Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours
We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest full...
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ftdryad:oai:v1.datadryad.org:10255/dryad.66852 2023-05-15T17:44:33+02:00 Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours Tanentzap, Andrew J. Vicari, Mark Bazely, Dawn R. Sweden Ontario 2014-07-02T15:09:34Z http://hdl.handle.net/10255/dryad.66852 https://doi.org/10.5061/dryad.06hn6/3 unknown doi:10.5061/dryad.06hn6 doi:10.5061/dryad.06hn6/3 http://hdl.handle.net/10255/dryad.66852 http://creativecommons.org/publicdomain/zero/1.0/ CC0 PDM symbioses evolutionary ecology plant defences plant endophytes salivary compounds Dataset untilArticleAppears 2014 ftdryad https://doi.org/10.5061/dryad.06hn6/3 https://doi.org/10.5061/dryad.06hn6 2020-01-01T15:09:48Z We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest fully-extended leaf in each plant. Sections were surface sterilized and embedded in potato dextrose medium with 60 mg/L of penicillin G and streptomycin. Plates were stored in the dark at 24°C for 2 weeks. We then assigned 22-25 colonies per treatment, with 5-9 replicates of three plant genotypes, and recorded the initial radius of each plate. Saliva was applied and we measured colony radius relative to the baseline every 8 hours over 2 days. Report Northern Sweden Dryad Digital Repository (Duke University) |
institution |
Open Polar |
collection |
Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
symbioses evolutionary ecology plant defences plant endophytes salivary compounds |
spellingShingle |
symbioses evolutionary ecology plant defences plant endophytes salivary compounds Tanentzap, Andrew J. Vicari, Mark Bazely, Dawn R. Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours |
topic_facet |
symbioses evolutionary ecology plant defences plant endophytes salivary compounds |
description |
We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest fully-extended leaf in each plant. Sections were surface sterilized and embedded in potato dextrose medium with 60 mg/L of penicillin G and streptomycin. Plates were stored in the dark at 24°C for 2 weeks. We then assigned 22-25 colonies per treatment, with 5-9 replicates of three plant genotypes, and recorded the initial radius of each plate. Saliva was applied and we measured colony radius relative to the baseline every 8 hours over 2 days. |
format |
Report |
author |
Tanentzap, Andrew J. Vicari, Mark Bazely, Dawn R. |
author_facet |
Tanentzap, Andrew J. Vicari, Mark Bazely, Dawn R. |
author_sort |
Tanentzap, Andrew J. |
title |
Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours |
title_short |
Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours |
title_full |
Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours |
title_fullStr |
Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours |
title_full_unstemmed |
Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours |
title_sort |
radii of epichloë festucae colonies treated with saliva or water measured every 8 hours |
publishDate |
2014 |
url |
http://hdl.handle.net/10255/dryad.66852 https://doi.org/10.5061/dryad.06hn6/3 |
op_coverage |
Sweden Ontario |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
doi:10.5061/dryad.06hn6 doi:10.5061/dryad.06hn6/3 http://hdl.handle.net/10255/dryad.66852 |
op_rights |
http://creativecommons.org/publicdomain/zero/1.0/ |
op_rightsnorm |
CC0 PDM |
op_doi |
https://doi.org/10.5061/dryad.06hn6/3 https://doi.org/10.5061/dryad.06hn6 |
_version_ |
1766146790260736000 |