HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION
SUMMARY Inflammation due to Shigella flexneri can cause damage to the colonic mucosa and cell death by necrosis and apoptosis. This bacteria can reach the bloodstream in this way, and the liver through portal veins. Hypoxia is a condition present in many human diseases, and it may induce bacterial t...
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ftdoajarticles:oai:doaj.org/article:ff1cbd0a89f84f89b6441277d2150391 2024-09-09T19:27:56+00:00 HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION Camila Barbara Cantalupo Lima Sania Alves dos Santos Dahir Ramos de Andrade Junior 2013-09-01T00:00:00Z https://doi.org/10.1590/S0036-46652013000500008 https://doaj.org/article/ff1cbd0a89f84f89b6441277d2150391 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000500341&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652013000500008 https://doaj.org/article/ff1cbd0a89f84f89b6441277d2150391 Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 5, Pp 341-346 (2013) Shigella flexneri Hepatocytes Caco-2 cells Hypoxia Apoptosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2013 ftdoajarticles https://doi.org/10.1590/S0036-46652013000500008 2024-08-05T17:49:31Z SUMMARY Inflammation due to Shigella flexneri can cause damage to the colonic mucosa and cell death by necrosis and apoptosis. This bacteria can reach the bloodstream in this way, and the liver through portal veins. Hypoxia is a condition present in many human diseases, and it may induce bacterial translocation from intestinal lumen. We studied the ability of S. flexneri to invade rat hepatocytes and Caco-2 cells both in normoxic and hypoxic microenvironments, as well as morphological and physiological alterations in these cells after infection under hypoxia. We used the primary culture of rat hepatocytes as a model of study. We analyzed the following parameters in normoxic and hypoxic conditions: morphology, cell viability, bacterial recovery and lactate dehydrogenase (LDH) released. The results showed that there were fewer bacteria within the Caco-2 cells than in hepatocytes in normoxic and hypoxic conditions. We observed that the higher the multiplicity of infection (MOI) the greater the bacterial recovery in hepatocytes. The hypoxic condition decreased the bacterial recovery in hepatocytes. The cytotoxicity evaluated by LDH released by cells was significantly higher in cells submitted to hypoxia than normoxia. Caco-2 cells in normoxia released 63% more LDH than hepatocytes. LDH increased 164% when hepatocytes were submitted to hypoxia and just 21% when Caco-2 cells were in the same condition. The apoptosis evaluated by Tunel was significantly higher in cells submitted to hypoxia than normoxia. When comparing hypoxic cells, we obtained more apoptotic hepatocytes than apoptotic Caco-2 cells. Concluding our results contribute to a better knowledge of interactions between studied cells and Shigella flexneri. These data may be useful in the future to define strategies to combat this virulent pathogen. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 55 5 341 346 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Shigella flexneri Hepatocytes Caco-2 cells Hypoxia Apoptosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Shigella flexneri Hepatocytes Caco-2 cells Hypoxia Apoptosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Camila Barbara Cantalupo Lima Sania Alves dos Santos Dahir Ramos de Andrade Junior HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION |
topic_facet |
Shigella flexneri Hepatocytes Caco-2 cells Hypoxia Apoptosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
SUMMARY Inflammation due to Shigella flexneri can cause damage to the colonic mucosa and cell death by necrosis and apoptosis. This bacteria can reach the bloodstream in this way, and the liver through portal veins. Hypoxia is a condition present in many human diseases, and it may induce bacterial translocation from intestinal lumen. We studied the ability of S. flexneri to invade rat hepatocytes and Caco-2 cells both in normoxic and hypoxic microenvironments, as well as morphological and physiological alterations in these cells after infection under hypoxia. We used the primary culture of rat hepatocytes as a model of study. We analyzed the following parameters in normoxic and hypoxic conditions: morphology, cell viability, bacterial recovery and lactate dehydrogenase (LDH) released. The results showed that there were fewer bacteria within the Caco-2 cells than in hepatocytes in normoxic and hypoxic conditions. We observed that the higher the multiplicity of infection (MOI) the greater the bacterial recovery in hepatocytes. The hypoxic condition decreased the bacterial recovery in hepatocytes. The cytotoxicity evaluated by LDH released by cells was significantly higher in cells submitted to hypoxia than normoxia. Caco-2 cells in normoxia released 63% more LDH than hepatocytes. LDH increased 164% when hepatocytes were submitted to hypoxia and just 21% when Caco-2 cells were in the same condition. The apoptosis evaluated by Tunel was significantly higher in cells submitted to hypoxia than normoxia. When comparing hypoxic cells, we obtained more apoptotic hepatocytes than apoptotic Caco-2 cells. Concluding our results contribute to a better knowledge of interactions between studied cells and Shigella flexneri. These data may be useful in the future to define strategies to combat this virulent pathogen. |
format |
Article in Journal/Newspaper |
author |
Camila Barbara Cantalupo Lima Sania Alves dos Santos Dahir Ramos de Andrade Junior |
author_facet |
Camila Barbara Cantalupo Lima Sania Alves dos Santos Dahir Ramos de Andrade Junior |
author_sort |
Camila Barbara Cantalupo Lima |
title |
HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION |
title_short |
HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION |
title_full |
HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION |
title_fullStr |
HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION |
title_full_unstemmed |
HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION |
title_sort |
hypoxic stress, hepatocytes and caco-2 viability and susceptibility to shigella flexneri invasion |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2013 |
url |
https://doi.org/10.1590/S0036-46652013000500008 https://doaj.org/article/ff1cbd0a89f84f89b6441277d2150391 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 5, Pp 341-346 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000500341&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652013000500008 https://doaj.org/article/ff1cbd0a89f84f89b6441277d2150391 |
op_doi |
https://doi.org/10.1590/S0036-46652013000500008 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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55 |
container_issue |
5 |
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341 |
op_container_end_page |
346 |
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