Data from: Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake
RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for those infecti...
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ftdryad:oai:v1.datadryad.org:10255/dryad.90933 2023-05-15T14:00:52+02:00 Data from: Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake López-Bueno, Alberto Rastrojo, Alberto Peiró, Ramon Arenas, Miguel Alcamí, Antonio 2015-07-14T17:48:38Z http://hdl.handle.net/10255/dryad.90933 https://doi.org/10.5061/dryad.1dp6s unknown doi:10.5061/dryad.1dp6s/1 doi:10.5061/dryad.1dp6s/2 doi:10.5061/dryad.1dp6s/3 doi:10.5061/dryad.1dp6s/4 doi:10.5061/dryad.1dp6s/5 doi:10.1111/mec.13321 PMID:26198078 doi:10.5061/dryad.1dp6s López-Bueno A, Rastrojo A, Peiró R, Arenas M, Alcamí A (2015) Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake. Molecular Ecology 24(19): 4812–4825. http://hdl.handle.net/10255/dryad.90933 Metagenomics Microbial Biology Molecular Evolution Genomics/Proteomics Article 2015 ftdryad https://doi.org/10.5061/dryad.1dp6s https://doi.org/10.5061/dryad.1dp6s/1 https://doi.org/10.5061/dryad.1dp6s/2 https://doi.org/10.5061/dryad.1dp6s/3 https://doi.org/10.5061/dryad.1dp6s/4 https://doi.org/10.5061/dryad.1dp6s/5 https://doi.org/1 2020-01-01T15:21:33Z RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for those infecting micro-organisms in environmental samples. We report the first metagenomic study of RNA viruses in an Antarctic lake (Lake Limnopolar, Livingston Island). Similar to low-latitude aquatic environments, this lake harbours an RNA virome dominated by positive single-strand RNA viruses from the order Picornavirales probably infecting micro-organisms. Antarctic picorna-like virus 1 (APLV1), one of the most abundant viruses in the lake, does not incorporate any mutation in the consensus sequence from 2006 to 2010 and shows stable quasispecies with low-complexity indexes. By contrast, APLV2-APLV3 are detected in the lake water exclusively in summer samples and are major constituents of surrounding cyanobacterial mats. Their quasispecies exhibit low complexity in cyanobacterial mat, but their run-off-mediated transfer to the lake results in a remarkable increase of complexity that may reflect the convergence of different viral quasispecies from the catchment area or replication in a more diverse host community. This is the first example of viral quasispecies from natural aquatic ecosystems and points to ecological connectivity as a modulating factor of quasispecies complexity. Article in Journal/Newspaper Antarc* Antarctic Livingston Island Dryad Digital Repository (Duke University) Antarctic Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
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Open Polar |
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Dryad Digital Repository (Duke University) |
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topic |
Metagenomics Microbial Biology Molecular Evolution Genomics/Proteomics |
spellingShingle |
Metagenomics Microbial Biology Molecular Evolution Genomics/Proteomics López-Bueno, Alberto Rastrojo, Alberto Peiró, Ramon Arenas, Miguel Alcamí, Antonio Data from: Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
topic_facet |
Metagenomics Microbial Biology Molecular Evolution Genomics/Proteomics |
description |
RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for those infecting micro-organisms in environmental samples. We report the first metagenomic study of RNA viruses in an Antarctic lake (Lake Limnopolar, Livingston Island). Similar to low-latitude aquatic environments, this lake harbours an RNA virome dominated by positive single-strand RNA viruses from the order Picornavirales probably infecting micro-organisms. Antarctic picorna-like virus 1 (APLV1), one of the most abundant viruses in the lake, does not incorporate any mutation in the consensus sequence from 2006 to 2010 and shows stable quasispecies with low-complexity indexes. By contrast, APLV2-APLV3 are detected in the lake water exclusively in summer samples and are major constituents of surrounding cyanobacterial mats. Their quasispecies exhibit low complexity in cyanobacterial mat, but their run-off-mediated transfer to the lake results in a remarkable increase of complexity that may reflect the convergence of different viral quasispecies from the catchment area or replication in a more diverse host community. This is the first example of viral quasispecies from natural aquatic ecosystems and points to ecological connectivity as a modulating factor of quasispecies complexity. |
format |
Article in Journal/Newspaper |
author |
López-Bueno, Alberto Rastrojo, Alberto Peiró, Ramon Arenas, Miguel Alcamí, Antonio |
author_facet |
López-Bueno, Alberto Rastrojo, Alberto Peiró, Ramon Arenas, Miguel Alcamí, Antonio |
author_sort |
López-Bueno, Alberto |
title |
Data from: Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_short |
Data from: Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_full |
Data from: Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_fullStr |
Data from: Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_full_unstemmed |
Data from: Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_sort |
data from: ecological connectivity shapes quasispecies structure of rna viruses in an antarctic lake |
publishDate |
2015 |
url |
http://hdl.handle.net/10255/dryad.90933 https://doi.org/10.5061/dryad.1dp6s |
long_lat |
ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
geographic |
Antarctic Livingston Island |
geographic_facet |
Antarctic Livingston Island |
genre |
Antarc* Antarctic Livingston Island |
genre_facet |
Antarc* Antarctic Livingston Island |
op_relation |
doi:10.5061/dryad.1dp6s/1 doi:10.5061/dryad.1dp6s/2 doi:10.5061/dryad.1dp6s/3 doi:10.5061/dryad.1dp6s/4 doi:10.5061/dryad.1dp6s/5 doi:10.1111/mec.13321 PMID:26198078 doi:10.5061/dryad.1dp6s López-Bueno A, Rastrojo A, Peiró R, Arenas M, Alcamí A (2015) Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake. Molecular Ecology 24(19): 4812–4825. http://hdl.handle.net/10255/dryad.90933 |
op_doi |
https://doi.org/10.5061/dryad.1dp6s https://doi.org/10.5061/dryad.1dp6s/1 https://doi.org/10.5061/dryad.1dp6s/2 https://doi.org/10.5061/dryad.1dp6s/3 https://doi.org/10.5061/dryad.1dp6s/4 https://doi.org/10.5061/dryad.1dp6s/5 https://doi.org/1 |
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1766270257182277632 |