Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours
We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest full...
Main Authors: | , , |
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Format: | Report |
Language: | unknown |
Published: |
2014
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Subjects: | |
Online Access: | http://hdl.handle.net/10255/dryad.66852 https://doi.org/10.5061/dryad.06hn6/3 |
Summary: | We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest fully-extended leaf in each plant. Sections were surface sterilized and embedded in potato dextrose medium with 60 mg/L of penicillin G and streptomycin. Plates were stored in the dark at 24°C for 2 weeks. We then assigned 22-25 colonies per treatment, with 5-9 replicates of three plant genotypes, and recorded the initial radius of each plate. Saliva was applied and we measured colony radius relative to the baseline every 8 hours over 2 days. |
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