Data from: Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes
Remote polar and deepwater fish faunas are under pressure from ongoing climate change and increasing fishing effort. However, these fish communities are difficult to monitor for logistic and financial reasons. Currently, monitoring of marine fishes largely relies on invasive techniques such as botto...
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ftdryad:oai:v1.datadryad.org:10255/dryad.127118 2023-05-15T16:27:45+02:00 Data from: Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes Thomsen, Philip Francis Møller, Peter Rask Sigsgaard, Eva Egelyng Knudsen, Steen Wilhelm Jørgensen, Ole Ankjær Willerslev, Eske 2016-11-17T20:55:29Z http://hdl.handle.net/10255/dryad.127118 https://doi.org/10.5061/dryad.ch576 unknown doi:10.5061/dryad.ch576/1 doi:10.1371/journal.pone.0165252 doi:10.5061/dryad.ch576 Thomsen PF, Møller PR, Sigsgaard EE, Knudsen SW, Jørgensen OA, Willerslev E (2016) Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes. PLOS ONE 11(11): e0165252. 1932-6203 http://hdl.handle.net/10255/dryad.127118 Article 2016 ftdryad https://doi.org/10.5061/dryad.ch576 https://doi.org/10.5061/dryad.ch576/1 https://doi.org/10.1371/journal.pone.0165252 2020-01-01T15:40:54Z Remote polar and deepwater fish faunas are under pressure from ongoing climate change and increasing fishing effort. However, these fish communities are difficult to monitor for logistic and financial reasons. Currently, monitoring of marine fishes largely relies on invasive techniques such as bottom trawling, and on official reporting of global catches, which can be unreliable. Thus, there is need for alternative and non-invasive techniques for qualitative and quantitative oceanic fish surveys. Here we report environmental DNA (eDNA) metabarcoding of seawater samples from continental slope depths in Southwest Greenland. We collected seawater samples at depths of 188–918 m and compared seawater eDNA to catch data from trawling. We used Illumina sequencing of PCR products to demonstrate that eDNA reads show equivalence to fishing catch data obtained from trawling. Twenty-six families were found with both trawling and eDNA, while three families were found only with eDNA and two families were found only with trawling. Key commercial fish species for Greenland were the most abundant species in both eDNA reads and biomass catch, and interpolation of eDNA abundances between sampling sites showed good correspondence with catch sizes. Environmental DNA sequence reads from the fish assemblages correlated with biomass and abundance data obtained from trawling. Interestingly, the Greenland shark (Somniosus microcephalus) showed high abundance of eDNA reads despite only a single specimen being caught, demonstrating the relevance of the eDNA approach for large species that can probably avoid bottom trawls in most cases. Quantitative detection of marine fish using eDNA remains to be tested further to ascertain whether this technique is able to yield credible results for routine application in fisheries. Nevertheless, our study demonstrates that eDNA reads can be used as a qualitative and quantitative proxy for marine fish assemblages in deepwater oceanic habitats. This relates directly to applied fisheries as well as to monitoring effects of ongoing climate change on marine biodiversity—especially in polar ecosystems. Article in Journal/Newspaper Greenland Somniosus microcephalus Subarctic Dryad Digital Repository (Duke University) Greenland |
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Dryad Digital Repository (Duke University) |
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Remote polar and deepwater fish faunas are under pressure from ongoing climate change and increasing fishing effort. However, these fish communities are difficult to monitor for logistic and financial reasons. Currently, monitoring of marine fishes largely relies on invasive techniques such as bottom trawling, and on official reporting of global catches, which can be unreliable. Thus, there is need for alternative and non-invasive techniques for qualitative and quantitative oceanic fish surveys. Here we report environmental DNA (eDNA) metabarcoding of seawater samples from continental slope depths in Southwest Greenland. We collected seawater samples at depths of 188–918 m and compared seawater eDNA to catch data from trawling. We used Illumina sequencing of PCR products to demonstrate that eDNA reads show equivalence to fishing catch data obtained from trawling. Twenty-six families were found with both trawling and eDNA, while three families were found only with eDNA and two families were found only with trawling. Key commercial fish species for Greenland were the most abundant species in both eDNA reads and biomass catch, and interpolation of eDNA abundances between sampling sites showed good correspondence with catch sizes. Environmental DNA sequence reads from the fish assemblages correlated with biomass and abundance data obtained from trawling. Interestingly, the Greenland shark (Somniosus microcephalus) showed high abundance of eDNA reads despite only a single specimen being caught, demonstrating the relevance of the eDNA approach for large species that can probably avoid bottom trawls in most cases. Quantitative detection of marine fish using eDNA remains to be tested further to ascertain whether this technique is able to yield credible results for routine application in fisheries. Nevertheless, our study demonstrates that eDNA reads can be used as a qualitative and quantitative proxy for marine fish assemblages in deepwater oceanic habitats. This relates directly to applied fisheries as well as to monitoring effects of ongoing climate change on marine biodiversity—especially in polar ecosystems. |
format |
Article in Journal/Newspaper |
author |
Thomsen, Philip Francis Møller, Peter Rask Sigsgaard, Eva Egelyng Knudsen, Steen Wilhelm Jørgensen, Ole Ankjær Willerslev, Eske |
spellingShingle |
Thomsen, Philip Francis Møller, Peter Rask Sigsgaard, Eva Egelyng Knudsen, Steen Wilhelm Jørgensen, Ole Ankjær Willerslev, Eske Data from: Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes |
author_facet |
Thomsen, Philip Francis Møller, Peter Rask Sigsgaard, Eva Egelyng Knudsen, Steen Wilhelm Jørgensen, Ole Ankjær Willerslev, Eske |
author_sort |
Thomsen, Philip Francis |
title |
Data from: Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes |
title_short |
Data from: Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes |
title_full |
Data from: Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes |
title_fullStr |
Data from: Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes |
title_full_unstemmed |
Data from: Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes |
title_sort |
data from: environmental dna from seawater samples correlate with trawl catches of subarctic, deepwater fishes |
publishDate |
2016 |
url |
http://hdl.handle.net/10255/dryad.127118 https://doi.org/10.5061/dryad.ch576 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Somniosus microcephalus Subarctic |
genre_facet |
Greenland Somniosus microcephalus Subarctic |
op_relation |
doi:10.5061/dryad.ch576/1 doi:10.1371/journal.pone.0165252 doi:10.5061/dryad.ch576 Thomsen PF, Møller PR, Sigsgaard EE, Knudsen SW, Jørgensen OA, Willerslev E (2016) Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes. PLOS ONE 11(11): e0165252. 1932-6203 http://hdl.handle.net/10255/dryad.127118 |
op_doi |
https://doi.org/10.5061/dryad.ch576 https://doi.org/10.5061/dryad.ch576/1 https://doi.org/10.1371/journal.pone.0165252 |
_version_ |
1766017259706253312 |