Tracking Water Contamination Through Identificaton of Adenovirusese by PCR
Advisor: Marie Nguyen of Des Moines University Contamination by fecal matter leads to potential transmission of diseases through water and food. This is a major concern to many water treatment plants as well as the general population. Adenoviruses make up a family of different viruses which may be e...
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ftdrakeuniv:oai:escholarshare.drake.edu:2092/951 2023-05-15T15:41:07+02:00 Tracking Water Contamination Through Identificaton of Adenovirusese by PCR Mpofu, Prudence Hill, Dennis 2009-06-24T13:15:27Z application/pdf http://hdl.handle.net/2092/951 en_US eng DUCURS 2009;10 http://hdl.handle.net/2092/951 Water pollution Feces Adenoviruses Polymerase chain reaction Presentation 2009 ftdrakeuniv 2022-02-18T13:29:20Z Advisor: Marie Nguyen of Des Moines University Contamination by fecal matter leads to potential transmission of diseases through water and food. This is a major concern to many water treatment plants as well as the general population. Adenoviruses make up a family of different viruses which may be excreted in feces and urine. These viruses are non-enveloped and contain a double stranded DNA genome. Adenoviruses are resistant to ultraviolet inactivation and chlorination which makes them more stable than other viruses found in fecal matter. Human Adenovirus, HAdV, has been identified as a good indicator of human viral contaminants because of its prevalence and how easily it is detected in sewage and river water. Diseases associated with adenoviruses include respiratory diseases. Using real-time PCR, a source-tracking method was developed in order to provide information on whether the contamination originates from humans or animals. Human adenovirus and Bovine Adenovirus samples were used to develop the method of identifying the main contaminant of the Des Moines River and Beaver Creek water. Conventional and Real-time Polymerase Chain reaction (PCR) was used to detect which type of adenovirus was present in the samples using different dilutions. This therefore allowed us to determine the source of the contamination. About two-thirds of the water samples tested showed that Human Adenovirus was present, thus indicating human contamination as the main source. Using PCR, it was possible to detect up to 10,000 fold dilution of the human adenovirus genome. Experiments done using Bovine adenovirus showed the detection by PCR to be less sensitive. Drake University, College of Arts and Sciences, Department of Biology, Biochemistry, Cell and Molecular Biology Program Des Moines Water Works Des Moines University Conference Object Beaver Creek Drake University: eScholarShare@Drake |
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Drake University: eScholarShare@Drake |
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English |
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Water pollution Feces Adenoviruses Polymerase chain reaction |
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Water pollution Feces Adenoviruses Polymerase chain reaction Mpofu, Prudence Hill, Dennis Tracking Water Contamination Through Identificaton of Adenovirusese by PCR |
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Water pollution Feces Adenoviruses Polymerase chain reaction |
description |
Advisor: Marie Nguyen of Des Moines University Contamination by fecal matter leads to potential transmission of diseases through water and food. This is a major concern to many water treatment plants as well as the general population. Adenoviruses make up a family of different viruses which may be excreted in feces and urine. These viruses are non-enveloped and contain a double stranded DNA genome. Adenoviruses are resistant to ultraviolet inactivation and chlorination which makes them more stable than other viruses found in fecal matter. Human Adenovirus, HAdV, has been identified as a good indicator of human viral contaminants because of its prevalence and how easily it is detected in sewage and river water. Diseases associated with adenoviruses include respiratory diseases. Using real-time PCR, a source-tracking method was developed in order to provide information on whether the contamination originates from humans or animals. Human adenovirus and Bovine Adenovirus samples were used to develop the method of identifying the main contaminant of the Des Moines River and Beaver Creek water. Conventional and Real-time Polymerase Chain reaction (PCR) was used to detect which type of adenovirus was present in the samples using different dilutions. This therefore allowed us to determine the source of the contamination. About two-thirds of the water samples tested showed that Human Adenovirus was present, thus indicating human contamination as the main source. Using PCR, it was possible to detect up to 10,000 fold dilution of the human adenovirus genome. Experiments done using Bovine adenovirus showed the detection by PCR to be less sensitive. Drake University, College of Arts and Sciences, Department of Biology, Biochemistry, Cell and Molecular Biology Program Des Moines Water Works Des Moines University |
format |
Conference Object |
author |
Mpofu, Prudence Hill, Dennis |
author_facet |
Mpofu, Prudence Hill, Dennis |
author_sort |
Mpofu, Prudence |
title |
Tracking Water Contamination Through Identificaton of Adenovirusese by PCR |
title_short |
Tracking Water Contamination Through Identificaton of Adenovirusese by PCR |
title_full |
Tracking Water Contamination Through Identificaton of Adenovirusese by PCR |
title_fullStr |
Tracking Water Contamination Through Identificaton of Adenovirusese by PCR |
title_full_unstemmed |
Tracking Water Contamination Through Identificaton of Adenovirusese by PCR |
title_sort |
tracking water contamination through identificaton of adenovirusese by pcr |
publishDate |
2009 |
url |
http://hdl.handle.net/2092/951 |
genre |
Beaver Creek |
genre_facet |
Beaver Creek |
op_relation |
DUCURS 2009;10 http://hdl.handle.net/2092/951 |
_version_ |
1766373992380235776 |