Comparative Analyses of Aryl Hydrocarbon Receptor Structure, Function, and Evolution in Marine Mammals
Marine mammals possess high body burdens of persistent organic pollutants, including PCBs and dioxin-like compounds (DLC). Chronic environmental or dietary exposure to these chemicals can disrupt the function of reproductive and immune systems in laboratory animals. The aryl hydrocarbon receptor (AH...
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ftdtic:ADA472638 2023-05-15T16:36:07+02:00 Comparative Analyses of Aryl Hydrocarbon Receptor Structure, Function, and Evolution in Marine Mammals Lapseritis, Joy M. MASSACHUSETTS INST OF TECH CAMBRIDGE JOINT PROGRAM IN APPLIED OCEAN SCIENCE AND ENGINEERING 2007-02 text/html http://www.dtic.mil/docs/citations/ADA472638 http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=ADA472638 en eng http://www.dtic.mil/docs/citations/ADA472638 Approved for public release; distribution is unlimited. DTIC Biochemistry Medicine and Medical Research *HORMONES *IN VITRO ANALYSIS *HYDROCARBONS *IMMUNITY *MARINE BIOLOGY *RECEPTOR SITES(PHYSIOLOGY) *EVOLUTION(BIOLOGY) *REPRODUCTION(PHYSIOLOGY) MAMMALS ORGANIC MATERIALS GENES POLLUTANTS RESPONSE(BIOLOGY) WHALES LABORATORY ANIMALS AQUATIC ANIMALS SENSE ORGANS CULTURES(BIOLOGY) NORTH ATLANTIC OCEAN DIET CELLS(BIOLOGY) GENETIC ENGINEERING STEROIDS EXPOSURE(PHYSIOLOGY) CLONES ARYL RADICALS CONTAMINANTS CHEMICALS THESES Text 2007 ftdtic 2016-02-22T12:00:27Z Marine mammals possess high body burdens of persistent organic pollutants, including PCBs and dioxin-like compounds (DLC). Chronic environmental or dietary exposure to these chemicals can disrupt the function of reproductive and immune systems in laboratory animals. The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor. mediating the expression of a suite of genes in response to exposure to DLC and structurally related chemicals. Species-specific differences in AHR structure can affect an organism's susceptibility to the effects of DLC. The structures and functions of several cetacean AHRs were investigated using In vitro molecular cloning and biochemical techniques. Using remote biopsy and molecular cloning methods, RNA was extracted from small integument samples from living North Atlantic right whales to identify the AHR cDNA sequence and other genes. Molecular and biochemical characteristics for North Atlantic right whale and humpback whale AHR cDNAs were determined using in vitro and cell culture methods. The properties of these AHRs were compared with those from other model and marine mammalian species using biochemical, phylogenetic. and homology modeling analyses. Additional studies are necessary to link exposure to environmental contaminants with potential reproductive effects in marine mammals, especially via interactions with steroid hormone receptor pathways. Text Humpback Whale North Atlantic North Atlantic right whale Defense Technical Information Center: DTIC Technical Reports database |
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Defense Technical Information Center: DTIC Technical Reports database |
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English |
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Biochemistry Medicine and Medical Research *HORMONES *IN VITRO ANALYSIS *HYDROCARBONS *IMMUNITY *MARINE BIOLOGY *RECEPTOR SITES(PHYSIOLOGY) *EVOLUTION(BIOLOGY) *REPRODUCTION(PHYSIOLOGY) MAMMALS ORGANIC MATERIALS GENES POLLUTANTS RESPONSE(BIOLOGY) WHALES LABORATORY ANIMALS AQUATIC ANIMALS SENSE ORGANS CULTURES(BIOLOGY) NORTH ATLANTIC OCEAN DIET CELLS(BIOLOGY) GENETIC ENGINEERING STEROIDS EXPOSURE(PHYSIOLOGY) CLONES ARYL RADICALS CONTAMINANTS CHEMICALS THESES |
spellingShingle |
