Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.)
European North Atlantic ranavirus (ENARV, Iridoviridae), is a ranavirus species recently isolated from lumpfish ( Cyclopterus lumpus , L.), which are used as cleaner fish in Atlantic salmon ( Salmo salar ) farming in Northern Europe. This study aimed to investigate (1) the virulence of ENARV isolate...
Published in: | Journal of Fish Diseases |
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Language: | English |
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2022
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Online Access: | https://orbit.dtu.dk/en/publications/04084e60-ef67-4041-986a-b1b99d06cca5 https://doi.org/10.1111/jfd.13696 https://backend.orbit.dtu.dk/ws/files/290539960/Journal_of_Fish_Diseases_2022_Scholz_Experimental_infection_trials_with_European_North_Atlantic_ranavirus_.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/04084e60-ef67-4041-986a-b1b99d06cca5 2023-05-15T15:30:24+02:00 Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) Scholz, Felix Vendramin, Niccolò Olesen, Niels Jørgen Cuenca, Argelia Moesgaard Iburg, Tine Mirimin, Luca O'Connor, Ian Ruane, Neil M. Rodger, Hamish D. MacCarthy, Eugene 2022 application/pdf https://orbit.dtu.dk/en/publications/04084e60-ef67-4041-986a-b1b99d06cca5 https://doi.org/10.1111/jfd.13696 https://backend.orbit.dtu.dk/ws/files/290539960/Journal_of_Fish_Diseases_2022_Scholz_Experimental_infection_trials_with_European_North_Atlantic_ranavirus_.pdf eng eng info:eu-repo/semantics/openAccess Scholz , F , Vendramin , N , Olesen , N J , Cuenca , A , Moesgaard Iburg , T , Mirimin , L , O'Connor , I , Ruane , N M , Rodger , H D & MacCarthy , E 2022 , ' Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) ' , Journal of Fish Diseases , vol. 45 , no. 11 , pp. 1745-1756 . https://doi.org/10.1111/jfd.13696 Atlantic salmon (salmo salar) Cleaner fish Cohabitation challenge trial European north atlantic ranavirus (ENARV) Lumpfish (cyclopterus lumpus) Virulence article 2022 ftdtupubl https://doi.org/10.1111/jfd.13696 2023-01-12T00:17:29Z European North Atlantic ranavirus (ENARV, Iridoviridae), is a ranavirus species recently isolated from lumpfish ( Cyclopterus lumpus , L.), which are used as cleaner fish in Atlantic salmon ( Salmo salar ) farming in Northern Europe. This study aimed to investigate (1) the virulence of ENARV isolates from Ireland, Iceland and the Faroe Islands to lumpfish; (2) horizontal transmission between lumpfish; and (3) virulence to Atlantic salmon parr. Lumpfish were challenged in a cohabitation model using intraperitoneally (IP) injected shedders, and naïve cohabitants. IP challenge with isolates from Iceland (1.9 × 10 7 TCID 50 ml -1 ) and the Faroe Islands (5.9 × 10 7 TCID 50 ml -1 ) reduced survival in lumpfish, associated with consistent pathological changes. IP challenge with the Irish strain (8.6 × 10 5 TCID 50 ml -1 ) did not significantly reduce survival in lumpfish, but the lower challenge titre complicated interpretation. Horizontal transmission occurred in all strains tested, but no clinical impact was demonstrated in cohabitants. Salmon parr were challenged by IP injection with the Irish isolate, no virulence or virus replication were demonstrated. A ranavirus qPCR assay, previously validated for fish ranaviruses, was first used to detect ENARV in tissues of both in lumpfish and Atlantic salmon. This study provides the first data on the assessment of virulence of ENARV isolates to lumpfish and salmon, guidelines for the diagnosis of ENARV infection, and poses a basis for further investigations into virulence markers. Article in Journal/Newspaper Atlantic salmon Faroe Islands Iceland North Atlantic Salmo salar Technical University of Denmark: DTU Orbit Faroe Islands Journal of Fish Diseases 45 11 1745 1756 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Atlantic salmon (salmo salar) Cleaner fish Cohabitation challenge trial European north atlantic ranavirus (ENARV) Lumpfish (cyclopterus lumpus) Virulence |
spellingShingle |
