Fasta filtering and DNAm scoring pipeline
This file takes in maxee filtered and dereplicated sample fasta files. Samples are then checked for the correct sequence site “CCGGG” or “GGG” at the beginning of each sequence. Samples are trimmed to be the same length and dereplicated using USEARCH10. A database of each set of unique sequences of...
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2018
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Online Access: | http://hdl.handle.net/10255/dryad.197025 https://doi.org/10.5061/dryad.n4h3672/3 |
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ftdryad:oai:v1.datadryad.org:10255/dryad.197025 2023-05-15T16:17:22+02:00 Fasta filtering and DNAm scoring pipeline De Paoli-Iseppi, Ricardo Deagle, Bruce Polanowski, Andrea McMahon, Clive Dickinson, Joanne Hindell, Mark Jarman, Simon Flinders Island Fisher Island Tasmania 2018-12-18T18:04:02Z 18592 http://hdl.handle.net/10255/dryad.197025 https://doi.org/10.5061/dryad.n4h3672/3 unknown doi:10.5061/dryad.n4h3672 doi:10.5061/dryad.n4h3672/3 http://hdl.handle.net/10255/dryad.197025 http://creativecommons.org/publicdomain/zero/1.0/ CC0 PDM Age Birds DNA methylation Epigenetics DREAM Dataset 2018 ftdryad https://doi.org/10.5061/dryad.n4h3672/3 https://doi.org/10.5061/dryad.n4h3672 2020-01-01T16:18:22Z This file takes in maxee filtered and dereplicated sample fasta files. Samples are then checked for the correct sequence site “CCGGG” or “GGG” at the beginning of each sequence. Samples are trimmed to be the same length and dereplicated using USEARCH10. A database of each set of unique sequences of CCGGG or GGG starting sites is created e.g. a methylated and unmethylated database. Samples are compared against these two databases and counts recorded to generate scores between 0-1. DNAm scores can then be further filtered on read depth, standard deviation or output as needed. Dataset Fisher Island Dryad Digital Repository (Duke University) Flinders ENVELOPE(-66.667,-66.667,-69.267,-69.267) |
institution |
Open Polar |
collection |
Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
Age Birds DNA methylation Epigenetics DREAM |
spellingShingle |
Age Birds DNA methylation Epigenetics DREAM De Paoli-Iseppi, Ricardo Deagle, Bruce Polanowski, Andrea McMahon, Clive Dickinson, Joanne Hindell, Mark Jarman, Simon Fasta filtering and DNAm scoring pipeline |
topic_facet |
Age Birds DNA methylation Epigenetics DREAM |
description |
This file takes in maxee filtered and dereplicated sample fasta files. Samples are then checked for the correct sequence site “CCGGG” or “GGG” at the beginning of each sequence. Samples are trimmed to be the same length and dereplicated using USEARCH10. A database of each set of unique sequences of CCGGG or GGG starting sites is created e.g. a methylated and unmethylated database. Samples are compared against these two databases and counts recorded to generate scores between 0-1. DNAm scores can then be further filtered on read depth, standard deviation or output as needed. |
format |
Dataset |
author |
De Paoli-Iseppi, Ricardo Deagle, Bruce Polanowski, Andrea McMahon, Clive Dickinson, Joanne Hindell, Mark Jarman, Simon |
author_facet |
De Paoli-Iseppi, Ricardo Deagle, Bruce Polanowski, Andrea McMahon, Clive Dickinson, Joanne Hindell, Mark Jarman, Simon |
author_sort |
De Paoli-Iseppi, Ricardo |
title |
Fasta filtering and DNAm scoring pipeline |
title_short |
Fasta filtering and DNAm scoring pipeline |
title_full |
Fasta filtering and DNAm scoring pipeline |
title_fullStr |
Fasta filtering and DNAm scoring pipeline |
title_full_unstemmed |
Fasta filtering and DNAm scoring pipeline |
title_sort |
fasta filtering and dnam scoring pipeline |
publishDate |
2018 |
url |
http://hdl.handle.net/10255/dryad.197025 https://doi.org/10.5061/dryad.n4h3672/3 |
op_coverage |
Flinders Island Fisher Island Tasmania |
long_lat |
ENVELOPE(-66.667,-66.667,-69.267,-69.267) |
geographic |
Flinders |
geographic_facet |
Flinders |
genre |
Fisher Island |
genre_facet |
Fisher Island |
op_relation |
doi:10.5061/dryad.n4h3672 doi:10.5061/dryad.n4h3672/3 http://hdl.handle.net/10255/dryad.197025 |
op_rights |
http://creativecommons.org/publicdomain/zero/1.0/ |
op_rightsnorm |
CC0 PDM |
op_doi |
https://doi.org/10.5061/dryad.n4h3672/3 https://doi.org/10.5061/dryad.n4h3672 |
_version_ |
1766003217434411008 |