Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna
The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly unde...
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ftdoajarticles:oai:doaj.org/article:ffa9858e7e3d45269449d32fb1570e78 2023-05-15T14:58:02+02:00 Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna Cindy Given Elina Häikiö Manoj Kumar Riitta Nissinen 2020-05-01T00:00:00Z https://doi.org/10.3389/fpls.2020.00561 https://doaj.org/article/ffa9858e7e3d45269449d32fb1570e78 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fpls.2020.00561/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2020.00561 https://doaj.org/article/ffa9858e7e3d45269449d32fb1570e78 Frontiers in Plant Science, Vol 11 (2020) endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria Plant culture SB1-1110 article 2020 ftdoajarticles https://doi.org/10.3389/fpls.2020.00561 2022-12-31T15:19:13Z The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this ... Article in Journal/Newspaper Arctic Oxyria digyna Directory of Open Access Journals: DOAJ Articles Arctic Frontiers in Plant Science 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria Plant culture SB1-1110 |
spellingShingle |
endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria Plant culture SB1-1110 Cindy Given Elina Häikiö Manoj Kumar Riitta Nissinen Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
topic_facet |
endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria Plant culture SB1-1110 |
description |
The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this ... |
format |
Article in Journal/Newspaper |
author |
Cindy Given Elina Häikiö Manoj Kumar Riitta Nissinen |
author_facet |
Cindy Given Elina Häikiö Manoj Kumar Riitta Nissinen |
author_sort |
Cindy Given |
title |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_short |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_full |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_fullStr |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_full_unstemmed |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_sort |
tissue-specific dynamics in the endophytic bacterial communities in arctic pioneer plant oxyria digyna |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fpls.2020.00561 https://doaj.org/article/ffa9858e7e3d45269449d32fb1570e78 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Oxyria digyna |
genre_facet |
Arctic Oxyria digyna |
op_source |
Frontiers in Plant Science, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fpls.2020.00561/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2020.00561 https://doaj.org/article/ffa9858e7e3d45269449d32fb1570e78 |
op_doi |
https://doi.org/10.3389/fpls.2020.00561 |
container_title |
Frontiers in Plant Science |
container_volume |
11 |
_version_ |
1766330118195642368 |