Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly und...
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ftdryad:oai:v1.datadryad.org:10255/dryad.194241 2023-05-15T15:58:01+02:00 Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. 2018-10-25T13:58:03Z http://hdl.handle.net/10255/dryad.194241 https://doi.org/10.5061/dryad.kn45n49 unknown doi:10.5061/dryad.kn45n49/1 doi:10.5061/dryad.kn45n49/2 doi:10.5061/dryad.kn45n49/3 doi:10.5061/dryad.kn45n49/4 doi:10.5061/dryad.kn45n49/5 doi:10.5061/dryad.kn45n49/6 doi:10.5061/dryad.kn45n49/7 doi:10.5061/dryad.kn45n49/8 doi:10.5061/dryad.kn45n49/9 doi:10.5061/dryad.kn45n49/10 doi:10.5061/dryad.kn45n49/11 doi:10.5061/dryad.kn45n49/12 doi:10.1098/rspb.2018.1676 doi:10.5061/dryad.kn45n49 Sillanpää JK, Sundh H, Sundell KS (2018) Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas. Proceedings of the Royal Society B 285(1891): 20181676. http://hdl.handle.net/10255/dryad.194241 biomineralisation calcification Ussing chamber technique Ca2+ transport Ca2+ kinetics Article 2018 ftdryad https://doi.org/10.5061/dryad.kn45n49 https://doi.org/10.5061/dryad.kn45n49/1 https://doi.org/10.5061/dryad.kn45n49/2 https://doi.org/10.5061/dryad.kn45n49/3 https://doi.org/10.5061/dryad.kn45n49/4 https://doi.org/10.5061/dryad.kn45n49/5 https 2020-01-01T16:16:53Z Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a Km of 6.2 mM and a Vmax of 3.3 nM min−1. The transcellular transfer of Ca2+ accounts for approximately 60% of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca2+-channels, plasma-membrane Ca2+-ATPase and Na+/Ca2+-exchanger all participate in the transcellular Ca2+ transfer across the OME and a model for this Ca2+ transfer is presented and discussed. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Dryad Digital Repository (Duke University) Pacific |
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Open Polar |
collection |
Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
biomineralisation calcification Ussing chamber technique Ca2+ transport Ca2+ kinetics |
spellingShingle |
biomineralisation calcification Ussing chamber technique Ca2+ transport Ca2+ kinetics Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
topic_facet |
biomineralisation calcification Ussing chamber technique Ca2+ transport Ca2+ kinetics |
description |
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a Km of 6.2 mM and a Vmax of 3.3 nM min−1. The transcellular transfer of Ca2+ accounts for approximately 60% of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca2+-channels, plasma-membrane Ca2+-ATPase and Na+/Ca2+-exchanger all participate in the transcellular Ca2+ transfer across the OME and a model for this Ca2+ transfer is presented and discussed. |
format |
Article in Journal/Newspaper |
author |
Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. |
author_facet |
Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. |
author_sort |
Sillanpää, J. Kirsikka |
title |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_short |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_full |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_sort |
data from: calcium transfer across the outer mantle epithelium in the pacific oyster, crassostrea gigas |
publishDate |
2018 |
url |
http://hdl.handle.net/10255/dryad.194241 https://doi.org/10.5061/dryad.kn45n49 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.5061/dryad.kn45n49/1 doi:10.5061/dryad.kn45n49/2 doi:10.5061/dryad.kn45n49/3 doi:10.5061/dryad.kn45n49/4 doi:10.5061/dryad.kn45n49/5 doi:10.5061/dryad.kn45n49/6 doi:10.5061/dryad.kn45n49/7 doi:10.5061/dryad.kn45n49/8 doi:10.5061/dryad.kn45n49/9 doi:10.5061/dryad.kn45n49/10 doi:10.5061/dryad.kn45n49/11 doi:10.5061/dryad.kn45n49/12 doi:10.1098/rspb.2018.1676 doi:10.5061/dryad.kn45n49 Sillanpää JK, Sundh H, Sundell KS (2018) Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas. Proceedings of the Royal Society B 285(1891): 20181676. http://hdl.handle.net/10255/dryad.194241 |
op_doi |
https://doi.org/10.5061/dryad.kn45n49 https://doi.org/10.5061/dryad.kn45n49/1 https://doi.org/10.5061/dryad.kn45n49/2 https://doi.org/10.5061/dryad.kn45n49/3 https://doi.org/10.5061/dryad.kn45n49/4 https://doi.org/10.5061/dryad.kn45n49/5 https |
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