Stability of thromboxane in blood samples
Helga Helgadóttir,1,2 Ísleifur Ólafsson,2 Karl Andersen,3 Sveinbjörn Gizurarson11Faculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland; 2Department of Clinical Biochemistry, Landspitali University Hospital, Reykjavik, Iceland; 3Faculty of Medicine, University of Iceland, Reyk...
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ftdovepress:oai:dovepress.com/46254 2023-05-15T16:46:45+02:00 Stability of thromboxane in blood samples Helgadóttir,Helga Ólafsson,Ãsleifur Andersen,Karl Gizurarson,Sveinbjörn 2019-06-04 text/html https://www.dovepress.com/stability-of-thromboxane-in-blood-samples-peer-reviewed-fulltext-article-VHRM en eng Dove Press info:eu-repo/semantics/altIdentifier/doi/10.2147/VHRM.S204925 https://www.dovepress.com/stability-of-thromboxane-in-blood-samples-peer-reviewed-fulltext-article-VHRM info:eu-repo/semantics/openAccess Vascular Health and Risk Management Original Research info:eu-repo/semantics/article 2019 ftdovepress https://doi.org/10.2147/VHRM.S204925 2022-12-27T22:36:55Z Helga Helgadóttir,1,2 Ísleifur Ólafsson,2 Karl Andersen,3 Sveinbjörn Gizurarson11Faculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland; 2Department of Clinical Biochemistry, Landspitali University Hospital, Reykjavik, Iceland; 3Faculty of Medicine, University of Iceland, Reykjavik, IcelandIntroduction: Conventional venous blood collection requires a puncture with a needle through the endothelium of a vessel. The endothelial injury causes activation of circulating platelets and the release of thromboxane A2. The aim of the study was to investigate if platelets continue to form thromboxane A2 in the blood tube after sample collection, but such synthesis would give false information about the actual circulating thromboxane A2 value.Methods: Thromboxane B2 is a biologically inactive but stable metabolite of thromboxane A2 and can be measured in blood samples by a standard enzyme immunoassay. Thromboxane B2 measurements reflect thromboxane A2 concentration. Blood samples were collected in 3.2% sodium citrate vials and EDTA vials from ten individuals and centrifuged and frozen at different time points (0, 30, and 120 minutes). Plasma aliquots were transferred to and frozen in 1.8 mL polypropylene tubes and the citrate samples were also transferred to and frozen in propylene tubes containing indomethacin.Results: Concentrations of thromboxane B2 in plasma samples collected in citrate vials and stored in propylene tubes increased very rapidly as the samples were left for longer after sampling and allowed to stand at room temperature. After 120 minutes, the amount of thromboxane B2 was 400% higher than in the reference sample at time zero. In comparison, thromboxane B2 concentration was about 200% higher in the 120-minute samples compared to the reference in samples collected in citrate vials but stored in indomethacin tubes. In samples collected in EDTA vials, a 10% reduction in thromboxane B2 concentration in the 120-minute samples was observed.Conclusion: Storage conditions, type of sampling ... Article in Journal/Newspaper Iceland Dove Medical Press Vascular Health and Risk Management Volume 15 143 147 |
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Vascular Health and Risk Management Helgadóttir,Helga Ólafsson,Ãsleifur Andersen,Karl Gizurarson,Sveinbjörn Stability of thromboxane in blood samples |
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Vascular Health and Risk Management |
description |
Helga Helgadóttir,1,2 Ísleifur Ólafsson,2 Karl Andersen,3 Sveinbjörn Gizurarson11Faculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland; 2Department of Clinical Biochemistry, Landspitali University Hospital, Reykjavik, Iceland; 3Faculty of Medicine, University of Iceland, Reykjavik, IcelandIntroduction: Conventional venous blood collection requires a puncture with a needle through the endothelium of a vessel. The endothelial injury causes activation of circulating platelets and the release of thromboxane A2. The aim of the study was to investigate if platelets continue to form thromboxane A2 in the blood tube after sample collection, but such synthesis would give false information about the actual circulating thromboxane A2 value.Methods: Thromboxane B2 is a biologically inactive but stable metabolite of thromboxane A2 and can be measured in blood samples by a standard enzyme immunoassay. Thromboxane B2 measurements reflect thromboxane A2 concentration. Blood samples were collected in 3.2% sodium citrate vials and EDTA vials from ten individuals and centrifuged and frozen at different time points (0, 30, and 120 minutes). Plasma aliquots were transferred to and frozen in 1.8 mL polypropylene tubes and the citrate samples were also transferred to and frozen in propylene tubes containing indomethacin.Results: Concentrations of thromboxane B2 in plasma samples collected in citrate vials and stored in propylene tubes increased very rapidly as the samples were left for longer after sampling and allowed to stand at room temperature. After 120 minutes, the amount of thromboxane B2 was 400% higher than in the reference sample at time zero. In comparison, thromboxane B2 concentration was about 200% higher in the 120-minute samples compared to the reference in samples collected in citrate vials but stored in indomethacin tubes. In samples collected in EDTA vials, a 10% reduction in thromboxane B2 concentration in the 120-minute samples was observed.Conclusion: Storage conditions, type of sampling ... |
format |
Article in Journal/Newspaper |
author |
Helgadóttir,Helga Ólafsson,Ãsleifur Andersen,Karl Gizurarson,Sveinbjörn |
author_facet |
Helgadóttir,Helga Ólafsson,Ãsleifur Andersen,Karl Gizurarson,Sveinbjörn |
author_sort |
Helgadóttir,Helga |
title |
Stability of thromboxane in blood samples |
title_short |
Stability of thromboxane in blood samples |
title_full |
Stability of thromboxane in blood samples |
title_fullStr |
Stability of thromboxane in blood samples |
title_full_unstemmed |
Stability of thromboxane in blood samples |
title_sort |
stability of thromboxane in blood samples |
publisher |
Dove Press |
publishDate |
2019 |
url |
https://www.dovepress.com/stability-of-thromboxane-in-blood-samples-peer-reviewed-fulltext-article-VHRM |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.2147/VHRM.S204925 https://www.dovepress.com/stability-of-thromboxane-in-blood-samples-peer-reviewed-fulltext-article-VHRM |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.2147/VHRM.S204925 |
container_title |
Vascular Health and Risk Management |
container_volume |
Volume 15 |
container_start_page |
143 |
op_container_end_page |
147 |
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1766036852105543680 |