Data from: Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn
Northern freshwater fish may be suitable for the genetic dissection of ecological traits because they invaded new habitats after the last ice age (~10.000 years ago). Arctic charr (Salvelinus alpinus) colonizing streams and lakes in Iceland gave rise to multiple populations of small benthic morphoty...
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Online Access: | http://hdl.handle.net/10255/dryad.50946 https://doi.org/10.5061/dryad.81884 |
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ftdryad:oai:v1.datadryad.org:10255/dryad.50946 2023-05-15T14:30:03+02:00 Data from: Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn Kapralova, Kalina H. Gudbrandsson, Johannes Reynisdottir, Sigrun Santos, Cristina B. Baltanás, Vanessa C. Maier, Valerie H. Snorrason, Sigurdur S. Palsson, Arnar Iceland 2013-07-25T17:45:42Z http://hdl.handle.net/10255/dryad.50946 https://doi.org/10.5061/dryad.81884 unknown doi:10.5061/dryad.81884/1 doi:10.5061/dryad.81884/2 doi:10.1371/journal.pone.0069402 PMID:23894470 doi:10.5061/dryad.81884 Kapralova KH, Gudbrandsson J, Reynisdottir S, Santos CB, Baltanás VC, Maier VH, Snorrason SS, Palsson A (2013) Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn. PLoS ONE 8(7): e69402. http://hdl.handle.net/10255/dryad.50946 association test Immunology evolutionary genetics parasites Article 2013 ftdryad https://doi.org/10.5061/dryad.81884 https://doi.org/10.5061/dryad.81884/1 https://doi.org/10.5061/dryad.81884/2 https://doi.org/10.1371/journal.pone.0069402 2020-01-01T15:02:14Z Northern freshwater fish may be suitable for the genetic dissection of ecological traits because they invaded new habitats after the last ice age (~10.000 years ago). Arctic charr (Salvelinus alpinus) colonizing streams and lakes in Iceland gave rise to multiple populations of small benthic morphotypes, often in sympatry with a pelagic morphotype. Earlier studies have revealed significant, but subtle, genetic differentiation between the three most common morphs in Lake Thingvallavatn. We conducted a population genetic screen on four immunological candidate genes Cathelicidin 2 (Cath2), Hepcidin (Hamp), Liver expressed antimicrobial peptide 2a (Leap-2a), and Major Histocompatibility Complex IIα (MHCIIα) and a mitochondrial marker (D-loop) among the three most common Lake Thingvallavatn charr morphs. Significant differences in allele frequencies were found between morphs at the Cath2 and MHCIIα loci. No such signal was detected in the D-loop nor in the other two immunological genes. In Cath2 the small benthic morph deviated from the other two (FST = 0.13), one of the substitutions detected constituting an amino acid replacement polymorphism in the antimicrobial peptide. A more striking difference was found in the MHCIIα. Two haplotypes were very common in the lake, and their frequency differed greatly between the morphotypes (from 22% to 93.5%, FST = 0.67). We then expanded our study by surveying the variation in Cath2 and MHCIIα in 9 Arctic charr populations from around Iceland. The populations varied greatly in terms of allele frequencies at Cath2, but the variation did not correlate with morphotype. At the MHCIIα locus, the variation was nearly identical to the variation in the two benthic morphs of Lake Thingvallavatn. The results are consistent with a scenario where parts of the immune systems have diverged substantially among Arctic charr populations in Iceland, after colonizing the island ~10.000 years ago. Article in Journal/Newspaper Arctic charr Arctic Iceland Salvelinus alpinus Dryad Digital Repository (Duke University) Arctic |
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Open Polar |
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Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
association test Immunology evolutionary genetics parasites |
spellingShingle |
