Data from: The dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts
Supplementation of nutrients by symbionts enables consumers to thrive on resources that might otherwise be insufficient to meet nutritional demands. Such nutritional subsidies by intracellular symbionts has been well studied; however, supplementation of de novo synthesized nutrients to hosts by extr...
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Online Access: | http://hdl.handle.net/10255/dryad.118271 https://doi.org/10.5061/dryad.6f798 |
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ftdryad:oai:v1.datadryad.org:10255/dryad.118271 2023-05-15T14:26:51+02:00 Data from: The dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts Larsen, Thomas Ventura, Marc Maraldo, Kristine Triadó-Margarit, Xavier Casamayor, Emilio O. Wang, Yiming V. Andersen, Nils O'Brien, Diane M. Alaska Denmark 2016-07-05T15:04:19Z http://hdl.handle.net/10255/dryad.118271 https://doi.org/10.5061/dryad.6f798 unknown doi:10.5061/dryad.6f798/1 doi:10.1111/1365-2656.12563 PMID:27322934 doi:10.5061/dryad.6f798 Larsen T, Ventura M, Maraldo K, Triadó-Margarit X, Casamayor EO, Wang YV, Andersen N, O'Brien DM (2016) The dominant detritus-feeding invertebrate in Arctic peat soils derives its essential amino acids from gut symbionts. Journal of Animal Ecology 85(5): 1275-1285. 0021-8790 http://hdl.handle.net/10255/dryad.118271 Stable carbon isotopes amino acids Article 2016 ftdryad https://doi.org/10.5061/dryad.6f798 https://doi.org/10.5061/dryad.6f798/1 https://doi.org/10.1111/1365-2656.12563 2020-01-01T15:36:11Z Supplementation of nutrients by symbionts enables consumers to thrive on resources that might otherwise be insufficient to meet nutritional demands. Such nutritional subsidies by intracellular symbionts has been well studied; however, supplementation of de novo synthesized nutrients to hosts by extracellular gut symbionts is poorly documented, especially for generalists with relatively undifferentiated intestinal tracts. Although gut symbionts facilitate degradation of resources that would otherwise remain inaccessible to the host, such digestive actions alone cannot make up for dietary insufficiencies of macronutrients such as essential amino acids (EAA). Documenting whether gut symbionts also function as partners for symbiotic EAA supplementation is important because the question of how some detritivores are able to subsist on nutritionally insufficient diets has remained unresolved. To answer this poorly-understood nutritional aspect of symbiont-host interactions, we studied the enchytraeid worm, a bulk soil feeder that thrives in arctic peatlands. In a combined field and laboratory study, we employed stable isotope fingerprinting of amino acids to identify the biosynthetic origins of amino acids to bacteria, fungi and plants in enchytraeids. Enchytraeids collected from arctic peatlands derived more than 80% of their EAA from bacteria. In a controlled feeding study with the enchytraeid Enchytraeus crypticus, EAA derived almost exclusively from gut bacteria when the worms fed on higher fiber diets, whereas most of the enchytraeids' EAA derived from dietary sources when fed on lower fiber diets. Our gene sequencing results of gut microbiota showed that the worms harbor several taxa in their gut lumen absent from their diets and substrates. Almost all gut taxa are candidates for EAA supplementation because almost all belong to clades capable of biosynthesizing EAA. Our study provides the first evidence of extensive symbiotic supplementation of EAA by microbial gut symbionts, and demonstrate that symbiotic bacteria in the gut lumen appear to function as partners for both symbiotic EAA supplementation as well as for digestion of insoluble plant fibers. Article in Journal/Newspaper Arctic Arctic Alaska Dryad Digital Repository (Duke University) Arctic |
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Open Polar |
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Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
Stable carbon isotopes amino acids |
spellingShingle |
