Uncovering Mechanisms for Repair and Protection in Cold Environments Through Studies of Cold Adapted Archaea
Methanococcoides burtonii is a cold-adapted archaeon isolated from permanently cold (1-2 deg C), methane saturated waters in Ace Lake, Antarctica. M. burtonii is a motile, flagellated microbe that uses methylated carbon compounds for growth (methylotrophy), such as methanol and trimethylamine. Altho...
Main Authors: | , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Published: |
2009
|
Subjects: | |
Online Access: | http://www.dtic.mil/docs/citations/ADA512653 http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=ADA512653 |
id |
ftdtic:ADA512653 |
---|---|
record_format |
openpolar |
spelling |
ftdtic:ADA512653 2023-05-15T14:02:50+02:00 Uncovering Mechanisms for Repair and Protection in Cold Environments Through Studies of Cold Adapted Archaea Cavicchioli, Ricardo Williams, Tim Pilak, Oliver NEW SOUTH WALES UNIV SYDNEY (AUSTRALIA) 2009-12-18 text/html http://www.dtic.mil/docs/citations/ADA512653 http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=ADA512653 en eng http://www.dtic.mil/docs/citations/ADA512653 Approved for public release; distribution is unlimited. DTIC Biochemistry Stress Physiology Microbiology Biological Oceanography *MICROORGANISMS *ANTARCTIC REGIONS *COLD TOLERANCE *ADAPTATION(PHYSIOLOGY) PROTECTION MOLECULAR BIOLOGY AUSTRALIA AQUATIC ORGANISMS LABELED SUBSTANCES GROWTH(PHYSIOLOGY) MASS SPECTROMETRY PHOSPHORYLATION LIQUID CHROMATOGRAPHY REPAIR STRESS(PHYSIOLOGY) LOW TEMPERATURE PROTEINS *COLD ADAPTATION *METHANOCOCCOIDES BURTONII ARCHAEA PROTEOMIC ANALYSIS PROTEIN FOLDING CHAPERONIN COMPLEX CPN60(CHAPERONIN) THERMAL ADAPTATION WESTERN BLOT ANALYSIS CELL GROWTH FOREIGN REPORTS Text 2009 ftdtic 2016-02-22T22:52:33Z Methanococcoides burtonii is a cold-adapted archaeon isolated from permanently cold (1-2 deg C), methane saturated waters in Ace Lake, Antarctica. M. burtonii is a motile, flagellated microbe that uses methylated carbon compounds for growth (methylotrophy), such as methanol and trimethylamine. Although adapted to the cold, M. burtonii is capable of growth at much higher temperatures, with the highest (= optimal) growth rate occurring at 23 deg C, and a maximum growth temperature of 28 deg C. We have employed two separate but integrated approaches for investigating the basis of cold adaptation in M. burtonii: targeted protein studies, and global proteomic analysis. Our first approach focuses on the study of the chaperonin (Cpn60) complex, which is important for the correct folding of proteins inside the cell. Our second approach aims to determine how protein expression changes in the entire cell at different growth temperatures, using isotope labeling of proteins and liquid chromatography-tandem mass spectrometry (LC/LC-MS/MS). Our research aims to provide fundamental knowledge about the molecular mechanisms of adaptation to growth at cold temperatures in archaea. This will enable us to identify molecular strategies that have evolved in order to cope with extreme cold, with the cell in a state of permanent cold stress. This is interesting in terms of both the limits of archaeal adaptation to low temperature extremes, and in considering whether archaeal mechanisms for cold adaptation may be extended to other systems. Text Antarc* Antarctic Antarctica Defense Technical Information Center: DTIC Technical Reports database Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Antarctic |
institution |
Open Polar |
collection |
Defense Technical Information Center: DTIC Technical Reports database |
op_collection_id |
ftdtic |
language |
English |
topic |
Biochemistry Stress Physiology Microbiology Biological Oceanography *MICROORGANISMS *ANTARCTIC REGIONS *COLD TOLERANCE *ADAPTATION(PHYSIOLOGY) PROTECTION MOLECULAR BIOLOGY AUSTRALIA AQUATIC ORGANISMS LABELED SUBSTANCES GROWTH(PHYSIOLOGY) MASS SPECTROMETRY PHOSPHORYLATION LIQUID CHROMATOGRAPHY REPAIR STRESS(PHYSIOLOGY) LOW TEMPERATURE PROTEINS *COLD ADAPTATION *METHANOCOCCOIDES BURTONII ARCHAEA PROTEOMIC ANALYSIS PROTEIN FOLDING CHAPERONIN COMPLEX CPN60(CHAPERONIN) THERMAL ADAPTATION WESTERN BLOT ANALYSIS CELL GROWTH FOREIGN REPORTS |
spellingShingle |
