Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp.
Leptospirosis is a worldwide spread zoonotic and neglected infectious disease of human and veterinary concern that is caused by pathogenic Leptospira species. In severe infections, hemostatic impairments such as coagulation/fibrinolysis dysfunction are frequently observed. These complications often...
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ftdoajarticles:oai:doaj.org/article:fef4cbd6c2504e62b6d7f4988b062962 2023-05-15T15:09:17+02:00 Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. Mônica L Vieira Clément Naudin Matthias Mörgelin Eliete C Romero Ana Lucia T O Nascimento Heiko Herwald 2016-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004713 https://doaj.org/article/fef4cbd6c2504e62b6d7f4988b062962 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4864083?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004713 https://doaj.org/article/fef4cbd6c2504e62b6d7f4988b062962 PLoS Neglected Tropical Diseases, Vol 10, Iss 5, p e0004713 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004713 2022-12-31T02:16:08Z Leptospirosis is a worldwide spread zoonotic and neglected infectious disease of human and veterinary concern that is caused by pathogenic Leptospira species. In severe infections, hemostatic impairments such as coagulation/fibrinolysis dysfunction are frequently observed. These complications often occur when the host response is controlled and/or modulated by the bacterial pathogen. In the present investigation, we aimed to analyze the modulation of the hemostatic and inflammatory host responses by the bacterial pathogen Leptospira. The effects of leptospires and their secreted products on stimulation of human intrinsic and extrinsic pathways of coagulation were investigated by means of altered clotting times, assembly and activation of contact system and induction of tissue factor. We show that both extrinsic and intrinsic coagulation cascades are modulated in response to Leptospira or leptospiral secreted proteins. We further find that the pro-inflammatory mediator bradykinin is released following contact activation at the bacterial surface and that pro-coagulant microvesicles are shed from monocytes in response to infection. Also, we show that human leptospirosis patients present higher levels of circulating pro-coagulant microvesicles than healthy individuals. Here we show that both pathways of the coagulation system are modulated by leptospires, possibly leading to altered hemostatic and inflammatory responses during the disease. Our results contribute to the understanding of the leptospirosis pathophysiological mechanisms and may open new routes for the discovery of novel treatments for the severe manifestations of the disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 5 e0004713 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mônica L Vieira Clément Naudin Matthias Mörgelin Eliete C Romero Ana Lucia T O Nascimento Heiko Herwald Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leptospirosis is a worldwide spread zoonotic and neglected infectious disease of human and veterinary concern that is caused by pathogenic Leptospira species. In severe infections, hemostatic impairments such as coagulation/fibrinolysis dysfunction are frequently observed. These complications often occur when the host response is controlled and/or modulated by the bacterial pathogen. In the present investigation, we aimed to analyze the modulation of the hemostatic and inflammatory host responses by the bacterial pathogen Leptospira. The effects of leptospires and their secreted products on stimulation of human intrinsic and extrinsic pathways of coagulation were investigated by means of altered clotting times, assembly and activation of contact system and induction of tissue factor. We show that both extrinsic and intrinsic coagulation cascades are modulated in response to Leptospira or leptospiral secreted proteins. We further find that the pro-inflammatory mediator bradykinin is released following contact activation at the bacterial surface and that pro-coagulant microvesicles are shed from monocytes in response to infection. Also, we show that human leptospirosis patients present higher levels of circulating pro-coagulant microvesicles than healthy individuals. Here we show that both pathways of the coagulation system are modulated by leptospires, possibly leading to altered hemostatic and inflammatory responses during the disease. Our results contribute to the understanding of the leptospirosis pathophysiological mechanisms and may open new routes for the discovery of novel treatments for the severe manifestations of the disease. |
format |
Article in Journal/Newspaper |
author |
Mônica L Vieira Clément Naudin Matthias Mörgelin Eliete C Romero Ana Lucia T O Nascimento Heiko Herwald |
author_facet |
Mônica L Vieira Clément Naudin Matthias Mörgelin Eliete C Romero Ana Lucia T O Nascimento Heiko Herwald |
author_sort |
Mônica L Vieira |
title |
Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. |
title_short |
Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. |
title_full |
Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. |
title_fullStr |
Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. |
title_full_unstemmed |
Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. |
title_sort |
modulation of hemostatic and inflammatory responses by leptospira spp. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004713 https://doaj.org/article/fef4cbd6c2504e62b6d7f4988b062962 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 5, p e0004713 (2016) |
op_relation |
http://europepmc.org/articles/PMC4864083?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004713 https://doaj.org/article/fef4cbd6c2504e62b6d7f4988b062962 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004713 |
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PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
5 |
container_start_page |
e0004713 |
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