Stable carbon isotopes amino acids Larsen, Thomas Ventura, Marc Maraldo, Kristine Triadó-Margarit, Xavier Casamayor, Emilio O. Wang, Yiming V. Andersen, Nils O'Brien, Diane M. Data from: The dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts |
topic_facet |
Stable carbon isotopes amino acids |
description |
Supplementation of nutrients by symbionts enables consumers to thrive on resources that might otherwise be insufficient to meet nutritional demands. Such nutritional subsidies by intracellular symbionts has been well studied; however, supplementation of de novo synthesized nutrients to hosts by extracellular gut symbionts is poorly documented, especially for generalists with relatively undifferentiated intestinal tracts. Although gut symbionts facilitate degradation of resources that would otherwise remain inaccessible to the host, such digestive actions alone cannot make up for dietary insufficiencies of macronutrients such as essential amino acids (EAA). Documenting whether gut symbionts also function as partners for symbiotic EAA supplementation is important because the question of how some detritivores are able to subsist on nutritionally insufficient diets has remained unresolved. To answer this poorly-understood nutritional aspect of symbiont-host interactions, we studied the enchytraeid worm, a bulk soil feeder that thrives in arctic peatlands. In a combined field and laboratory study, we employed stable isotope fingerprinting of amino acids to identify the biosynthetic origins of amino acids to bacteria, fungi and plants in enchytraeids. Enchytraeids collected from arctic peatlands derived more than 80% of their EAA from bacteria. In a controlled feeding study with the enchytraeid Enchytraeus crypticus, EAA derived almost exclusively from gut bacteria when the worms fed on higher fiber diets, whereas most of the enchytraeids' EAA derived from dietary sources when fed on lower fiber diets. Our gene sequencing results of gut microbiota showed that the worms harbor several taxa in their gut lumen absent from their diets and substrates. Almost all gut taxa are candidates for EAA supplementation because almost all belong to clades capable of biosynthesizing EAA. Our study provides the first evidence of extensive symbiotic supplementation of EAA by microbial gut symbionts, and demonstrate that symbiotic bacteria in the gut lumen appear to function as partners for both symbiotic EAA supplementation as well as for digestion of insoluble plant fibers. |
format |
Article in Journal/Newspaper |
author |
Larsen, Thomas Ventura, Marc Maraldo, Kristine Triadó-Margarit, Xavier Casamayor, Emilio O. Wang, Yiming V. Andersen, Nils O'Brien, Diane M. |
author_facet |
Larsen, Thomas Ventura, Marc Maraldo, Kristine Triadó-Margarit, Xavier Casamayor, Emilio O. Wang, Yiming V. Andersen, Nils O'Brien, Diane M. |
author_sort |
Larsen, Thomas |
title |
Data from: The dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts |
title_short |
Data from: The dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts |
title_full |
Data from: The dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts |
title_fullStr |
Data from: The dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts |
title_full_unstemmed |
Data from: The dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts |
title_sort |
data from: the dominant detritus-feeding invertebrate in arctic peat soils derives its essential amino acids from gut symbionts |
publishDate |
2016 |
url |
http://hdl.handle.net/10255/dryad.118271 https://doi.org/10.5061/dryad.6f798 |
op_coverage |
Alaska Denmark |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic Alaska |
genre_facet |
Arctic Arctic Alaska |
op_relation |
doi:10.5061/dryad.6f798/1 doi:10.1111/1365-2656.12563 PMID:27322934 doi:10.5061/dryad.6f798 Larsen T, Ventura M, Maraldo K, Triadó-Margarit X, Casamayor EO, Wang YV, Andersen N, O'Brien DM (2016) The dominant detritus-feeding invertebrate in Arctic peat soils derives its essential amino acids from gut symbionts. Journal of Animal Ecology 85(5): 1275-1285. 0021-8790 http://hdl.handle.net/10255/dryad.118271 |
op_doi |
https://doi.org/10.5061/dryad.6f798 https://doi.org/10.5061/dryad.6f798/1 https://doi.org/10.1111/1365-2656.12563 |
_version_ |
1766300312542380032 |