Plant tree (all) Mar 11

• Main Authors: Gibson, Daniel, Adamowicz, Sarah, Jacobs, Shoshanah R., Smith, M Alex
• Format: Dataset
• Language: unknown
• Published: 2018
• Subjects: host specificity; phylogenetic clustering; aphids; Arctic; Hollingsworth
• Online Access: http://hdl.handle.net/10255/dryad.151870; https://doi.org/10.5061/dryad.5ds70/3

To create a phylogeny of locally occurring plants in Churchill, we used the existing DNA barcode library derived from the majority of the vascular plants of Churchill (Kuzmina et al. 2012) (Supp. Appendix 1). Each plant species targeted in our study had three DNA barcode gene regions (ITS2, rbcLa, and matK; Hollingsworth et al. 2009) represented with the exception of Larix laricina, which lacked a matK sequence. For each plant species, and each gene region, the highest quality sequence (longest sequence containing the lowest number of ambiguous bases) was selected. The three genes were concatenated using FaBox (Villesen 2007), and the resulting contiguous sequence was used to create a Maximum-Likelihood tree (500 bootstrap replicates) in MEGA6.