Biochemistry Stress Physiology Microbiology Biological Oceanography *MICROORGANISMS *ANTARCTIC REGIONS *COLD TOLERANCE *ADAPTATION(PHYSIOLOGY) PROTECTION MOLECULAR BIOLOGY AUSTRALIA AQUATIC ORGANISMS LABELED SUBSTANCES GROWTH(PHYSIOLOGY) MASS SPECTROMETRY PHOSPHORYLATION LIQUID CHROMATOGRAPHY REPAIR STRESS(PHYSIOLOGY) LOW TEMPERATURE PROTEINS *COLD ADAPTATION *METHANOCOCCOIDES BURTONII ARCHAEA PROTEOMIC ANALYSIS PROTEIN FOLDING CHAPERONIN COMPLEX CPN60(CHAPERONIN) THERMAL ADAPTATION WESTERN BLOT ANALYSIS CELL GROWTH FOREIGN REPORTS Cavicchioli, Ricardo Williams, Tim Pilak, Oliver Uncovering Mechanisms for Repair and Protection in Cold Environments Through Studies of Cold Adapted Archaea |
topic_facet |
Biochemistry Stress Physiology Microbiology Biological Oceanography *MICROORGANISMS *ANTARCTIC REGIONS *COLD TOLERANCE *ADAPTATION(PHYSIOLOGY) PROTECTION MOLECULAR BIOLOGY AUSTRALIA AQUATIC ORGANISMS LABELED SUBSTANCES GROWTH(PHYSIOLOGY) MASS SPECTROMETRY PHOSPHORYLATION LIQUID CHROMATOGRAPHY REPAIR STRESS(PHYSIOLOGY) LOW TEMPERATURE PROTEINS *COLD ADAPTATION *METHANOCOCCOIDES BURTONII ARCHAEA PROTEOMIC ANALYSIS PROTEIN FOLDING CHAPERONIN COMPLEX CPN60(CHAPERONIN) THERMAL ADAPTATION WESTERN BLOT ANALYSIS CELL GROWTH FOREIGN REPORTS |
description |
Methanococcoides burtonii is a cold-adapted archaeon isolated from permanently cold (1-2 deg C), methane saturated waters in Ace Lake, Antarctica. M. burtonii is a motile, flagellated microbe that uses methylated carbon compounds for growth (methylotrophy), such as methanol and trimethylamine. Although adapted to the cold, M. burtonii is capable of growth at much higher temperatures, with the highest (= optimal) growth rate occurring at 23 deg C, and a maximum growth temperature of 28 deg C. We have employed two separate but integrated approaches for investigating the basis of cold adaptation in M. burtonii: targeted protein studies, and global proteomic analysis. Our first approach focuses on the study of the chaperonin (Cpn60) complex, which is important for the correct folding of proteins inside the cell. Our second approach aims to determine how protein expression changes in the entire cell at different growth temperatures, using isotope labeling of proteins and liquid chromatography-tandem mass spectrometry (LC/LC-MS/MS). Our research aims to provide fundamental knowledge about the molecular mechanisms of adaptation to growth at cold temperatures in archaea. This will enable us to identify molecular strategies that have evolved in order to cope with extreme cold, with the cell in a state of permanent cold stress. This is interesting in terms of both the limits of archaeal adaptation to low temperature extremes, and in considering whether archaeal mechanisms for cold adaptation may be extended to other systems. |
author2 |
NEW SOUTH WALES UNIV SYDNEY (AUSTRALIA) |
format |
Text |
author |
Cavicchioli, Ricardo Williams, Tim Pilak, Oliver |
author_facet |
Cavicchioli, Ricardo Williams, Tim Pilak, Oliver |
author_sort |
Cavicchioli, Ricardo |
title |
Uncovering Mechanisms for Repair and Protection in Cold Environments Through Studies of Cold Adapted Archaea |
title_short |
Uncovering Mechanisms for Repair and Protection in Cold Environments Through Studies of Cold Adapted Archaea |
title_full |
Uncovering Mechanisms for Repair and Protection in Cold Environments Through Studies of Cold Adapted Archaea |
title_fullStr |
Uncovering Mechanisms for Repair and Protection in Cold Environments Through Studies of Cold Adapted Archaea |
title_full_unstemmed |
Uncovering Mechanisms for Repair and Protection in Cold Environments Through Studies of Cold Adapted Archaea |
title_sort |
uncovering mechanisms for repair and protection in cold environments through studies of cold adapted archaea |
publishDate |
2009 |
url |
http://www.dtic.mil/docs/citations/ADA512653 http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=ADA512653 |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Ace Lake Antarctic |
geographic_facet |
Ace Lake Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
DTIC |
op_relation |
http://www.dtic.mil/docs/citations/ADA512653 |
op_rights |
Approved for public release; distribution is unlimited. |
_version_ |
1766273248302989312 |