed in the group subjected to crowding prior to exposure. Plasma metabolomics ... |
format |
Article in Journal/Newspaper |
author |
Lazado, Carlo C. Sveen, Lene R. Soleng, Malene Pedersen, Lars-Flemming Timmerhaus, Gerrit |
author_facet |
Lazado, Carlo C. Sveen, Lene R. Soleng, Malene Pedersen, Lars-Flemming Timmerhaus, Gerrit |
author_sort |
Lazado, Carlo C. |
title |
Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid |
title_short |
Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid |
title_full |
Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid |
title_fullStr |
Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid |
title_full_unstemmed |
Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid |
title_sort |
crowding reshapes the mucosal but not the systemic response repertoires of atlantic salmon to peracetic acid |
publishDate |
2021 |
url |
https://orbit.dtu.dk/en/publications/0a1f248a-e638-42f3-843e-1264aa50cd00 https://doi.org/10.1016/j.aquaculture.2020.735830 https://backend.orbit.dtu.dk/ws/files/218268519/berat_1_s2.0_S0044848620317932_main.pdf |
long_lat |
ENVELOPE(-53.483,-53.483,66.017,66.017) |
geographic |
Paa |
geographic_facet |
Paa |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Lazado , C C , Sveen , L R , Soleng , M , Pedersen , L-F & Timmerhaus , G 2021 , ' Crowding reshapes the mucosal but not the systemic response repertoires of Atlantic salmon to peracetic acid ' , Aquaculture , vol. 531 , 735830 . https://doi.org/10.1016/j.aquaculture.2020.735830 |
op_relation |
https://orbit.dtu.dk/en/publications/0a1f248a-e638-42f3-843e-1264aa50cd00 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2020.735830 |
container_title |
Aquaculture |
container_volume |
531 |
container_start_page |
735830 |
_version_ |
1802642448643522560 |