Data from: No support for cryptic choice by ovarian fluid in an external fertilizer
Whether the ovarian fluid represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of ovarian fluid on sperm swimming behaviour - the Arctic charr (Salvelinus alpinus). We physically separate...
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ftdryad:oai:v1.datadryad.org:10255/dryad.194797 2023-05-15T14:30:10+02:00 Data from: No support for cryptic choice by ovarian fluid in an external fertilizer Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar 2018-11-10T13:01:12Z http://hdl.handle.net/10255/dryad.194797 https://doi.org/10.5061/dryad.7kd12bg unknown doi:10.5061/dryad.7kd12bg/1 doi:10.5061/dryad.7kd12bg/2 doi:10.1002/ece3.4628 doi:10.5061/dryad.7kd12bg Kleppe SA, Nordeide JT, Rudolfsen G, Figenschou L, Larsen B, Reiss K, Folstad I (2018) No support for cryptic choice by ovarian fluid in an external fertilizer. Ecology and Evolution 8(23): 11763-11774. http://hdl.handle.net/10255/dryad.194797 Article 2018 ftdryad https://doi.org/10.5061/dryad.7kd12bg https://doi.org/10.5061/dryad.7kd12bg/1 https://doi.org/10.5061/dryad.7kd12bg/2 https://doi.org/10.1002/ece3.4628 2020-01-01T16:17:12Z Whether the ovarian fluid represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of ovarian fluid on sperm swimming behaviour - the Arctic charr (Salvelinus alpinus). We physically separated the ovarian fluid from the eggs of reproductively active females and reintroduced either their own ovarian fluid or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split-brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual ovarian fluids. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of ovarian fluid treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that ovarian fluid plays an important role as medium for cryptic female choice in charr. Power-analyses revealed that our sample size is large enough to detect medium sized changes in relative paternity (medium-sized effect-sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about ovarian fluid’s potential influence on paternity under sperm competition - even in charr. Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Dryad Digital Repository (Duke University) Arctic |
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Dryad Digital Repository (Duke University) |
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ftdryad |
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unknown |
description |
Whether the ovarian fluid represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of ovarian fluid on sperm swimming behaviour - the Arctic charr (Salvelinus alpinus). We physically separated the ovarian fluid from the eggs of reproductively active females and reintroduced either their own ovarian fluid or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split-brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual ovarian fluids. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of ovarian fluid treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that ovarian fluid plays an important role as medium for cryptic female choice in charr. Power-analyses revealed that our sample size is large enough to detect medium sized changes in relative paternity (medium-sized effect-sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about ovarian fluid’s potential influence on paternity under sperm competition - even in charr. |
format |
Article in Journal/Newspaper |
author |
Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar |
spellingShingle |
Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar Data from: No support for cryptic choice by ovarian fluid in an external fertilizer |
author_facet |
Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar |
author_sort |
Kleppe, Snøfrid April |
title |
Data from: No support for cryptic choice by ovarian fluid in an external fertilizer |
title_short |
Data from: No support for cryptic choice by ovarian fluid in an external fertilizer |
title_full |
Data from: No support for cryptic choice by ovarian fluid in an external fertilizer |
title_fullStr |
Data from: No support for cryptic choice by ovarian fluid in an external fertilizer |
title_full_unstemmed |
Data from: No support for cryptic choice by ovarian fluid in an external fertilizer |
title_sort |
data from: no support for cryptic choice by ovarian fluid in an external fertilizer |
publishDate |
2018 |
url |
http://hdl.handle.net/10255/dryad.194797 https://doi.org/10.5061/dryad.7kd12bg |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_relation |
doi:10.5061/dryad.7kd12bg/1 doi:10.5061/dryad.7kd12bg/2 doi:10.1002/ece3.4628 doi:10.5061/dryad.7kd12bg Kleppe SA, Nordeide JT, Rudolfsen G, Figenschou L, Larsen B, Reiss K, Folstad I (2018) No support for cryptic choice by ovarian fluid in an external fertilizer. Ecology and Evolution 8(23): 11763-11774. http://hdl.handle.net/10255/dryad.194797 |
op_doi |
https://doi.org/10.5061/dryad.7kd12bg https://doi.org/10.5061/dryad.7kd12bg/1 https://doi.org/10.5061/dryad.7kd12bg/2 https://doi.org/10.1002/ece3.4628 |
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1766304072100478976 |