Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments
Sea ice is an analog environment for several of astrobiology’s near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to l...
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ftdryad:oai:v1.datadryad.org:10255/dryad.106301 2023-05-15T16:28:14+02:00 Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments Lindensmith, Christian A. Rider, Stephanie Bedrossian, Manuel Wallace, J. Kent Serabyn, Eugene Showalter, Gordon M. Deming, Jody W. Nadeau, Jay L. Greenland Holocene 2016-02-01T21:33:49Z http://hdl.handle.net/10255/dryad.106301 https://doi.org/10.5061/dryad.rc63v unknown doi:10.5061/dryad.rc63v/1 doi:10.5061/dryad.rc63v/2 doi:10.5061/dryad.rc63v/3 doi:10.5061/dryad.rc63v/4 doi:10.5061/dryad.rc63v/5 doi:10.5061/dryad.rc63v/6 doi:10.5061/dryad.rc63v/7 doi:10.5061/dryad.rc63v/8 doi:10.5061/dryad.rc63v/9 doi:10.5061/dryad.rc63v/10 doi:10.5061/dryad.rc63v/11 doi:10.1371/journal.pone.0147700 PMID:26812683 doi:10.5061/dryad.rc63v Lindensmith CA, Rider S, Bedrossian M, Wallace JK, Serabyn E, Showalter GM, Deming JW, Nadeau JL (2016) A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments. PLoS ONE 11(1): e0147700. http://hdl.handle.net/10255/dryad.106301 astrobiology sea ice motility marine microorganisms algae diatoms bacteria Article 2016 ftdryad https://doi.org/10.5061/dryad.rc63v https://doi.org/10.5061/dryad.rc63v/1 https://doi.org/10.5061/dryad.rc63v/2 https://doi.org/10.5061/dryad.rc63v/3 https://doi.org/10.5061/dryad.rc63v/4 https://doi.org/10.5061/dryad.rc63v/5 https://doi.org/1 2020-01-01T15:29:24Z Sea ice is an analog environment for several of astrobiology’s near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to liquid water below in order to detect life. We have developed a submersible digital holographic microscope (DHM) that is capable of resolving individual bacterial cells, and demonstrated its utility for immediately imaging samples taken directly from sea ice at several locations near Nuuk, Greenland. In all samples, the appearance and motility of eukaryotes were conclusive signs of life. The appearance of prokaryotic cells alone was not sufficient to confirm life, but when prokaryotic motility occurred, it was rapid and conclusive. Warming the samples to above-freezing temperatures or supplementing with serine increased the number of motile cells and the speed of motility; supplementing with serine also stimulated chemotaxis. These results show that DHM is a useful technique for detection of active organisms in extreme environments, and that motility may be used as a biosignature in the liquid brines that persist in ice. These findings have important implications for the design of missions to icy environments and suggest ways in which DHM imaging may be integrated with chemical life-detection suites in order to create more conclusive life detection packages. Article in Journal/Newspaper Greenland Nuuk Sea ice Dryad Digital Repository (Duke University) Greenland Nuuk ENVELOPE(-52.150,-52.150,68.717,68.717) |
institution |
Open Polar |
collection |
Dryad Digital Repository (Duke University) |
op_collection_id |
ftdryad |
language |
unknown |
topic |
astrobiology sea ice motility marine microorganisms algae diatoms bacteria |
spellingShingle |
astrobiology sea ice motility marine microorganisms algae diatoms bacteria Lindensmith, Christian A. Rider, Stephanie Bedrossian, Manuel Wallace, J. Kent Serabyn, Eugene Showalter, Gordon M. Deming, Jody W. Nadeau, Jay L. Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
topic_facet |
astrobiology sea ice motility marine microorganisms algae diatoms bacteria |
description |
Sea ice is an analog environment for several of astrobiology’s near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to liquid water below in order to detect life. We have developed a submersible digital holographic microscope (DHM) that is capable of resolving individual bacterial cells, and demonstrated its utility for immediately imaging samples taken directly from sea ice at several locations near Nuuk, Greenland. In all samples, the appearance and motility of eukaryotes were conclusive signs of life. The appearance of prokaryotic cells alone was not sufficient to confirm life, but when prokaryotic motility occurred, it was rapid and conclusive. Warming the samples to above-freezing temperatures or supplementing with serine increased the number of motile cells and the speed of motility; supplementing with serine also stimulated chemotaxis. These results show that DHM is a useful technique for detection of active organisms in extreme environments, and that motility may be used as a biosignature in the liquid brines that persist in ice. These findings have important implications for the design of missions to icy environments and suggest ways in which DHM imaging may be integrated with chemical life-detection suites in order to create more conclusive life detection packages. |
format |
Article in Journal/Newspaper |
author |
Lindensmith, Christian A. Rider, Stephanie Bedrossian, Manuel Wallace, J. Kent Serabyn, Eugene Showalter, Gordon M. Deming, Jody W. Nadeau, Jay L. |
author_facet |
Lindensmith, Christian A. Rider, Stephanie Bedrossian, Manuel Wallace, J. Kent Serabyn, Eugene Showalter, Gordon M. Deming, Jody W. Nadeau, Jay L. |
author_sort |
Lindensmith, Christian A. |
title |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_short |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_full |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_fullStr |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_full_unstemmed |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_sort |
data from: a submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
publishDate |
2016 |
url |
http://hdl.handle.net/10255/dryad.106301 https://doi.org/10.5061/dryad.rc63v |
op_coverage |
Greenland Holocene |
long_lat |
ENVELOPE(-52.150,-52.150,68.717,68.717) |
geographic |
Greenland Nuuk |
geographic_facet |
Greenland Nuuk |
genre |
Greenland Nuuk Sea ice |
genre_facet |
Greenland Nuuk Sea ice |
op_relation |
doi:10.5061/dryad.rc63v/1 doi:10.5061/dryad.rc63v/2 doi:10.5061/dryad.rc63v/3 doi:10.5061/dryad.rc63v/4 doi:10.5061/dryad.rc63v/5 doi:10.5061/dryad.rc63v/6 doi:10.5061/dryad.rc63v/7 doi:10.5061/dryad.rc63v/8 doi:10.5061/dryad.rc63v/9 doi:10.5061/dryad.rc63v/10 doi:10.5061/dryad.rc63v/11 doi:10.1371/journal.pone.0147700 PMID:26812683 doi:10.5061/dryad.rc63v Lindensmith CA, Rider S, Bedrossian M, Wallace JK, Serabyn E, Showalter GM, Deming JW, Nadeau JL (2016) A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments. PLoS ONE 11(1): e0147700. http://hdl.handle.net/10255/dryad.106301 |
op_doi |
https://doi.org/10.5061/dryad.rc63v https://doi.org/10.5061/dryad.rc63v/1 https://doi.org/10.5061/dryad.rc63v/2 https://doi.org/10.5061/dryad.rc63v/3 https://doi.org/10.5061/dryad.rc63v/4 https://doi.org/10.5061/dryad.rc63v/5 https://doi.org/1 |
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1766017858878308352 |