Atlantic salmon (salmo salar) Cleaner fish Cohabitation challenge trial European north atlantic ranavirus (ENARV) Lumpfish (cyclopterus lumpus) Virulence Scholz, Felix Vendramin, Niccolò Olesen, Niels Jørgen Cuenca, Argelia Moesgaard Iburg, Tine Mirimin, Luca O'Connor, Ian Ruane, Neil M. Rodger, Hamish D. MacCarthy, Eugene Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) |
topic_facet |
Atlantic salmon (salmo salar) Cleaner fish Cohabitation challenge trial European north atlantic ranavirus (ENARV) Lumpfish (cyclopterus lumpus) Virulence |
description |
European North Atlantic ranavirus (ENARV, Iridoviridae), is a ranavirus species recently isolated from lumpfish ( Cyclopterus lumpus , L.), which are used as cleaner fish in Atlantic salmon ( Salmo salar ) farming in Northern Europe. This study aimed to investigate (1) the virulence of ENARV isolates from Ireland, Iceland and the Faroe Islands to lumpfish; (2) horizontal transmission between lumpfish; and (3) virulence to Atlantic salmon parr. Lumpfish were challenged in a cohabitation model using intraperitoneally (IP) injected shedders, and naïve cohabitants. IP challenge with isolates from Iceland (1.9 × 10 7 TCID 50 ml -1 ) and the Faroe Islands (5.9 × 10 7 TCID 50 ml -1 ) reduced survival in lumpfish, associated with consistent pathological changes. IP challenge with the Irish strain (8.6 × 10 5 TCID 50 ml -1 ) did not significantly reduce survival in lumpfish, but the lower challenge titre complicated interpretation. Horizontal transmission occurred in all strains tested, but no clinical impact was demonstrated in cohabitants. Salmon parr were challenged by IP injection with the Irish isolate, no virulence or virus replication were demonstrated. A ranavirus qPCR assay, previously validated for fish ranaviruses, was first used to detect ENARV in tissues of both in lumpfish and Atlantic salmon. This study provides the first data on the assessment of virulence of ENARV isolates to lumpfish and salmon, guidelines for the diagnosis of ENARV infection, and poses a basis for further investigations into virulence markers. |
format |
Article in Journal/Newspaper |
author |
Scholz, Felix Vendramin, Niccolò Olesen, Niels Jørgen Cuenca, Argelia Moesgaard Iburg, Tine Mirimin, Luca O'Connor, Ian Ruane, Neil M. Rodger, Hamish D. MacCarthy, Eugene |
author_facet |
Scholz, Felix Vendramin, Niccolò Olesen, Niels Jørgen Cuenca, Argelia Moesgaard Iburg, Tine Mirimin, Luca O'Connor, Ian Ruane, Neil M. Rodger, Hamish D. MacCarthy, Eugene |
author_sort |
Scholz, Felix |
title |
Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) |
title_short |
Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) |
title_full |
Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) |
title_fullStr |
Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) |
title_full_unstemmed |
Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) |
title_sort |
experimental infection trials with european north atlantic ranavirus ( iridoviridae ) isolated from lumpfish ( cyclopterus lumpus, l.) |
publishDate |
2022 |
url |
https://orbit.dtu.dk/en/publications/04084e60-ef67-4041-986a-b1b99d06cca5 https://doi.org/10.1111/jfd.13696 https://backend.orbit.dtu.dk/ws/files/290539960/Journal_of_Fish_Diseases_2022_Scholz_Experimental_infection_trials_with_European_North_Atlantic_ranavirus_.pdf |
geographic |
Faroe Islands |
geographic_facet |
Faroe Islands |
genre |
Atlantic salmon Faroe Islands Iceland North Atlantic Salmo salar |
genre_facet |
Atlantic salmon Faroe Islands Iceland North Atlantic Salmo salar |
op_source |
Scholz , F , Vendramin , N , Olesen , N J , Cuenca , A , Moesgaard Iburg , T , Mirimin , L , O'Connor , I , Ruane , N M , Rodger , H D & MacCarthy , E 2022 , ' Experimental infection trials with European North Atlantic ranavirus ( Iridoviridae ) isolated from lumpfish ( Cyclopterus lumpus, L.) ' , Journal of Fish Diseases , vol. 45 , no. 11 , pp. 1745-1756 . https://doi.org/10.1111/jfd.13696 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/jfd.13696 |
container_title |
Journal of Fish Diseases |
container_volume |
45 |
container_issue |
11 |
container_start_page |
1745 |
op_container_end_page |
1756 |
_version_ |
1766360846456324096 |