Data from: Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations
Estimating the frequency of hybridization is important to understand its evolutionary consequences and its effects on conservation efforts. In this study, we examined the extent of hybridization in two sister species of ducks that hybridize. We used mitochondrial control region sequences and 3,589 d...
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ftdryad:oai:v1.datadryad.org:10255/dryad.101574 2023-05-15T16:23:10+02:00 Data from: Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations Lavretsky, Philip Peters, Jeffrey Winker, Kevin Bahn, Volker Kulikova, Irina Zhuravlev, Yuri Wilson, Robert Barger, Christopher Gurney, Kirsty McCracken, Kevin 2015-11-19T16:39:38Z http://hdl.handle.net/10255/dryad.101574 https://doi.org/10.5061/dryad.g3g65 unknown doi:10.5061/dryad.g3g65/1 doi:10.5061/dryad.g3g65/2 doi:10.5061/dryad.g3g65/3 doi:10.5061/dryad.g3g65/4 doi:10.1111/mec.13487 PMID:26833858 doi:10.5061/dryad.g3g65 Lavretsky P, Peters J, Winker K, Bahn V, Kulikova I, Zhuravlev Y, Wilson R, Barger C, Gurney K, McCracken K (2015)Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations.Molecular Ecology, 25(3):661–674 http://hdl.handle.net/10255/dryad.101574 ddRADseq Diving Duck Evolution Hybridization Introgression Population Genetics Article 2015 ftdryad https://doi.org/10.5061/dryad.g3g65 https://doi.org/10.5061/dryad.g3g65/1 https://doi.org/10.5061/dryad.g3g65/2 https://doi.org/10.5061/dryad.g3g65/3 https://doi.org/10.5061/dryad.g3g65/4 https://doi.org/10.1111/mec.13487 2020-01-01T15:26:55Z Estimating the frequency of hybridization is important to understand its evolutionary consequences and its effects on conservation efforts. In this study, we examined the extent of hybridization in two sister species of ducks that hybridize. We used mitochondrial control region sequences and 3,589 double-digest restriction-associated DNA sequences (ddRADseq) to identify admixture between wild lesser scaup (Aythya affinis) and greater scaup (A. marila). Among 111 individuals, we found one introgressed mitochondrial DNA haplotype in lesser scaup and four in greater scaup. Likewise, based on the site-frequency spectrum from autosomal DNA, gene flow was asymmetrical, with higher rates from lesser into greater scaup. However, using ddRADseq nuclear DNA all individuals were assigned to their respective species with > 0.95 posterior assignment probability. To examine the power for detecting admixture, we simulated a breeding experiment in which empirical data were used to create F1 hybrids and nine generations (F2-F10) of backcrossing. F1 hybrids and F2, F3, and most F4 backcrosses were clearly distinguishable from pure individuals, but evidence of admixed histories was effectively lost after the fourth generation. Thus, we conclude that low interspecific assignment probabilities (0.011 – 0.043) for two lesser and nineteen greater scaup were consistent with admixed histories beyond the F3 generation. These results indicate that the propensity of these species to hybridize in the wild is low and largely asymmetric. When applied to species-specific cases, our approach offers powerful utility for examining concerns of hybridization in conservation efforts, especially for determining the generational time until admixed histories are effectively lost through backcrossing. Article in Journal/Newspaper greater scaup Dryad Digital Repository (Duke University) |
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Dryad Digital Repository (Duke University) |
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unknown |
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ddRADseq Diving Duck Evolution Hybridization Introgression Population Genetics |
spellingShingle |
ddRADseq Diving Duck Evolution Hybridization Introgression Population Genetics Lavretsky, Philip Peters, Jeffrey Winker, Kevin Bahn, Volker Kulikova, Irina Zhuravlev, Yuri Wilson, Robert Barger, Christopher Gurney, Kirsty McCracken, Kevin Data from: Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations |
topic_facet |
ddRADseq Diving Duck Evolution Hybridization Introgression Population Genetics |
description |
Estimating the frequency of hybridization is important to understand its evolutionary consequences and its effects on conservation efforts. In this study, we examined the extent of hybridization in two sister species of ducks that hybridize. We used mitochondrial control region sequences and 3,589 double-digest restriction-associated DNA sequences (ddRADseq) to identify admixture between wild lesser scaup (Aythya affinis) and greater scaup (A. marila). Among 111 individuals, we found one introgressed mitochondrial DNA haplotype in lesser scaup and four in greater scaup. Likewise, based on the site-frequency spectrum from autosomal DNA, gene flow was asymmetrical, with higher rates from lesser into greater scaup. However, using ddRADseq nuclear DNA all individuals were assigned to their respective species with > 0.95 posterior assignment probability. To examine the power for detecting admixture, we simulated a breeding experiment in which empirical data were used to create F1 hybrids and nine generations (F2-F10) of backcrossing. F1 hybrids and F2, F3, and most F4 backcrosses were clearly distinguishable from pure individuals, but evidence of admixed histories was effectively lost after the fourth generation. Thus, we conclude that low interspecific assignment probabilities (0.011 – 0.043) for two lesser and nineteen greater scaup were consistent with admixed histories beyond the F3 generation. These results indicate that the propensity of these species to hybridize in the wild is low and largely asymmetric. When applied to species-specific cases, our approach offers powerful utility for examining concerns of hybridization in conservation efforts, especially for determining the generational time until admixed histories are effectively lost through backcrossing. |
format |
Article in Journal/Newspaper |
author |
Lavretsky, Philip Peters, Jeffrey Winker, Kevin Bahn, Volker Kulikova, Irina Zhuravlev, Yuri Wilson, Robert Barger, Christopher Gurney, Kirsty McCracken, Kevin |
author_facet |
Lavretsky, Philip Peters, Jeffrey Winker, Kevin Bahn, Volker Kulikova, Irina Zhuravlev, Yuri Wilson, Robert Barger, Christopher Gurney, Kirsty McCracken, Kevin |
author_sort |
Lavretsky, Philip |
title |
Data from: Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations |
title_short |
Data from: Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations |
title_full |
Data from: Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations |
title_fullStr |
Data from: Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations |
title_full_unstemmed |
Data from: Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations |
title_sort |
data from: becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations |
publishDate |
2015 |
url |
http://hdl.handle.net/10255/dryad.101574 https://doi.org/10.5061/dryad.g3g65 |
genre |
greater scaup |
genre_facet |
greater scaup |
op_relation |
doi:10.5061/dryad.g3g65/1 doi:10.5061/dryad.g3g65/2 doi:10.5061/dryad.g3g65/3 doi:10.5061/dryad.g3g65/4 doi:10.1111/mec.13487 PMID:26833858 doi:10.5061/dryad.g3g65 Lavretsky P, Peters J, Winker K, Bahn V, Kulikova I, Zhuravlev Y, Wilson R, Barger C, Gurney K, McCracken K (2015)Becoming pure: identifying generational classes of admixed individuals within lesser and greater scaup populations.Molecular Ecology, 25(3):661–674 http://hdl.handle.net/10255/dryad.101574 |
op_doi |
https://doi.org/10.5061/dryad.g3g65 https://doi.org/10.5061/dryad.g3g65/1 https://doi.org/10.5061/dryad.g3g65/2 https://doi.org/10.5061/dryad.g3g65/3 https://doi.org/10.5061/dryad.g3g65/4 https://doi.org/10.1111/mec.13487 |
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1766011372445892608 |