Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells

• Published in: International Journal of Nanomedicine
• Main Authors: Ravikiran,Tekupalli, Anand,Santosh, Ansari,Mohammad Azam, Alomary,Mohammad N, AlYahya,Sami, Ramachandregowda,Sowbhagya, Alghamdi,Saad, Sindhghatta Kariyappa,Anupama, Dundaiah,Bhagyalakshmi, Madhugiri Gopinath,Mamatha, Sultana,Sumreen, Punekar,Shital Manohar, Lakshmeesha,Thimappa Ramachandrappa
• Format: Article in Journal/Newspaper
• Language: English
• Published: Dove Press 2021
• Subjects: International Journal of Nanomedicine; Reva; sami
• Online Access: https://www.dovepress.com/fabrication-and-in-vitro-evaluation-of-4-hia-encapsulated-plga-nanopar-peer-reviewed-fulltext-article-IJN

Tekupalli Ravikiran,1 Santosh Anand,2 Mohammad Azam Ansari,3 Mohammad N Alomary,4 Sami AlYahya,4 Sowbhagya Ramachandregowda,1 Saad Alghamdi,5 Anupama Sindhghatta Kariyappa,1 Bhagyalakshmi Dundaiah,1 Mamatha Madhugiri Gopinath,1 Sumreen Sultana,1 Shital Manohar Punekar,1 Thimappa Ramachandrappa Lakshmeesha1 1Department of Biotechnology, Bangalore University, Bangalore, India; 2Department of Biotechnology, School of Applied Sciences, Reva University, Bengaluru, India; 3Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi Arabia; 4National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia; 5Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Thimappa Ramachandrappa LakshmeeshaDepartment of Biotechnology, Bangalore University, Bangalore, 560056, Karnataka, IndiaTel [phone]Email [e-mail] Azam AnsariDepartment of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi ArabiaTel [phone]Email [e-mail]: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decalepis hamiltonii, which has attracted considerable attention in attenuating oxidative stress-related neurodegenerative diseases. However, its efficacy is limited because of its low solubility and bioavailability. Therefore, the present study aimed to encapsulate 4-HIA using biocompatible copolymer polylactide-co-glycolide (PLGA) and evaluate its antioxidant and neuroprotective potential.Methods: The nanoparticles (NPs) were fabricated by solid/oil/water (s/o/w) emulsion technique and characterized using XRD, SEM, HR-TEM, and FTIR spectroscopy. Antioxidant assays such as 1,1 diphenyl-2-picrylhydrazyl (DPPH), superoxide, ...