Data from: Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales
North Atlantic right whales have one of the lowest levels of genetic variation at minisatellite loci, microsatellite loci, and mitochondrial control region haplotypes among mammals. Here, adaptive variation at the peptide binding region of class I and class II DRB-like genes of the major histocompat...
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ftdryad:oai:v1.datadryad.org:10255/dryad.57058 2023-05-15T17:29:01+02:00 Data from: Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales Gillett, Roxanne M. Murray, Brent W. White, Bradley N. 2013-11-27T15:46:03Z http://hdl.handle.net/10255/dryad.57058 https://doi.org/10.5061/dryad.4d789 unknown doi:10.5061/dryad.4d789/1 doi:10.5061/dryad.4d789/2 doi:10.5061/dryad.4d789/3 doi:10.5061/dryad.4d789/4 doi:10.1093/jhered/est095 PMID:24381183 doi:10.5061/dryad.4d789 Gillett RM, Murray BW, White BN (2013) Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales. Journal of Heredity 105(2): 188-202. http://hdl.handle.net/10255/dryad.57058 MHC DRB haplotype North Atlantic right whale pedigree analysis Quantitative genetics and Mendelian inheritance conservation genetics biodiversity Article 2013 ftdryad https://doi.org/10.5061/dryad.4d789 https://doi.org/10.5061/dryad.4d789/1 https://doi.org/10.5061/dryad.4d789/2 https://doi.org/10.5061/dryad.4d789/3 https://doi.org/10.5061/dryad.4d789/4 https://doi.org/10.1093/jhered/est095 2020-01-01T15:05:10Z North Atlantic right whales have one of the lowest levels of genetic variation at minisatellite loci, microsatellite loci, and mitochondrial control region haplotypes among mammals. Here, adaptive variation at the peptide binding region of class I and class II DRB-like genes of the major histocompatibility complex was assessed. Amplification of a duplicated region in 222 individuals revealed at least 11 class II alleles. Six alleles were assigned to the locus Eugl-DRB1 and 5 alleles were assigned to the locus Eugl-DRB2 by assessing segregation patterns of alleles from 81 parent/offspring pedigrees. Pedigree analysis indicated that these alleles segregated into 12 distinct haplotypes. Genotyping a smaller subset of unrelated individuals (n = 5 and 10, respectively) using different primer sets revealed at least 2 class II pseudogenes (with ≥ 4 alleles) and at least 3 class I loci (with ≥ 6 alleles). Class II sequences were significantly different from neutrality at peptide binding sites suggesting loci may be under the influence of balancing selection. Trans-species sharing of alleles was apparent for class I and class II sequences. Characterization of class II loci represents the first step in determining the relationship between major histocompatibility complex variability and factors affecting health and reproduction in this species. Article in Journal/Newspaper North Atlantic North Atlantic right whale Dryad Digital Repository (Duke University) |
institution |
Open Polar |
collection |
Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
MHC DRB haplotype North Atlantic right whale pedigree analysis Quantitative genetics and Mendelian inheritance conservation genetics biodiversity |
spellingShingle |
MHC DRB haplotype North Atlantic right whale pedigree analysis Quantitative genetics and Mendelian inheritance conservation genetics biodiversity Gillett, Roxanne M. Murray, Brent W. White, Bradley N. Data from: Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales |
topic_facet |
MHC DRB haplotype North Atlantic right whale pedigree analysis Quantitative genetics and Mendelian inheritance conservation genetics biodiversity |
description |
North Atlantic right whales have one of the lowest levels of genetic variation at minisatellite loci, microsatellite loci, and mitochondrial control region haplotypes among mammals. Here, adaptive variation at the peptide binding region of class I and class II DRB-like genes of the major histocompatibility complex was assessed. Amplification of a duplicated region in 222 individuals revealed at least 11 class II alleles. Six alleles were assigned to the locus Eugl-DRB1 and 5 alleles were assigned to the locus Eugl-DRB2 by assessing segregation patterns of alleles from 81 parent/offspring pedigrees. Pedigree analysis indicated that these alleles segregated into 12 distinct haplotypes. Genotyping a smaller subset of unrelated individuals (n = 5 and 10, respectively) using different primer sets revealed at least 2 class II pseudogenes (with ≥ 4 alleles) and at least 3 class I loci (with ≥ 6 alleles). Class II sequences were significantly different from neutrality at peptide binding sites suggesting loci may be under the influence of balancing selection. Trans-species sharing of alleles was apparent for class I and class II sequences. Characterization of class II loci represents the first step in determining the relationship between major histocompatibility complex variability and factors affecting health and reproduction in this species. |
format |
Article in Journal/Newspaper |
author |
Gillett, Roxanne M. Murray, Brent W. White, Bradley N. |
author_facet |
Gillett, Roxanne M. Murray, Brent W. White, Bradley N. |
author_sort |
Gillett, Roxanne M. |
title |
Data from: Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales |
title_short |
Data from: Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales |
title_full |
Data from: Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales |
title_fullStr |
Data from: Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales |
title_full_unstemmed |
Data from: Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales |
title_sort |
data from: characterization of class i- and ii-like major histocompatibility complex loci in pedigrees of north atlantic right whales |
publishDate |
2013 |
url |
http://hdl.handle.net/10255/dryad.57058 https://doi.org/10.5061/dryad.4d789 |
genre |
North Atlantic North Atlantic right whale |
genre_facet |
North Atlantic North Atlantic right whale |
op_relation |
doi:10.5061/dryad.4d789/1 doi:10.5061/dryad.4d789/2 doi:10.5061/dryad.4d789/3 doi:10.5061/dryad.4d789/4 doi:10.1093/jhered/est095 PMID:24381183 doi:10.5061/dryad.4d789 Gillett RM, Murray BW, White BN (2013) Characterization of class I- and II-like major histocompatibility complex loci in pedigrees of North Atlantic right whales. Journal of Heredity 105(2): 188-202. http://hdl.handle.net/10255/dryad.57058 |
op_doi |
https://doi.org/10.5061/dryad.4d789 https://doi.org/10.5061/dryad.4d789/1 https://doi.org/10.5061/dryad.4d789/2 https://doi.org/10.5061/dryad.4d789/3 https://doi.org/10.5061/dryad.4d789/4 https://doi.org/10.1093/jhered/est095 |
_version_ |
1766122325187493888 |