Biochemistry Medicine and Medical Research *HORMONES *IN VITRO ANALYSIS *HYDROCARBONS *IMMUNITY *MARINE BIOLOGY *RECEPTOR SITES(PHYSIOLOGY) *EVOLUTION(BIOLOGY) *REPRODUCTION(PHYSIOLOGY) MAMMALS ORGANIC MATERIALS GENES POLLUTANTS RESPONSE(BIOLOGY) WHALES LABORATORY ANIMALS AQUATIC ANIMALS SENSE ORGANS CULTURES(BIOLOGY) NORTH ATLANTIC OCEAN DIET CELLS(BIOLOGY) GENETIC ENGINEERING STEROIDS EXPOSURE(PHYSIOLOGY) CLONES ARYL RADICALS CONTAMINANTS CHEMICALS THESES Lapseritis, Joy M. Comparative Analyses of Aryl Hydrocarbon Receptor Structure, Function, and Evolution in Marine Mammals |
topic_facet |
Biochemistry Medicine and Medical Research *HORMONES *IN VITRO ANALYSIS *HYDROCARBONS *IMMUNITY *MARINE BIOLOGY *RECEPTOR SITES(PHYSIOLOGY) *EVOLUTION(BIOLOGY) *REPRODUCTION(PHYSIOLOGY) MAMMALS ORGANIC MATERIALS GENES POLLUTANTS RESPONSE(BIOLOGY) WHALES LABORATORY ANIMALS AQUATIC ANIMALS SENSE ORGANS CULTURES(BIOLOGY) NORTH ATLANTIC OCEAN DIET CELLS(BIOLOGY) GENETIC ENGINEERING STEROIDS EXPOSURE(PHYSIOLOGY) CLONES ARYL RADICALS CONTAMINANTS CHEMICALS THESES |
description |
Marine mammals possess high body burdens of persistent organic pollutants, including PCBs and dioxin-like compounds (DLC). Chronic environmental or dietary exposure to these chemicals can disrupt the function of reproductive and immune systems in laboratory animals. The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor. mediating the expression of a suite of genes in response to exposure to DLC and structurally related chemicals. Species-specific differences in AHR structure can affect an organism's susceptibility to the effects of DLC. The structures and functions of several cetacean AHRs were investigated using In vitro molecular cloning and biochemical techniques. Using remote biopsy and molecular cloning methods, RNA was extracted from small integument samples from living North Atlantic right whales to identify the AHR cDNA sequence and other genes. Molecular and biochemical characteristics for North Atlantic right whale and humpback whale AHR cDNAs were determined using in vitro and cell culture methods. The properties of these AHRs were compared with those from other model and marine mammalian species using biochemical, phylogenetic. and homology modeling analyses. Additional studies are necessary to link exposure to environmental contaminants with potential reproductive effects in marine mammals, especially via interactions with steroid hormone receptor pathways. |
author2 |
MASSACHUSETTS INST OF TECH CAMBRIDGE JOINT PROGRAM IN APPLIED OCEAN SCIENCE AND ENGINEERING |
format |
Text |
author |
Lapseritis, Joy M. |
author_facet |
Lapseritis, Joy M. |
author_sort |
Lapseritis, Joy M. |
title |
Comparative Analyses of Aryl Hydrocarbon Receptor Structure, Function, and Evolution in Marine Mammals |
title_short |
Comparative Analyses of Aryl Hydrocarbon Receptor Structure, Function, and Evolution in Marine Mammals |
title_full |
Comparative Analyses of Aryl Hydrocarbon Receptor Structure, Function, and Evolution in Marine Mammals |
title_fullStr |
Comparative Analyses of Aryl Hydrocarbon Receptor Structure, Function, and Evolution in Marine Mammals |
title_full_unstemmed |
Comparative Analyses of Aryl Hydrocarbon Receptor Structure, Function, and Evolution in Marine Mammals |
title_sort |
comparative analyses of aryl hydrocarbon receptor structure, function, and evolution in marine mammals |
publishDate |
2007 |
url |
http://www.dtic.mil/docs/citations/ADA472638 http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=ADA472638 |
genre |
Humpback Whale North Atlantic North Atlantic right whale |
genre_facet |
Humpback Whale North Atlantic North Atlantic right whale |
op_source |
DTIC |
op_relation |
http://www.dtic.mil/docs/citations/ADA472638 |
op_rights |
Approved for public release; distribution is unlimited. |
_version_ |
1766026426223427584 |