genotype_age_location_Dryad2012
American glass eels recruitment begins in Florida around December and progresses northward to Newfoundland–Labrador until June/July (Helfman et al. 1987). The first waves of glass eels at each location were sampled in 2008 following this latitudinal trend at 17 sites evenly distributed along eastern...
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Format: | Report |
Language: | unknown |
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2012
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Online Access: | http://hdl.handle.net/10255/dryad.42515 https://doi.org/10.5061/dryad.39jb0/1 |
id |
ftdryad:oai:v1.datadryad.org:10255/dryad.42515 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
Conservation Biology Conservation Genetics Ecological Genetics Fisheries Management Population Genetics - Empirical Wildlife Management |
spellingShingle |
Conservation Biology Conservation Genetics Ecological Genetics Fisheries Management Population Genetics - Empirical Wildlife Management Côté, Caroline Castonguay, Martin Gagnaire, Pierre-Alexandre Bourret, Vincent Verreault, Guy Bernatchez, Louis genotype_age_location_Dryad2012 |
topic_facet |
Conservation Biology Conservation Genetics Ecological Genetics Fisheries Management Population Genetics - Empirical Wildlife Management |
description |
American glass eels recruitment begins in Florida around December and progresses northward to Newfoundland–Labrador until June/July (Helfman et al. 1987). The first waves of glass eels at each location were sampled in 2008 following this latitudinal trend at 17 sites evenly distributed along eastern North America up to the St. Lawrence estuary. For each location, 50 individuals were measured and preserved in 95% ethanol. Yellow eels were also collected between May and September 2008 at 15 locations ranging from the upper St. Lawrence River to the Atlantic coast of Canada. The emphasis on yellow eel sampling in this region was motivated by the occurrence of strikingly different recruitment trends reported between Atlantic Canada versus the upper St. Lawrence River and Lake Ontario (Cairns et al. 2008). Sample size varied from 69 to 100 yellow eels per location. Fin clips were preserved in 95% ethanol for DNA extraction and genotyping, and heads were kept for otolith extraction. Yellow Eels collected in 2007 were finally rejected since they were frozen before being preserved in ethanol, wich caused a bias in microsattelits amplification. AGE DETERMINATION: Age was determined for 946 yellow eels, which allowed us to subdivide them into annual cohorts. Sagittal otoliths were extracted, stored in glass vials in a 95% ethanol: glycerine solution (1 : 1 ratio), and cleaned with successive baths of bleach, water, and 95% ethanol. Once dried, otoliths were embedded in a mix of epoxy resin and hardener (4:1 ratio) inside gelatine capsules for 24 h, ground to the core on the sagittal plane, and polished with alumina powder on a polishing disc. Sections were etched, decalcified in 5% EDTA for annuli enhancing, stained in 0.01% toluidine blue solution, and digitally photographed (e.g., Tremblay 2009). The first annulus after the dark central nucleus was considered as the elver check (metamorphosis from leptochephalus larva to glass eel) and subsequent annuli as winter checks (e.g., ICES 2011). Eels were considered by convention to be of age 0 + in their year of arrival in continental waters, and their “cohort year” was thus defined as so. Each otolith was aged twice by two eel experts to confirm aging. A total of 946 yellow eel otoliths were readable, representing 17 different cohorts, each comprising 1 to 127 individuals. |
format |
Report |
author |
Côté, Caroline Castonguay, Martin Gagnaire, Pierre-Alexandre Bourret, Vincent Verreault, Guy Bernatchez, Louis |
author_facet |
