Data from: Identification of genomic regions associated with sex in Pacific halibut
Understanding and identifying the genetic mechanisms responsible for sex-determination are important for species management, particularly in exploited fishes where sex biased harvest could have implications on population dynamics and long-term persistence. The Pacific halibut (Hippoglossus stenolepi...
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ftdryad:oai:v1.datadryad.org:10255/dryad.162483 2023-05-15T18:15:52+02:00 Data from: Identification of genomic regions associated with sex in Pacific halibut Drinan, Daniel P. Loher, Timothy Hauser, Lorenz 2017-11-06T15:10:06Z http://hdl.handle.net/10255/dryad.162483 https://doi.org/10.5061/dryad.ff072 unknown doi:10.5061/dryad.ff072/1 doi:10.5061/dryad.ff072/2 doi:10.5061/dryad.ff072/3 doi:10.5061/dryad.ff072/4 doi:10.1093/jhered/esx102 doi:10.5061/dryad.ff072 Drinan DP, Loher T, Hauser L (2018) Identification of genomic regions associated with sex in Pacific halibut. Journal of Heredity 109(3): 326-332. http://hdl.handle.net/10255/dryad.162483 Pacific halibut sex identification fisheries RAD comparative genomics sex determination Genomics and gene mapping Conservation genetics and biodiversity Article 2017 ftdryad https://doi.org/10.5061/dryad.ff072 https://doi.org/10.5061/dryad.ff072/1 https://doi.org/10.5061/dryad.ff072/2 https://doi.org/10.5061/dryad.ff072/3 https://doi.org/10.5061/dryad.ff072/4 https://doi.org/10.1093/jhered/esx102 2020-01-01T15:59:42Z Understanding and identifying the genetic mechanisms responsible for sex-determination are important for species management, particularly in exploited fishes where sex biased harvest could have implications on population dynamics and long-term persistence. The Pacific halibut (Hippoglossus stenolepis) supports important fisheries in the North Pacific Ocean. The proportion of each sex in the annual harvest is currently estimated using growth curves, but genetic techniques may provide a more accurate method. We used restriction-site associated DNA (RAD) sequencing to identify RAD-tags that were linked to genetic sex, based on differentiation (FST) between the sexes. Identified RAD-tags were aligned to the Atlantic halibut (Hippoglossus hippoglossus) linkage map, the turbot (Scophthalmus maximus) genome, and the half-smooth tongue sole (Cynoglossus semilaevis) genome to identify genomic regions that may be involved in sex determination. In total, 56 RAD-tags (70 single nucleotide polymorphisms) were linked to sex, and three RAD-tags were identified in only females. Sex-linked loci aligned to three linkage groups in the Atlantic halibut (LG07: 7 loci, LG15: 1 locus, and LG24: 1 locus), three chromosomes in the turbot (LG12: 13 loci, LG01: 1 locus, and LG05: 1 locus), and one chromosome in the half-smooth tongue sole (ChrZ: 9 loci). Results add support to the hypothesis that Pacific halibut genetic sex is determined in a ZW system. Two sex-linked loci were further developed into sex identification assays, and their efficacy was tested on individuals that had been morphologically sexed. The accuracy of each assay on its own was 97.5% compared to morphological sex. Article in Journal/Newspaper Scophthalmus maximus Turbot Dryad Digital Repository (Duke University) Pacific |
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Open Polar |
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Dryad Digital Repository (Duke University) |
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ftdryad |
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unknown |
topic |
Pacific halibut sex identification fisheries RAD comparative genomics sex determination Genomics and gene mapping Conservation genetics and biodiversity |
spellingShingle |
Pacific halibut sex identification fisheries RAD comparative genomics sex determination Genomics and gene mapping Conservation genetics and biodiversity Drinan, Daniel P. Loher, Timothy Hauser, Lorenz Data from: Identification of genomic regions associated with sex in Pacific halibut |
topic_facet |
Pacific halibut sex identification fisheries RAD comparative genomics sex determination Genomics and gene mapping Conservation genetics and biodiversity |
description |
Understanding and identifying the genetic mechanisms responsible for sex-determination are important for species management, particularly in exploited fishes where sex biased harvest could have implications on population dynamics and long-term persistence. The Pacific halibut (Hippoglossus stenolepis) supports important fisheries in the North Pacific Ocean. The proportion of each sex in the annual harvest is currently estimated using growth curves, but genetic techniques may provide a more accurate method. We used restriction-site associated DNA (RAD) sequencing to identify RAD-tags that were linked to genetic sex, based on differentiation (FST) between the sexes. Identified RAD-tags were aligned to the Atlantic halibut (Hippoglossus hippoglossus) linkage map, the turbot (Scophthalmus maximus) genome, and the half-smooth tongue sole (Cynoglossus semilaevis) genome to identify genomic regions that may be involved in sex determination. In total, 56 RAD-tags (70 single nucleotide polymorphisms) were linked to sex, and three RAD-tags were identified in only females. Sex-linked loci aligned to three linkage groups in the Atlantic halibut (LG07: 7 loci, LG15: 1 locus, and LG24: 1 locus), three chromosomes in the turbot (LG12: 13 loci, LG01: 1 locus, and LG05: 1 locus), and one chromosome in the half-smooth tongue sole (ChrZ: 9 loci). Results add support to the hypothesis that Pacific halibut genetic sex is determined in a ZW system. Two sex-linked loci were further developed into sex identification assays, and their efficacy was tested on individuals that had been morphologically sexed. The accuracy of each assay on its own was 97.5% compared to morphological sex. |
format |
Article in Journal/Newspaper |
author |
Drinan, Daniel P. Loher, Timothy Hauser, Lorenz |
author_facet |
Drinan, Daniel P. Loher, Timothy Hauser, Lorenz |
author_sort |
Drinan, Daniel P. |
title |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_short |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_full |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_fullStr |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_full_unstemmed |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_sort |
data from: identification of genomic regions associated with sex in pacific halibut |
publishDate |
2017 |
url |
http://hdl.handle.net/10255/dryad.162483 https://doi.org/10.5061/dryad.ff072 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
doi:10.5061/dryad.ff072/1 doi:10.5061/dryad.ff072/2 doi:10.5061/dryad.ff072/3 doi:10.5061/dryad.ff072/4 doi:10.1093/jhered/esx102 doi:10.5061/dryad.ff072 Drinan DP, Loher T, Hauser L (2018) Identification of genomic regions associated with sex in Pacific halibut. Journal of Heredity 109(3): 326-332. http://hdl.handle.net/10255/dryad.162483 |
op_doi |
https://doi.org/10.5061/dryad.ff072 https://doi.org/10.5061/dryad.ff072/1 https://doi.org/10.5061/dryad.ff072/2 https://doi.org/10.5061/dryad.ff072/3 https://doi.org/10.5061/dryad.ff072/4 https://doi.org/10.1093/jhered/esx102 |
_version_ |
1766189109075771392 |