Data from: The use of archived tags in retrospective genetic analysis of fish
Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populatio...
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Online Access: | http://hdl.handle.net/10255/dryad.56877 https://doi.org/10.5061/dryad.sp0ks |
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ftdryad:oai:v1.datadryad.org:10255/dryad.56877 2023-05-15T15:27:28+02:00 Data from: The use of archived tags in retrospective genetic analysis of fish Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg 2013-12-05T16:32:56Z http://hdl.handle.net/10255/dryad.56877 https://doi.org/10.5061/dryad.sp0ks unknown doi:10.5061/dryad.sp0ks/1 doi:10.1111/1755-0998.12211 PMID:24299474 doi:10.5061/dryad.sp0ks Bonanomi S, Overgaard Therkildsen N, Berg Hedeholm R, Hemmer-Hansen J, Nielsen EE (2013) The use of archived tags in retrospective genetic analysis of fish. Molecular Ecology Resources 14(3): 616–621. http://hdl.handle.net/10255/dryad.56877 historical samples fish tags Atlantic cod temporal genetic analysis DNA extraction Article 2013 ftdryad https://doi.org/10.5061/dryad.sp0ks https://doi.org/10.5061/dryad.sp0ks/1 https://doi.org/10.1111/1755-0998.12211 2020-01-01T15:05:07Z Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish. Article in Journal/Newspaper atlantic cod greenlandic Dryad Digital Repository (Duke University) |
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Open Polar |
collection |
Dryad Digital Repository (Duke University) |
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topic |
historical samples fish tags Atlantic cod temporal genetic analysis DNA extraction |
spellingShingle |
historical samples fish tags Atlantic cod temporal genetic analysis DNA extraction Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg Data from: The use of archived tags in retrospective genetic analysis of fish |
topic_facet |
historical samples fish tags Atlantic cod temporal genetic analysis DNA extraction |
description |
Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish. |
format |
Article in Journal/Newspaper |
author |
Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg |
author_facet |
Bonanomi, Sara Overgaard Therkildsen, Nina Berg Hedeholm, Rasmus Hemmer-Hansen, Jakob Nielsen, Einar Eg |
author_sort |
Bonanomi, Sara |
title |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_short |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_full |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_fullStr |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_full_unstemmed |
Data from: The use of archived tags in retrospective genetic analysis of fish |
title_sort |
data from: the use of archived tags in retrospective genetic analysis of fish |
publishDate |
2013 |
url |
http://hdl.handle.net/10255/dryad.56877 https://doi.org/10.5061/dryad.sp0ks |
genre |
atlantic cod greenlandic |
genre_facet |
atlantic cod greenlandic |
op_relation |
doi:10.5061/dryad.sp0ks/1 doi:10.1111/1755-0998.12211 PMID:24299474 doi:10.5061/dryad.sp0ks Bonanomi S, Overgaard Therkildsen N, Berg Hedeholm R, Hemmer-Hansen J, Nielsen EE (2013) The use of archived tags in retrospective genetic analysis of fish. Molecular Ecology Resources 14(3): 616–621. http://hdl.handle.net/10255/dryad.56877 |
op_doi |
https://doi.org/10.5061/dryad.sp0ks https://doi.org/10.5061/dryad.sp0ks/1 https://doi.org/10.1111/1755-0998.12211 |
_version_ |
1766357896942059520 |