Côté, Caroline Castonguay, Martin Gagnaire, Pierre-Alexandre Bourret, Vincent Verreault, Guy Bernatchez, Louis |
author_sort |
Côté, Caroline |
title |
genotype_age_location_Dryad2012 |
title_short |
genotype_age_location_Dryad2012 |
title_full |
genotype_age_location_Dryad2012 |
title_fullStr |
genotype_age_location_Dryad2012 |
title_full_unstemmed |
genotype_age_location_Dryad2012 |
title_sort |
genotype_age_location_dryad2012 |
publishDate |
2012 |
url |
http://hdl.handle.net/10255/dryad.42515 https://doi.org/10.5061/dryad.39jb0/1 |
op_coverage |
Florida 30.02N -81.33W Guana River Dam Georgia 31.31N -81.47W Mornings-AR South Carolina 32.93N -80.01W Cooper River North Carolina 34.77N -76.81W Black Creek Virginia 37.22N -76.49W Wormley Creek York Delaware 38.59N -75.29W Millsboro Pond Spillway New Jersey 39.56N -74.58W Patcong Creak Linwood Pennsylvania 40.05N -74.98W Crum Creek Connecticut 41.30N -72.40W Tyler River Massachusetts 41.68N -70.92W Parker River New Hampshire 42.93N -70.86W Tayler River Nova Scotia 44.59N -64.17W Mira River Newfoundland 47.84N -59.26W Codroy River Québec 48.78N -67.69W Grande Rivière Blanche Prince Edward Island 46.43N -63.24W Rustico Bay Ontario 45.01N -74.79W Moses-Saunders Dam Québec 45.31N -73.90W Beauharnois Dam Québec 45.44N -73.26W Chambly Dam Québec 48.28N -68.95W Rivière du Sud-Ouest Québec 49.52N -67.28W Rivière de la Petite Trinité Québec 48.82N -64.83W Rivière Saint-Jean Maine 43.84N -69.65W West Harbor Pond New Brunswick 47.52N -64.91W Tracadie River New Brunswick 47.09N -65.22W Miramichi Estuary New Brunswick 45.87N -66.15W Grand-Lake Nova Scotia 44.36N -64.46W La Have River Nova Scotia 45.84N -60.80W Bras d’Or Lake Nova Scotia 46.43N -61.10W Margaree Harbour Newfoundland 47.60N -53.26W Roberts Bay Mesozoic |
long_lat |
ENVELOPE(140.018,140.018,-66.663,-66.663) ENVELOPE(162.150,162.150,-76.600,-76.600) ENVELOPE(-115.002,-115.002,58.384,58.384) ENVELOPE(10.500,10.500,-70.417,-70.417) ENVELOPE(-99.183,-99.183,-74.550,-74.550) ENVELOPE(70.132,70.132,-49.346,-49.346) ENVELOPE(-99.064,-99.064,57.290,57.290) ENVELOPE(-45.316,-45.316,-60.700,-60.700) ENVELOPE(-120.853,-120.853,55.783,55.783) |
geographic |
Blanche Canada Creak Lawrence River Mira Moses Newfoundland Rivière du Sud Roberts Bay Saunders Tremblay |
geographic_facet |
Blanche Canada Creak Lawrence River Mira Moses Newfoundland Rivière du Sud Roberts Bay Saunders Tremblay |
genre |
Newfoundland Prince Edward Island |
genre_facet |
Newfoundland Prince Edward Island |
op_relation |
doi:10.5061/dryad.39jb0 doi:10.5061/dryad.39jb0/1 http://hdl.handle.net/10255/dryad.42515 |
op_rights |
http://creativecommons.org/publicdomain/zero/1.0/ |
op_rightsnorm |
CC0 PDM |
op_doi |
https://doi.org/10.5061/dryad.39jb0/1 https://doi.org/10.5061/dryad.39jb0 |
_version_ |
1766108664694833152 |
spelling |
ftdryad:oai:v1.datadryad.org:10255/dryad.42515 2023-05-15T17:22:13+02:00 genotype_age_location_Dryad2012 Côté, Caroline Castonguay, Martin Gagnaire, Pierre-Alexandre Bourret, Vincent Verreault, Guy Bernatchez, Louis Florida 30.02N -81.33W Guana River Dam Georgia 31.31N -81.47W Mornings-AR South Carolina 32.93N -80.01W Cooper River North Carolina 34.77N -76.81W Black Creek Virginia 37.22N -76.49W Wormley Creek York Delaware 38.59N -75.29W Millsboro Pond Spillway New Jersey 39.56N -74.58W Patcong Creak Linwood Pennsylvania 40.05N -74.98W Crum Creek Connecticut 41.30N -72.40W Tyler River Massachusetts 41.68N -70.92W Parker River New Hampshire 42.93N -70.86W Tayler River Nova Scotia 44.59N -64.17W Mira River Newfoundland 47.84N -59.26W Codroy River Québec 48.78N -67.69W Grande Rivière Blanche Prince Edward Island 46.43N -63.24W Rustico Bay Ontario 45.01N -74.79W Moses-Saunders Dam Québec 45.31N -73.90W Beauharnois Dam Québec 45.44N -73.26W Chambly Dam Québec 48.28N -68.95W Rivière du Sud-Ouest Québec 49.52N -67.28W