Data from: The use of archived tags in retrospective genetic analysis of fish

Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populatio...

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Main Authors: Bonanomi, Sara, Overgaard Therkildsen, Nina, Berg Hedeholm, Rasmus, Hemmer-Hansen, Jakob, Nielsen, Einar Eg
Format: Article in Journal/Newspaper
Language:unknown
Published: 2013
Subjects:
Online Access:http://hdl.handle.net/10255/dryad.56877
https://doi.org/10.5061/dryad.sp0ks
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record_format openpolar
spelling ftdryad:oai:v1.datadryad.org:10255/dryad.56877 2023-05-15T15:27:28+02:00 Data from: The use of archived tags in retrospective genetic analysis of fish Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg 2013-12-05T16:32:56Z http://hdl.handle.net/10255/dryad.56877 https://doi.org/10.5061/dryad.sp0ks unknown doi:10.5061/dryad.sp0ks/1 doi:10.1111/1755-0998.12211 PMID:24299474 doi:10.5061/dryad.sp0ks Bonanomi S, Overgaard Therkildsen N, Berg Hedeholm R, Hemmer-Hansen J, Nielsen EE (2013) The use of archived tags in retrospective genetic analysis of fish. Molecular Ecology Resources 14(3): 616–621. http://hdl.handle.net/10255/dryad.56877 historical samples fish tags Atlantic cod temporal genetic analysis DNA extraction Article 2013 ftdryad https://doi.org/10.5061/dryad.sp0ks https://doi.org/10.5061/dryad.sp0ks/1 https://doi.org/10.1111/1755-0998.12211 2020-01-01T15:05:07Z Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish. Article in Journal/Newspaper atlantic cod greenlandic Dryad Digital Repository (Duke University)
institution Open Polar
collection Dryad Digital Repository (Duke University)
op_collection_id ftdryad
language unknown
topic historical samples
fish tags
Atlantic cod
temporal genetic analysis
DNA extraction
spellingShingle historical samples
fish tags
Atlantic cod
temporal genetic analysis
DNA extraction
Bonanomi, Sara
Overgaard Therkildsen, Nina
Berg Hedeholm, Rasmus
Hemmer-Hansen, Jakob
Nielsen, Einar Eg
Data from: The use of archived tags in retrospective genetic analysis of fish
topic_facet historical samples
fish tags
Atlantic cod
temporal genetic analysis
DNA extraction
description Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish.
format Article in Journal/Newspaper
author Bonanomi, Sara
Overgaard Therkildsen, Nina
Berg Hedeholm, Rasmus
Hemmer-Hansen, Jakob
Nielsen, Einar Eg
author_facet Bonanomi, Sara
Overgaard Therkildsen, Nina
Berg Hedeholm, Rasmus
Hemmer-Hansen, Jakob
Nielsen, Einar Eg
author_sort Bonanomi, Sara
title Data from: The use of archived tags in retrospective genetic analysis of fish
title_short Data from: The use of archived tags in retrospective genetic analysis of fish
title_full Data from: The use of archived tags in retrospective genetic analysis of fish
title_fullStr Data from: The use of archived tags in retrospective genetic analysis of fish
title_full_unstemmed Data from: The use of archived tags in retrospective genetic analysis of fish
title_sort data from: the use of archived tags in retrospective genetic analysis of fish
publishDate 2013
url http://hdl.handle.net/10255/dryad.56877
https://doi.org/10.5061/dryad.sp0ks
genre atlantic cod
greenlandic
genre_facet atlantic cod
greenlandic
op_relation doi:10.5061/dryad.sp0ks/1
doi:10.1111/1755-0998.12211
PMID:24299474
doi:10.5061/dryad.sp0ks
Bonanomi S, Overgaard Therkildsen N, Berg Hedeholm R, Hemmer-Hansen J, Nielsen EE (2013) The use of archived tags in retrospective genetic analysis of fish. Molecular Ecology Resources 14(3): 616–621.
http://hdl.handle.net/10255/dryad.56877
op_doi https://doi.org/10.5061/dryad.sp0ks
https://doi.org/10.5061/dryad.sp0ks/1
https://doi.org/10.1111/1755-0998.12211
_version_ 1766357896942059520