Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae
Thyroid hormones (THs) are key regulators of growth, development, and metabolism in vertebrates and influence early life development of fish. TH is produced in the thyroid gland (or thyroid follicles) mainly as T4 (thyroxine), which is metabolized to T3 (3,5,3'-triiodothyronine) and T2 (3,5-dii...
Published in: | General and Comparative Endocrinology |
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2018
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ftdtupubl:oai:pure.atira.dk:publications/08916c00-9e55-4f86-a046-a160888bf9b6 2024-04-28T07:55:07+00:00 Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae Politis, Sebastian Nikitas Servili, A. Mazurais, D. Zambonino-Infante, J.-L. Miest, J. J. Tomkiewicz, Jonna Butts, I. A. E. 2018 application/pdf https://orbit.dtu.dk/en/publications/08916c00-9e55-4f86-a046-a160888bf9b6 https://doi.org/10.1016/j.ygcen.2017.11.003 https://backend.orbit.dtu.dk/ws/files/139327655/Postprint.pdf eng eng https://orbit.dtu.dk/en/publications/08916c00-9e55-4f86-a046-a160888bf9b6 info:eu-repo/semantics/openAccess Politis , S N , Servili , A , Mazurais , D , Zambonino-Infante , J-L , Miest , J J , Tomkiewicz , J & Butts , I A E 2018 , ' Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae ' , General and Comparative Endocrinology , vol. 259 , pp. 54-65 . https://doi.org/10.1016/j.ygcen.2017.11.003 Deiodinases Early life history Fish Larvae Thyroid hormone receptors article 2018 ftdtupubl https://doi.org/10.1016/j.ygcen.2017.11.003 2024-04-03T15:29:59Z Thyroid hormones (THs) are key regulators of growth, development, and metabolism in vertebrates and influence early life development of fish. TH is produced in the thyroid gland (or thyroid follicles) mainly as T4 (thyroxine), which is metabolized to T3 (3,5,3'-triiodothyronine) and T2 (3,5-diiodothyronine) by deiodinase (DIO) enzymes in peripheral tissues. The action of these hormones is mostly exerted by binding to a specific nuclear thyroid hormone receptor (THR). In this study, we i) cloned and characterized thr sequences, ii) investigated the expression pattern of the different subtypes of thrs and dios, and iii) studied how temperature affects the expression of those genes in artificially produced early life history stages of European eel (Anguilla anguilla), reared in different thermal regimes (16, 18, 20 and 22°C) from hatch until first-feeding. We identified 2 subtypes of thr (thrα and thrβ) with 2 isoforms each (thrαA, thrαB, thrβA, thrβB) and 3 subtypes of deiodinases (dio1, dio2, dio3). All thr genes identified showed high similarity to the closely related Japanese eel (Anguilla japonica). We found that all genes investigated in this study were affected by larval age (in real time or at specific developmental stages), temperature, and/or their interaction. More specifically, the warmer the temperature the earlier the expression response of a specific target gene. In real time, the expression profiles appeared very similar and only shifted with temperature. In developmental time, gene expression of all genes differed across selected developmental stages, such as at hatch, during teeth formation or at first-feeding. Thus, we demonstrate that the expression of thrs and dios show sensitivity to temperature and are involved in and during early life development of European eel. Article in Journal/Newspaper Anguilla anguilla Technical University of Denmark: DTU Orbit General and Comparative Endocrinology 259 54 65 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Deiodinases Early life history Fish Larvae Thyroid hormone receptors |
spellingShingle |
Deiodinases Early life history Fish Larvae Thyroid hormone receptors Politis, Sebastian Nikitas Servili, A. Mazurais, D. Zambonino-Infante, J.-L. Miest, J. J. Tomkiewicz, Jonna Butts, I. A. E. Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae |
topic_facet |
Deiodinases Early life history Fish Larvae Thyroid hormone receptors |
description |
Thyroid hormones (THs) are key regulators of growth, development, and metabolism in vertebrates and influence early life development of fish. TH is produced in the thyroid gland (or thyroid follicles) mainly as T4 (thyroxine), which is metabolized to T3 (3,5,3'-triiodothyronine) and T2 (3,5-diiodothyronine) by deiodinase (DIO) enzymes in peripheral tissues. The action of these hormones is mostly exerted by binding to a specific nuclear thyroid hormone receptor (THR). In this study, we i) cloned and characterized thr sequences, ii) investigated the expression pattern of the different subtypes of thrs and dios, and iii) studied how temperature affects the expression of those genes in artificially produced early life history stages of European eel (Anguilla anguilla), reared in different thermal regimes (16, 18, 20 and 22°C) from hatch until first-feeding. We identified 2 subtypes of thr (thrα and thrβ) with 2 isoforms each (thrαA, thrαB, thrβA, thrβB) and 3 subtypes of deiodinases (dio1, dio2, dio3). All thr genes identified showed high similarity to the closely related Japanese eel (Anguilla japonica). We found that all genes investigated in this study were affected by larval age (in real time or at specific developmental stages), temperature, and/or their interaction. More specifically, the warmer the temperature the earlier the expression response of a specific target gene. In real time, the expression profiles appeared very similar and only shifted with temperature. In developmental time, gene expression of all genes differed across selected developmental stages, such as at hatch, during teeth formation or at first-feeding. Thus, we demonstrate that the expression of thrs and dios show sensitivity to temperature and are involved in and during early life development of European eel. |
format |
Article in Journal/Newspaper |
author |
Politis, Sebastian Nikitas Servili, A. Mazurais, D. Zambonino-Infante, J.-L. Miest, J. J. Tomkiewicz, Jonna Butts, I. A. E. |
author_facet |
Politis, Sebastian Nikitas Servili, A. Mazurais, D. Zambonino-Infante, J.-L. Miest, J. J. Tomkiewicz, Jonna Butts, I. A. E. |
author_sort |
Politis, Sebastian Nikitas |
title |
Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae |
title_short |
Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae |
title_full |
Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae |
title_fullStr |
Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae |
title_full_unstemmed |
Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae |
title_sort |
temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of european eel (anguilla anguilla) larvae |
publishDate |
2018 |
url |
https://orbit.dtu.dk/en/publications/08916c00-9e55-4f86-a046-a160888bf9b6 https://doi.org/10.1016/j.ygcen.2017.11.003 https://backend.orbit.dtu.dk/ws/files/139327655/Postprint.pdf |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Politis , S N , Servili , A , Mazurais , D , Zambonino-Infante , J-L , Miest , J J , Tomkiewicz , J & Butts , I A E 2018 , ' Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae ' , General and Comparative Endocrinology , vol. 259 , pp. 54-65 . https://doi.org/10.1016/j.ygcen.2017.11.003 |
op_relation |
https://orbit.dtu.dk/en/publications/08916c00-9e55-4f86-a046-a160888bf9b6 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.ygcen.2017.11.003 |
container_title |
General and Comparative Endocrinology |
container_volume |
259 |
container_start_page |
54 |
op_container_end_page |
65 |
_version_ |
1797578551536386048 |