Suppression of virus specific immune responses by IL-10 in acute dengue infection.
Elevated IL-10 has been shown to be associated with severe dengue infection (DI). We proceeded to investigate the role of IL-10 in the pathogenesis of acute DI.Ex vivo and cultured IFNγ ELISpot assays for dengue virus (DENV) NS3 protein and non dengue viral proteins were carried out in 26 patients w...
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ftdoajarticles:oai:doaj.org/article:ff882d3edc04428abaee42571ce86717 2023-05-15T15:10:28+02:00 Suppression of virus specific immune responses by IL-10 in acute dengue infection. Gathsaurie Neelika Malavige Chandima Jeewandara K M Luckmaal Alles Maryam Salimi Laksiri Gomes Achala Kamaladasa S D Jayaratne Graham Stuart Ogg 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002409 https://doaj.org/article/ff882d3edc04428abaee42571ce86717 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3764236?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002409 https://doaj.org/article/ff882d3edc04428abaee42571ce86717 PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2409 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002409 2022-12-31T09:50:09Z Elevated IL-10 has been shown to be associated with severe dengue infection (DI). We proceeded to investigate the role of IL-10 in the pathogenesis of acute DI.Ex vivo and cultured IFNγ ELISpot assays for dengue virus (DENV) NS3 protein and non dengue viral proteins were carried out in 26 patients with acute DI (16 with dengue haemorrhagic fever) and 12 healthy dengue seropositive individuals from Sri Lanka. DENV serotype specific (SS) responses were determined by using a panel of SS peptides.Serum IL-10 level were significantly higher (p = 0.02) in those who did not have in vitro responses to DENV-SS peptides (mean 144.2 pg/ml) when compared to those who responded (mean 75.7 pg/ml). DENV-NS3 specific ex vivo IFNγ ELISpot responses were also significantly lower (p = 0.0001) in those who did not respond to DENV-SS peptides (mean 42 SFU/million PBMCs) when compared to those who responded to DENV-SS peptides (mean 1024 SFU/million PBMCs). Serum IL-10 levels correlated significantly (p = 0.03) and inversely (Spearmans R = -0.45) with ex vivo DENV-NS3 specific responses but not with ex vivo non DENV specific responses (Spearmans R = -014, p = 0.52). Blockage of IL-10 in vitro significantly increased (p = 0.04) the ex vivo IFNγ ELISpot DENV-NS3 specific responses but had no effect on responses to non DENV proteins.IL-10 appears to contribute to the pathogenesis of acute dengue infections by inhibiting DENV-specific T cell responses, which can be restored by blocking IL-10. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 9 e2409 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gathsaurie Neelika Malavige Chandima Jeewandara K M Luckmaal Alles Maryam Salimi Laksiri Gomes Achala Kamaladasa S D Jayaratne Graham Stuart Ogg Suppression of virus specific immune responses by IL-10 in acute dengue infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Elevated IL-10 has been shown to be associated with severe dengue infection (DI). We proceeded to investigate the role of IL-10 in the pathogenesis of acute DI.Ex vivo and cultured IFNγ ELISpot assays for dengue virus (DENV) NS3 protein and non dengue viral proteins were carried out in 26 patients with acute DI (16 with dengue haemorrhagic fever) and 12 healthy dengue seropositive individuals from Sri Lanka. DENV serotype specific (SS) responses were determined by using a panel of SS peptides.Serum IL-10 level were significantly higher (p = 0.02) in those who did not have in vitro responses to DENV-SS peptides (mean 144.2 pg/ml) when compared to those who responded (mean 75.7 pg/ml). DENV-NS3 specific ex vivo IFNγ ELISpot responses were also significantly lower (p = 0.0001) in those who did not respond to DENV-SS peptides (mean 42 SFU/million PBMCs) when compared to those who responded to DENV-SS peptides (mean 1024 SFU/million PBMCs). Serum IL-10 levels correlated significantly (p = 0.03) and inversely (Spearmans R = -0.45) with ex vivo DENV-NS3 specific responses but not with ex vivo non DENV specific responses (Spearmans R = -014, p = 0.52). Blockage of IL-10 in vitro significantly increased (p = 0.04) the ex vivo IFNγ ELISpot DENV-NS3 specific responses but had no effect on responses to non DENV proteins.IL-10 appears to contribute to the pathogenesis of acute dengue infections by inhibiting DENV-specific T cell responses, which can be restored by blocking IL-10. |
format |
Article in Journal/Newspaper |
author |
Gathsaurie Neelika Malavige Chandima Jeewandara K M Luckmaal Alles Maryam Salimi Laksiri Gomes Achala Kamaladasa S D Jayaratne Graham Stuart Ogg |
author_facet |
Gathsaurie Neelika Malavige Chandima Jeewandara K M Luckmaal Alles Maryam Salimi Laksiri Gomes Achala Kamaladasa S D Jayaratne Graham Stuart Ogg |
author_sort |
Gathsaurie Neelika Malavige |
title |
Suppression of virus specific immune responses by IL-10 in acute dengue infection. |
title_short |
Suppression of virus specific immune responses by IL-10 in acute dengue infection. |
title_full |
Suppression of virus specific immune responses by IL-10 in acute dengue infection. |
title_fullStr |
Suppression of virus specific immune responses by IL-10 in acute dengue infection. |
title_full_unstemmed |
Suppression of virus specific immune responses by IL-10 in acute dengue infection. |
title_sort |
suppression of virus specific immune responses by il-10 in acute dengue infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002409 https://doaj.org/article/ff882d3edc04428abaee42571ce86717 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2409 (2013) |
op_relation |
http://europepmc.org/articles/PMC3764236?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002409 https://doaj.org/article/ff882d3edc04428abaee42571ce86717 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002409 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
9 |
container_start_page |
e2409 |
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1766341496140726272 |