Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect
Rapid cold hardening (RCH) is a type of beneficial phenotypic plasticity that occurs on extremely short time scales (minutes to hours) to enhance insects' ability to cope with cold snaps and diurnal temperature fluctuations. RCH has a well-established role in extending lower lethal limits, but...
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ftdryad:oai:v1.datadryad.org:10255/dryad.215294 2023-05-15T14:02:40+02:00 Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect Teets, Nicholas M. Kawarasaki, Yuta Potts, Leslie J Benjamin, Philip N. Gantz, Josiah D. Denlinger, David L. Jr., Richard E. Lee Antarctica 2019-07-25T19:13:52Z http://hdl.handle.net/10255/dryad.215294 https://doi.org/10.5061/dryad.29p7ng2 unknown doi:10.5061/dryad.29p7ng2/1 doi:10.5061/dryad.29p7ng2 http://hdl.handle.net/10255/dryad.215294 acclimation freeze-tolerance heat shock proteins phenotypic plasticity stress Article 2019 ftdryad https://doi.org/10.5061/dryad.29p7ng2 https://doi.org/10.5061/dryad.29p7ng2/1 2020-01-01T16:27:56Z Rapid cold hardening (RCH) is a type of beneficial phenotypic plasticity that occurs on extremely short time scales (minutes to hours) to enhance insects' ability to cope with cold snaps and diurnal temperature fluctuations. RCH has a well-established role in extending lower lethal limits, but its ability to prevent sublethal cold injury has received less attention. The Antarctic midge, Belgica antarctica is Antarctica's only endemic insect and has a well-studied RCH response that extends freeze tolerance in laboratory conditions. However, the discriminating temperatures used in previous studies of RCH are far below those ever experienced in the field. Here, we tested the hypothesis that RCH protects against nonlethal freezing injury. Larvae of B. antarctica were exposed to either control (2&[deg]C), direct freezing (-9&[deg]C for 24 h), or RCH (-5&[deg]C for 2 h followed by -9&[deg]C for 24 h). All larvae survived both freezing treatments, but RCH larvae recovered more quickly from freezing stress and had significantly higher metabolic rates during recovery. RCH larvae also sustained less damage to fat body and midgut tissue and had lower expression of two heat shock protein transcripts (hsp60 and hsp90), which is consistent with RCH protecting against protein denaturation. The protection afforded by RCH resulted in energy savings; directly frozen larvae experienced a significant depletion in glycogen energy stores that was not observed in RCH larvae. Together, these results provide strong evidence that RCH protects against a variety of sublethal freezing injuries and allows insects to rapidly fine-tune their performance in thermally variable environments. Article in Journal/Newspaper Antarc* Antarctic Antarctic midge Antarctica Belgica antarctica Dryad Digital Repository (Duke University) Antarctic The Antarctic |
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Dryad Digital Repository (Duke University) |
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ftdryad |
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unknown |
topic |
acclimation freeze-tolerance heat shock proteins phenotypic plasticity stress |
spellingShingle |
acclimation freeze-tolerance heat shock proteins phenotypic plasticity stress Teets, Nicholas M. Kawarasaki, Yuta Potts, Leslie J Benjamin, Philip N. Gantz, Josiah D. Denlinger, David L. Jr., Richard E. Lee Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect |
topic_facet |
acclimation freeze-tolerance heat shock proteins phenotypic plasticity stress |
description |
Rapid cold hardening (RCH) is a type of beneficial phenotypic plasticity that occurs on extremely short time scales (minutes to hours) to enhance insects' ability to cope with cold snaps and diurnal temperature fluctuations. RCH has a well-established role in extending lower lethal limits, but its ability to prevent sublethal cold injury has received less attention. The Antarctic midge, Belgica antarctica is Antarctica's only endemic insect and has a well-studied RCH response that extends freeze tolerance in laboratory conditions. However, the discriminating temperatures used in previous studies of RCH are far below those ever experienced in the field. Here, we tested the hypothesis that RCH protects against nonlethal freezing injury. Larvae of B. antarctica were exposed to either control (2&[deg]C), direct freezing (-9&[deg]C for 24 h), or RCH (-5&[deg]C for 2 h followed by -9&[deg]C for 24 h). All larvae survived both freezing treatments, but RCH larvae recovered more quickly from freezing stress and had significantly higher metabolic rates during recovery. RCH larvae also sustained less damage to fat body and midgut tissue and had lower expression of two heat shock protein transcripts (hsp60 and hsp90), which is consistent with RCH protecting against protein denaturation. The protection afforded by RCH resulted in energy savings; directly frozen larvae experienced a significant depletion in glycogen energy stores that was not observed in RCH larvae. Together, these results provide strong evidence that RCH protects against a variety of sublethal freezing injuries and allows insects to rapidly fine-tune their performance in thermally variable environments. |
format |
Article in Journal/Newspaper |
author |
Teets, Nicholas M. Kawarasaki, Yuta Potts, Leslie J Benjamin, Philip N. Gantz, Josiah D. Denlinger, David L. Jr., Richard E. Lee |
author_facet |
Teets, Nicholas M. Kawarasaki, Yuta Potts, Leslie J Benjamin, Philip N. Gantz, Josiah D. Denlinger, David L. Jr., Richard E. Lee |
author_sort |
Teets, Nicholas M. |
title |
Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect |
title_short |
Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect |
title_full |
Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect |
title_fullStr |
Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect |
title_full_unstemmed |
Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect |
title_sort |
data from: rapid cold hardening protects against sublethal freezing injury in an antarctic insect |
publishDate |
2019 |
url |
http://hdl.handle.net/10255/dryad.215294 https://doi.org/10.5061/dryad.29p7ng2 |
op_coverage |
Antarctica |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic midge Antarctica Belgica antarctica |
genre_facet |
Antarc* Antarctic Antarctic midge Antarctica Belgica antarctica |
op_relation |
doi:10.5061/dryad.29p7ng2/1 doi:10.5061/dryad.29p7ng2 http://hdl.handle.net/10255/dryad.215294 |
op_doi |
https://doi.org/10.5061/dryad.29p7ng2 https://doi.org/10.5061/dryad.29p7ng2/1 |
_version_ |
1766273014515630080 |