Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities
Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activitie...
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ftdoajarticles:oai:doaj.org/article:ff265506c71441fcad7a700bc61bbfbf 2023-05-15T13:34:09+02:00 Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities Lavín Paris Atala Cristian Gallardo-Cerda Jorge Gonzalez-Aravena Marcelo De La Iglesia Rodrigo Oses Rómulo Torres-Díaz Cristian Trefault Nicole Molina-Montenegro Marco A. Laughinghouse IV H. Dail 2016-09-01T00:00:00Z https://doi.org/10.1515/popore-2016-0021 https://doaj.org/article/ff265506c71441fcad7a700bc61bbfbf EN eng Polish Academy of Sciences http://www.degruyter.com/view/j/popore.2016.37.issue-3/popore-2016-0021/popore-2016-0021.xml?format=INT https://doaj.org/toc/2081-8262 2081-8262 doi:10.1515/popore-2016-0021 https://doaj.org/article/ff265506c71441fcad7a700bc61bbfbf Polish Polar Research, Vol 37, Iss 3, Pp 403-419 (2016) Antarctic King George Island Flavobacterium agarase alginate-lyase Geology QE1-996.5 article 2016 ftdoajarticles https://doi.org/10.1515/popore-2016-0021 2022-12-31T04:26:28Z Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17ºC) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Polar Research Directory of Open Access Journals: DOAJ Articles Antarctic King George Island Polish Polar Research 37 3 403 419 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic King George Island Flavobacterium agarase alginate-lyase Geology QE1-996.5 |
spellingShingle |
Antarctic King George Island Flavobacterium agarase alginate-lyase Geology QE1-996.5 Lavín Paris Atala Cristian Gallardo-Cerda Jorge Gonzalez-Aravena Marcelo De La Iglesia Rodrigo Oses Rómulo Torres-Díaz Cristian Trefault Nicole Molina-Montenegro Marco A. Laughinghouse IV H. Dail Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
topic_facet |
Antarctic King George Island Flavobacterium agarase alginate-lyase Geology QE1-996.5 |
description |
Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17ºC) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures. |
format |
Article in Journal/Newspaper |
author |
Lavín Paris Atala Cristian Gallardo-Cerda Jorge Gonzalez-Aravena Marcelo De La Iglesia Rodrigo Oses Rómulo Torres-Díaz Cristian Trefault Nicole Molina-Montenegro Marco A. Laughinghouse IV H. Dail |
author_facet |
Lavín Paris Atala Cristian Gallardo-Cerda Jorge Gonzalez-Aravena Marcelo De La Iglesia Rodrigo Oses Rómulo Torres-Díaz Cristian Trefault Nicole Molina-Montenegro Marco A. Laughinghouse IV H. Dail |
author_sort |
Lavín Paris |
title |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_short |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_full |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_fullStr |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_full_unstemmed |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_sort |
isolation and characterization of an antarctic flavobacterium strain with agarase and alginate lyase activities |
publisher |
Polish Academy of Sciences |
publishDate |
2016 |
url |
https://doi.org/10.1515/popore-2016-0021 https://doaj.org/article/ff265506c71441fcad7a700bc61bbfbf |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island Polar Research |
genre_facet |
Antarc* Antarctic Antarctica King George Island Polar Research |
op_source |
Polish Polar Research, Vol 37, Iss 3, Pp 403-419 (2016) |
op_relation |
http://www.degruyter.com/view/j/popore.2016.37.issue-3/popore-2016-0021/popore-2016-0021.xml?format=INT https://doaj.org/toc/2081-8262 2081-8262 doi:10.1515/popore-2016-0021 https://doaj.org/article/ff265506c71441fcad7a700bc61bbfbf |
op_doi |
https://doi.org/10.1515/popore-2016-0021 |
container_title |
Polish Polar Research |
container_volume |
37 |
container_issue |
3 |
container_start_page |
403 |
op_container_end_page |
419 |
_version_ |
1766049284206100480 |