Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions

Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to...

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Bibliographic Details
Published in:International Journal of Molecular Sciences
Main Authors: Udo Conrad, Hoang Trong Phan
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2011
Subjects:
transgenic plants
avian influenza
H5N1
nanobody
ELPylation
Inverse Transition Cycling
Biology (General)
QH301-705.5
Chemistry
QD1-999
Avian flu
Online Access:https://doi.org/10.3390/ijms12052808
https://doaj.org/article/ffbcdf3a54fd4fdca08ffbb468c830f0
id ftdoajarticles:oai:doaj.org/article:ffbcdf3a54fd4fdca08ffbb468c830f0
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spelling ftdoajarticles:oai:doaj.org/article:ffbcdf3a54fd4fdca08ffbb468c830f0 2023-05-15T15:34:19+02:00 Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions Udo Conrad Hoang Trong Phan 2011-04-01T00:00:00Z https://doi.org/10.3390/ijms12052808 https://doaj.org/article/ffbcdf3a54fd4fdca08ffbb468c830f0 EN eng MDPI AG http://www.mdpi.com/1422-0067/12/5/2808/ https://doaj.org/toc/1422-0067 doi:10.3390/ijms12052808 1422-0067 https://doaj.org/article/ffbcdf3a54fd4fdca08ffbb468c830f0 International Journal of Molecular Sciences, Vol 12, Iss 5, Pp 2808-2821 (2011) transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling Biology (General) QH301-705.5 Chemistry QD1-999 article 2011 ftdoajarticles https://doi.org/10.3390/ijms12052808 2022-12-31T00:50:56Z Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated. Article in Journal/Newspaper Avian flu Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 12 5 2808 2821
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic transgenic plants
avian influenza
H5N1
nanobody
ELPylation
Inverse Transition Cycling
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle transgenic plants
avian influenza
H5N1
nanobody
ELPylation
Inverse Transition Cycling
Biology (General)
QH301-705.5
Chemistry
QD1-999
Udo Conrad
Hoang Trong Phan
Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions
topic_facet transgenic plants
avian influenza
H5N1
nanobody
ELPylation
Inverse Transition Cycling
Biology (General)
QH301-705.5
Chemistry
QD1-999
description Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated.
format Article in Journal/Newspaper
author Udo Conrad
Hoang Trong Phan
author_facet Udo Conrad
Hoang Trong Phan
author_sort Udo Conrad
title Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions
title_short Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions
title_full Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions
title_fullStr Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions
title_full_unstemmed Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions
title_sort membrane-based inverse transition cycling: an improved means for purifying plant-derived recombinant protein-elastin-like polypeptide fusions
publisher MDPI AG
publishDate 2011
url https://doi.org/10.3390/ijms12052808
https://doaj.org/article/ffbcdf3a54fd4fdca08ffbb468c830f0
genre Avian flu
genre_facet Avian flu
op_source International Journal of Molecular Sciences, Vol 12, Iss 5, Pp 2808-2821 (2011)
op_relation http://www.mdpi.com/1422-0067/12/5/2808/
https://doaj.org/toc/1422-0067
doi:10.3390/ijms12052808
1422-0067
https://doaj.org/article/ffbcdf3a54fd4fdca08ffbb468c830f0
op_doi https://doi.org/10.3390/ijms12052808
container_title International Journal of Molecular Sciences
container_volume 12
container_issue 5
container_start_page 2808
op_container_end_page 2821
_version_ 1766364755470057472

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