association test Immunology evolutionary genetics parasites Kapralova, Kalina H. Gudbrandsson, Johannes Reynisdottir, Sigrun Santos, Cristina B. Baltanás, Vanessa C. Maier, Valerie H. Snorrason, Sigurdur S. Palsson, Arnar Data from: Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn |
topic_facet |
association test Immunology evolutionary genetics parasites |
description |
Northern freshwater fish may be suitable for the genetic dissection of ecological traits because they invaded new habitats after the last ice age (~10.000 years ago). Arctic charr (Salvelinus alpinus) colonizing streams and lakes in Iceland gave rise to multiple populations of small benthic morphotypes, often in sympatry with a pelagic morphotype. Earlier studies have revealed significant, but subtle, genetic differentiation between the three most common morphs in Lake Thingvallavatn. We conducted a population genetic screen on four immunological candidate genes Cathelicidin 2 (Cath2), Hepcidin (Hamp), Liver expressed antimicrobial peptide 2a (Leap-2a), and Major Histocompatibility Complex IIα (MHCIIα) and a mitochondrial marker (D-loop) among the three most common Lake Thingvallavatn charr morphs. Significant differences in allele frequencies were found between morphs at the Cath2 and MHCIIα loci. No such signal was detected in the D-loop nor in the other two immunological genes. In Cath2 the small benthic morph deviated from the other two (FST = 0.13), one of the substitutions detected constituting an amino acid replacement polymorphism in the antimicrobial peptide. A more striking difference was found in the MHCIIα. Two haplotypes were very common in the lake, and their frequency differed greatly between the morphotypes (from 22% to 93.5%, FST = 0.67). We then expanded our study by surveying the variation in Cath2 and MHCIIα in 9 Arctic charr populations from around Iceland. The populations varied greatly in terms of allele frequencies at Cath2, but the variation did not correlate with morphotype. At the MHCIIα locus, the variation was nearly identical to the variation in the two benthic morphs of Lake Thingvallavatn. The results are consistent with a scenario where parts of the immune systems have diverged substantially among Arctic charr populations in Iceland, after colonizing the island ~10.000 years ago. |
format |
Article in Journal/Newspaper |
author |
Kapralova, Kalina H. Gudbrandsson, Johannes Reynisdottir, Sigrun Santos, Cristina B. Baltanás, Vanessa C. Maier, Valerie H. Snorrason, Sigurdur S. Palsson, Arnar |
author_facet |
Kapralova, Kalina H. Gudbrandsson, Johannes Reynisdottir, Sigrun Santos, Cristina B. Baltanás, Vanessa C. Maier, Valerie H. Snorrason, Sigurdur S. Palsson, Arnar |
author_sort |
Kapralova, Kalina H. |
title |
Data from: Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn |
title_short |
Data from: Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn |
title_full |
Data from: Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn |
title_fullStr |
Data from: Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn |
title_full_unstemmed |
Data from: Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn |
title_sort |
data from: differentiation at the mhciiα and cath2 loci in sympatric salvelinus alpinus resource morphs in lake thingvallavatn |
publishDate |
2013 |
url |
http://hdl.handle.net/10255/dryad.50946 https://doi.org/10.5061/dryad.81884 |
op_coverage |
Iceland |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Iceland Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Iceland Salvelinus alpinus |
op_relation |
doi:10.5061/dryad.81884/1 doi:10.5061/dryad.81884/2 doi:10.1371/journal.pone.0069402 PMID:23894470 doi:10.5061/dryad.81884 Kapralova KH, Gudbrandsson J, Reynisdottir S, Santos CB, Baltanás VC, Maier VH, Snorrason SS, Palsson A (2013) Differentiation at the MHCIIα and Cath2 loci in sympatric Salvelinus alpinus resource morphs in Lake Thingvallavatn. PLoS ONE 8(7): e69402. http://hdl.handle.net/10255/dryad.50946 |
op_doi |
https://doi.org/10.5061/dryad.81884 https://doi.org/10.5061/dryad.81884/1 https://doi.org/10.5061/dryad.81884/2 https://doi.org/10.1371/journal.pone.0069402 |
_version_ |
1766303983917334528 |