Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus)
The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is requi...
Main Authors: | , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2017
|
Subjects: | |
Online Access: | http://hdl.handle.net/10255/dryad.143000 https://doi.org/10.5061/dryad.d4368 |
id |
ftdryad:oai:v1.datadryad.org:10255/dryad.143000 |
---|---|
record_format |
openpolar |
spelling |
ftdryad:oai:v1.datadryad.org:10255/dryad.143000 2023-05-15T17:07:49+02:00 Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique 2017-04-25T15:09:27Z http://hdl.handle.net/10255/dryad.143000 https://doi.org/10.5061/dryad.d4368 unknown doi:10.5061/dryad.d4368/1 doi:10.1002/ece3.2943 doi:10.5061/dryad.d4368 Beugin M, Letty J, Kaerle C, Guitton J, Muselet L, Queney G, Pontier D (2017) A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus ) and the mountain hare (Lepus timidus ). Ecology and Evolution 7(11): 3931-3939. 2045-7758 http://hdl.handle.net/10255/dryad.143000 Article 2017 ftdryad https://doi.org/10.5061/dryad.d4368 https://doi.org/10.5061/dryad.d4368/1 https://doi.org/10.1002/ece3.2943 2020-01-01T15:49:19Z The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is required to establish optimal management actions. This is why genetic tools have been more and more integrated in wildlife management practices. However, the markers proposed are often amplified in small multiplexes when larger ones could allow to better cope with the small quantities of DNA obtained with non-invasive sampling methods. Here, we propose a unique multiplex of 12 autosomal microsatellite markers for the study of two hare species that exist in sympatry in some areas in Europe and are hunted notably in France: the brown hare Lepus europaeus and the mountain hare L. timidus. We tested 17 markers previously used in these two species or other lagomorph species, from which 12 were included in this single multiplex. Diversity was between 4 and 30 alleles per locus totalling 126 alleles and we showed that these markers possess appropriate genetic resolution for individual and species identification for the populations under study. This multiplex panel represents the largest number of microsatellites amplified in one reaction proposed for these two hare species and provides a cost-effective and valuable tool for further hybridization studies and the management of hares. Article in Journal/Newspaper Lepus timidus mountain hare Dryad Digital Repository (Duke University) |
institution |
Open Polar |
collection |
Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
description |
The management of hunted species is challenging, as it must conciliate the conservation of species and their sustainable exploitation. Non-genetic tools are widely used in this context but they may present limitations notably when species can hybridize or when large-scale spatial monitoring is required to establish optimal management actions. This is why genetic tools have been more and more integrated in wildlife management practices. However, the markers proposed are often amplified in small multiplexes when larger ones could allow to better cope with the small quantities of DNA obtained with non-invasive sampling methods. Here, we propose a unique multiplex of 12 autosomal microsatellite markers for the study of two hare species that exist in sympatry in some areas in Europe and are hunted notably in France: the brown hare Lepus europaeus and the mountain hare L. timidus. We tested 17 markers previously used in these two species or other lagomorph species, from which 12 were included in this single multiplex. Diversity was between 4 and 30 alleles per locus totalling 126 alleles and we showed that these markers possess appropriate genetic resolution for individual and species identification for the populations under study. This multiplex panel represents the largest number of microsatellites amplified in one reaction proposed for these two hare species and provides a cost-effective and valuable tool for further hybridization studies and the management of hares. |
format |
Article in Journal/Newspaper |
author |
Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique |
spellingShingle |
Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
author_facet |
Beugin, Marie-Pauline Letty, Jérôme Kaerle, Cécile Guitton, Jean-Sébastien Muselet, Lina Queney, Guillaume Pontier, Dominique |
author_sort |
Beugin, Marie-Pauline |
title |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_short |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_full |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_fullStr |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_full_unstemmed |
Data from: A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus) and the mountain hare (Lepus timidus) |
title_sort |
data from: a single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (lepus europaeus) and the mountain hare (lepus timidus) |
publishDate |
2017 |
url |
http://hdl.handle.net/10255/dryad.143000 https://doi.org/10.5061/dryad.d4368 |
genre |
Lepus timidus mountain hare |
genre_facet |
Lepus timidus mountain hare |
op_relation |
doi:10.5061/dryad.d4368/1 doi:10.1002/ece3.2943 doi:10.5061/dryad.d4368 Beugin M, Letty J, Kaerle C, Guitton J, Muselet L, Queney G, Pontier D (2017) A single multiplex of twelve microsatellite markers for the simultaneous study of the brown hare (Lepus europaeus ) and the mountain hare (Lepus timidus ). Ecology and Evolution 7(11): 3931-3939. 2045-7758 http://hdl.handle.net/10255/dryad.143000 |
op_doi |
https://doi.org/10.5061/dryad.d4368 https://doi.org/10.5061/dryad.d4368/1 https://doi.org/10.1002/ece3.2943 |
_version_ |
1766063327926026240 |