Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome
Although common knowledge dictates that the lichen thallus is formed solely by a fungus (mycobiont) that develops a symbiotic relationship with an alga and/or cyanobacterium (photobiont), the non-photoautotrophic bacteria found in lichen microbiomes are increasingly regarded as integral components o...
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ftdryad:oai:v1.datadryad.org:10255/dryad.34223 2023-05-15T15:08:04+02:00 Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome Hodkinson, Brendan P. Gottel, Neil R. Schadt, Christopher W. Lutzoni, François Alaska North Carolina Costa Rica 2011-06-29T18:47:16Z http://hdl.handle.net/10255/dryad.34223 https://doi.org/10.5061/dryad.t99b1 unknown doi:10.5061/dryad.t99b1/1 doi:10.1111/j.1462-2920.2011.02560.x PMID:21906220 doi:10.5061/dryad.t99b1 Hodkinson BP, Gottel NR, Schadt CW, Lutzoni F (2011) Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome. Environmental Microbiology 14(1): 147-161. http://hdl.handle.net/10255/dryad.34223 16S rRNA gene 454 sequencing bacterial communities clone libraries lichens microbial ecology pyrosequencing Titanium series reagents Article 2011 ftdryad https://doi.org/10.5061/dryad.t99b1 https://doi.org/10.5061/dryad.t99b1/1 https://doi.org/10.1111/j.1462-2920.2011.02560.x 2020-01-01T14:53:56Z Although common knowledge dictates that the lichen thallus is formed solely by a fungus (mycobiont) that develops a symbiotic relationship with an alga and/or cyanobacterium (photobiont), the non-photoautotrophic bacteria found in lichen microbiomes are increasingly regarded as integral components of lichen thalli. For this study, comparative analyses were conducted on lichen-associated bacterial communities to test for effects of photobiont-types (i.e., green algal vs. cyanobacterial), mycobiont-types, and large-scale spatial distances (from tropical to arctic latitudes). Amplicons of the 16S (SSU) rRNA gene were examined using both Sanger sequencing of cloned fragments and barcoded pyrosequencing. Rhizobiales is typically the most abundant and taxonomically diverse order in lichen microbiomes; however, overall bacterial diversity in lichens is shown to be much higher than previously reported. Members of Acidobacteriaceae, Acetobacteraceae, Brucellaceae, and sequence group LAR1 are the most commonly found groups across the phylogenetically and geographically broad array of lichens examined here. Major bacterial community trends are significantly correlated with differences in large-scale geography, photobiont-type, and mycobiont-type. The lichen as a microcosm represents a structured, unique microbial habitat with greater ecological complexity and bacterial diversity than previously appreciated and can serve as a model system for studying larger ecological and evolutionary principles. Article in Journal/Newspaper Arctic Alaska Dryad Digital Repository (Duke University) Arctic |
institution |
Open Polar |
collection |
Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
16S rRNA gene 454 sequencing bacterial communities clone libraries lichens microbial ecology pyrosequencing Titanium series reagents |
spellingShingle |
16S rRNA gene 454 sequencing bacterial communities clone libraries lichens microbial ecology pyrosequencing Titanium series reagents Hodkinson, Brendan P. Gottel, Neil R. Schadt, Christopher W. Lutzoni, François Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome |
topic_facet |
16S rRNA gene 454 sequencing bacterial communities clone libraries lichens microbial ecology pyrosequencing Titanium series reagents |
description |
Although common knowledge dictates that the lichen thallus is formed solely by a fungus (mycobiont) that develops a symbiotic relationship with an alga and/or cyanobacterium (photobiont), the non-photoautotrophic bacteria found in lichen microbiomes are increasingly regarded as integral components of lichen thalli. For this study, comparative analyses were conducted on lichen-associated bacterial communities to test for effects of photobiont-types (i.e., green algal vs. cyanobacterial), mycobiont-types, and large-scale spatial distances (from tropical to arctic latitudes). Amplicons of the 16S (SSU) rRNA gene were examined using both Sanger sequencing of cloned fragments and barcoded pyrosequencing. Rhizobiales is typically the most abundant and taxonomically diverse order in lichen microbiomes; however, overall bacterial diversity in lichens is shown to be much higher than previously reported. Members of Acidobacteriaceae, Acetobacteraceae, Brucellaceae, and sequence group LAR1 are the most commonly found groups across the phylogenetically and geographically broad array of lichens examined here. Major bacterial community trends are significantly correlated with differences in large-scale geography, photobiont-type, and mycobiont-type. The lichen as a microcosm represents a structured, unique microbial habitat with greater ecological complexity and bacterial diversity than previously appreciated and can serve as a model system for studying larger ecological and evolutionary principles. |
format |
Article in Journal/Newspaper |
author |
Hodkinson, Brendan P. Gottel, Neil R. Schadt, Christopher W. Lutzoni, François |
author_facet |
Hodkinson, Brendan P. Gottel, Neil R. Schadt, Christopher W. Lutzoni, François |
author_sort |
Hodkinson, Brendan P. |
title |
Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome |
title_short |
Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome |
title_full |
Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome |
title_fullStr |
Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome |
title_full_unstemmed |
Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome |
title_sort |
data from: photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome |
publishDate |
2011 |
url |
http://hdl.handle.net/10255/dryad.34223 https://doi.org/10.5061/dryad.t99b1 |
op_coverage |
Alaska North Carolina Costa Rica |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Alaska |
genre_facet |
Arctic Alaska |
op_relation |
doi:10.5061/dryad.t99b1/1 doi:10.1111/j.1462-2920.2011.02560.x PMID:21906220 doi:10.5061/dryad.t99b1 Hodkinson BP, Gottel NR, Schadt CW, Lutzoni F (2011) Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome. Environmental Microbiology 14(1): 147-161. http://hdl.handle.net/10255/dryad.34223 |
op_doi |
https://doi.org/10.5061/dryad.t99b1 https://doi.org/10.5061/dryad.t99b1/1 https://doi.org/10.1111/j.1462-2920.2011.02560.x |
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