Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population
Single nucleotide polymorphisms (SNPs) which represent the most widespread source of sequence variation in genomes, are becoming a routine application in several fields such as forensics, ecology and conservation genetics. Their use, requiring short amplifications, may allow a more efficient genotyp...
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Online Access: | https://doi.org/10.2298/ABS1201321F |
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ftdoiserbia:oai:doiserbia:0354-46641201321F 2023-05-15T15:50:23+02:00 Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population Fabbri Elena Caniglia R. Mucci Nadia Thomsen H.P. Krag K. Pertoldi C. Loeschcke V. Randi E. 2012 https://doi.org/10.2298/ABS1201321F unknown Canis lupus fecal samples genotyping errors Pyrosequencing SNaPshot® TaqMan® Assay . 64(1) 321-335 2012 ftdoiserbia https://doi.org/10.2298/ABS1201321F 2018-10-21T10:35:56Z Single nucleotide polymorphisms (SNPs) which represent the most widespread source of sequence variation in genomes, are becoming a routine application in several fields such as forensics, ecology and conservation genetics. Their use, requiring short amplifications, may allow a more efficient genotyping of degraded DNA. We provide the first application of SNP genotyping in an Italian non-invasive genetic monitoring project of the wolf. We compared three different techniques for genotyping SNPs: pyrosequencing, SNaPshot® and TaqMan® Probe Assay in Real-Time PCR. We successively genotyped nine SNPs using the TaqMan Probe Assay in 51 Italian wolves, 57 domestic dogs, 15 wolf x dog hybrids and 313 wolf scats collected in the northern Apennines. The obtained results were used to estimate genetic variability and PCR error rates in SNP genotyping protocols compared to standard microsatellite analysis. We evaluated the cost, laboratory effort and reliability of these different markers and discuss the possible future use of VeraCode, SNPlex and Fluidigm EP1 system in wild population monitoring. Other/Unknown Material Canis lupus doiSerbia (National Library of Serbia/KoBSON) Archives of Biological Sciences 64 1 321 335 |
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doiSerbia (National Library of Serbia/KoBSON) |
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Canis lupus fecal samples genotyping errors Pyrosequencing SNaPshot® TaqMan® Assay |
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Canis lupus fecal samples genotyping errors Pyrosequencing SNaPshot® TaqMan® Assay Fabbri Elena Caniglia R. Mucci Nadia Thomsen H.P. Krag K. Pertoldi C. Loeschcke V. Randi E. Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
topic_facet |
Canis lupus fecal samples genotyping errors Pyrosequencing SNaPshot® TaqMan® Assay |
description |
Single nucleotide polymorphisms (SNPs) which represent the most widespread source of sequence variation in genomes, are becoming a routine application in several fields such as forensics, ecology and conservation genetics. Their use, requiring short amplifications, may allow a more efficient genotyping of degraded DNA. We provide the first application of SNP genotyping in an Italian non-invasive genetic monitoring project of the wolf. We compared three different techniques for genotyping SNPs: pyrosequencing, SNaPshot® and TaqMan® Probe Assay in Real-Time PCR. We successively genotyped nine SNPs using the TaqMan Probe Assay in 51 Italian wolves, 57 domestic dogs, 15 wolf x dog hybrids and 313 wolf scats collected in the northern Apennines. The obtained results were used to estimate genetic variability and PCR error rates in SNP genotyping protocols compared to standard microsatellite analysis. We evaluated the cost, laboratory effort and reliability of these different markers and discuss the possible future use of VeraCode, SNPlex and Fluidigm EP1 system in wild population monitoring. |
format |
Other/Unknown Material |
author |
Fabbri Elena Caniglia R. Mucci Nadia Thomsen H.P. Krag K. Pertoldi C. Loeschcke V. Randi E. |
author_facet |
Fabbri Elena Caniglia R. Mucci Nadia Thomsen H.P. Krag K. Pertoldi C. Loeschcke V. Randi E. |
author_sort |
Fabbri Elena |
title |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_short |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_full |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_fullStr |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_full_unstemmed |
Comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
title_sort |
comparison of single nucleotide polymorphisms and microsatellites in non-invasive genetic monitoring of a wolf population |
publishDate |
2012 |
url |
https://doi.org/10.2298/ABS1201321F |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_doi |
https://doi.org/10.2298/ABS1201321F |
container_title |
Archives of Biological Sciences |
container_volume |
64 |
container_issue |
1 |
container_start_page |
321 |
op_container_end_page |
335 |
_version_ |
1766385331405324288 |