Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus
Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption (ED) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapi...
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ftdryad:oai:v1.datadryad.org:10255/dryad.43295 2023-05-15T18:49:53+02:00 Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon 2013-07-03T19:42:20Z http://hdl.handle.net/10255/dryad.43295 https://doi.org/10.5061/dryad.m1063 unknown doi:10.5061/dryad.m1063/1 doi:10.5061/dryad.m1063/2 doi:10.1111/mec.12137 PMID:23205577 doi:10.5061/dryad.m1063 Pascoal S, Carvalho G, Vasieva O, Hughes R, Cossins A, Fang Y, Ashelford K, Olohan L, Barroso C, Mendo S, Creer S (2012) Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus. Molecular Ecology 22(6): 1589–1608. http://hdl.handle.net/10255/dryad.43295 Ecological Genetics Gene Structure and Function Genomics/Proteomics Invertebrates Molecular Evolution Transcriptomics Article 2013 ftdryad https://doi.org/10.5061/dryad.m1063 https://doi.org/10.5061/dryad.m1063/1 https://doi.org/10.5061/dryad.m1063/2 https://doi.org/10.1111/mec.12137 2020-01-01T14:58:21Z Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption (ED) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapillus (imposex), caused by organotins, provides one of the most clearcut ecological examples of anthropogenically induced ED in aquatic ecosystems. To identify the underpinning mechanisms of imposex for this ‘nonmodel’ species, we combined Roche 454 pyrosequencing with custom oligoarray fabrication inexpensively to both generate gene models and identify those responding to chronic tributyltin (TBT) treatment. The results supported the involvement of steroid, neuroendocrine peptide hormone dysfunction and retinoid mechanisms, but suggested additionally the involvement of putative peroxisome proliferator–activated receptor (PPAR) pathways. Application of rosiglitazone, a well-known vertebrate PPARγ ligand, to dogwhelks induced imposex in the absence of TBT. Thus, while TBT-induced imposex is linked to the induction of many genes and has a complex phenotype, it is likely also to be driven by PPAR-responsive pathways, hitherto not described in invertebrates. Our findings provide further evidence for a common signalling pathway between invertebrate and vertebrate species that has previously been overlooked in the study of endocrine disruption. Article in Journal/Newspaper Dogwhelk Nucella lapillus Dryad Digital Repository (Duke University) |
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Open Polar |
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Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
Ecological Genetics Gene Structure and Function Genomics/Proteomics Invertebrates Molecular Evolution Transcriptomics |
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Ecological Genetics Gene Structure and Function Genomics/Proteomics Invertebrates Molecular Evolution Transcriptomics Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
topic_facet |
Ecological Genetics Gene Structure and Function Genomics/Proteomics Invertebrates Molecular Evolution Transcriptomics |
description |
Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption (ED) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapillus (imposex), caused by organotins, provides one of the most clearcut ecological examples of anthropogenically induced ED in aquatic ecosystems. To identify the underpinning mechanisms of imposex for this ‘nonmodel’ species, we combined Roche 454 pyrosequencing with custom oligoarray fabrication inexpensively to both generate gene models and identify those responding to chronic tributyltin (TBT) treatment. The results supported the involvement of steroid, neuroendocrine peptide hormone dysfunction and retinoid mechanisms, but suggested additionally the involvement of putative peroxisome proliferator–activated receptor (PPAR) pathways. Application of rosiglitazone, a well-known vertebrate PPARγ ligand, to dogwhelks induced imposex in the absence of TBT. Thus, while TBT-induced imposex is linked to the induction of many genes and has a complex phenotype, it is likely also to be driven by PPAR-responsive pathways, hitherto not described in invertebrates. Our findings provide further evidence for a common signalling pathway between invertebrate and vertebrate species that has previously been overlooked in the study of endocrine disruption. |
format |
Article in Journal/Newspaper |
author |
Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon |
author_facet |
Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon |
author_sort |
Pascoal, Sonia |
title |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_short |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_full |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_fullStr |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_full_unstemmed |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_sort |
data from: transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, nucella lapillus |
publishDate |
2013 |
url |
http://hdl.handle.net/10255/dryad.43295 https://doi.org/10.5061/dryad.m1063 |
genre |
Dogwhelk Nucella lapillus |
genre_facet |
Dogwhelk Nucella lapillus |
op_relation |
doi:10.5061/dryad.m1063/1 doi:10.5061/dryad.m1063/2 doi:10.1111/mec.12137 PMID:23205577 doi:10.5061/dryad.m1063 Pascoal S, Carvalho G, Vasieva O, Hughes R, Cossins A, Fang Y, Ashelford K, Olohan L, Barroso C, Mendo S, Creer S (2012) Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus. Molecular Ecology 22(6): 1589–1608. http://hdl.handle.net/10255/dryad.43295 |
op_doi |
https://doi.org/10.5061/dryad.m1063 https://doi.org/10.5061/dryad.m1063/1 https://doi.org/10.5061/dryad.m1063/2 https://doi.org/10.1111/mec.12137 |
_version_ |
1766243502769831936 |