Rivière de la Petite Trinité Québec 48.82N -64.83W Rivière Saint-Jean Maine 43.84N -69.65W West Harbor Pond New Brunswick 47.52N -64.91W Tracadie River New Brunswick 47.09N -65.22W Miramichi Estuary New Brunswick 45.87N -66.15W Grand-Lake Nova Scotia 44.36N -64.46W La Have River Nova Scotia 45.84N -60.80W Bras d’Or Lake Nova Scotia 46.43N -61.10W Margaree Harbour Newfoundland 47.60N -53.26W Roberts Bay Mesozoic 2012-10-25T17:48:50Z http://hdl.handle.net/10255/dryad.42515 https://doi.org/10.5061/dryad.39jb0/1 unknown doi:10.5061/dryad.39jb0 doi:10.5061/dryad.39jb0/1 http://hdl.handle.net/10255/dryad.42515 http://creativecommons.org/publicdomain/zero/1.0/ CC0 PDM Conservation Biology Conservation Genetics Ecological Genetics Fisheries Management Population Genetics - Empirical Wildlife Management Dataset untilArticleAppears 2012 ftdryad https://doi.org/10.5061/dryad.39jb0/1 https://doi.org/10.5061/dryad.39jb0 2020-01-01T14:57:58Z American glass eels recruitment begins in Florida around December and progresses northward to Newfoundland–Labrador until June/July (Helfman et al. 1987). The first waves of glass eels at each location were sampled in 2008 following this latitudinal trend at 17 sites evenly distributed along eastern North America up to the St. Lawrence estuary. For each location, 50 individuals were measured and preserved in 95% ethanol. Yellow eels were also collected between May and September 2008 at 15 locations ranging from the upper St. Lawrence River to the Atlantic coast of Canada. The emphasis on yellow eel sampling in this region was motivated by the occurrence of strikingly different recruitment trends reported between Atlantic Canada versus the upper St. Lawrence River and Lake Ontario (Cairns et al. 2008). Sample size varied from 69 to 100 yellow eels per location. Fin clips were preserved in 95% ethanol for DNA extraction and genotyping, and heads were kept for otolith extraction. Yellow Eels collected in 2007 were finally rejected since they were frozen before being preserved in ethanol, wich caused a bias in microsattelits amplification. AGE DETERMINATION: Age was determined for 946 yellow eels, which allowed us to subdivide them into annual cohorts. Sagittal otoliths were extracted, stored in glass vials in a 95% ethanol: glycerine solution (1 : 1 ratio), and cleaned with successive baths of bleach, water, and 95% ethanol. Once dried, otoliths were embedded in a mix of epoxy resin and hardener (4:1 ratio) inside gelatine capsules for 24 h, ground to the core on the sagittal plane, and polished with alumina powder on a polishing disc. Sections were etched, decalcified in 5% EDTA for annuli enhancing, stained in 0.01% toluidine blue solution, and digitally photographed (e.g., Tremblay 2009). The first annulus after the dark central nucleus was considered as the elver check (metamorphosis from leptochephalus larva to glass eel) and subsequent annuli as winter checks (e.g., ICES 2011). Eels were considered by convention to be of age 0 + in their year of arrival in continental waters, and their “cohort year” was thus defined as so. Each otolith was aged twice by two eel experts to confirm aging. A total of 946 yellow eel otoliths were readable, representing 17 different cohorts, each comprising 1 to 127 individuals. Report Newfoundland Prince Edward Island Dryad Digital Repository (Duke University) Blanche ENVELOPE(140.018,140.018,-66.663,-66.663) Canada Creak ENVELOPE(162.150,162.150,-76.600,-76.600) Lawrence River ENVELOPE(-115.002,-115.002,58.384,58.384) Mira ENVELOPE(10.500,10.500,-70.417,-70.417) Moses ENVELOPE(-99.183,-99.183,-74.550,-74.550) Newfoundland Rivière du Sud ENVELOPE(70.132,70.132,-49.346,-49.346) Roberts Bay ENVELOPE(-99.064,-99.064,57.290,57.290) Saunders ENVELOPE(-45.316,-45.316,-60.700,-60.700) Tremblay ENVELOPE(-120.853,-120.853,55.783,55.783) |