Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand.
Burkholderia pseudomallei is a soil-dwelling bacterium endemic to Southeast Asia and northern Australia that causes the disease, melioidosis. Although the global genomic diversity of clinical B. pseudomallei isolates has been investigated, there is limited understanding of its genomic diversity acro...
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ftdoajarticles:oai:doaj.org/article:fe70c47d2d794018b4a4d9913862c939 2023-05-15T15:15:35+02:00 Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand. Chandler Roe Adam J Vazquez Paul D Phillips Chris J Allender Richard A Bowen Roxanne D Nottingham Adina Doyle Gumphol Wongsuwan Vanaporn Wuthiekanun Direk Limmathurotsakul Sharon Peacock Paul Keim Apichai Tuanyok David M Wagner Jason W Sahl 2022-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010172 https://doaj.org/article/fe70c47d2d794018b4a4d9913862c939 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010172 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010172 https://doaj.org/article/fe70c47d2d794018b4a4d9913862c939 PLoS Neglected Tropical Diseases, Vol 16, Iss 2, p e0010172 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010172 2022-12-31T01:38:46Z Burkholderia pseudomallei is a soil-dwelling bacterium endemic to Southeast Asia and northern Australia that causes the disease, melioidosis. Although the global genomic diversity of clinical B. pseudomallei isolates has been investigated, there is limited understanding of its genomic diversity across small geographic scales, especially in soil. In this study, we obtained 288 B. pseudomallei isolates from a single soil sample (~100g; intensive site 2, INT2) collected at a depth of 30cm from a site in Ubon Ratchathani Province, Thailand. We sequenced the genomes of 169 of these isolates that represent 7 distinct sequence types (STs), including a new ST (ST1820), based on multi-locus sequence typing (MLST) analysis. A core genome SNP phylogeny demonstrated that all identified STs share a recent common ancestor that diverged an estimated 796-1260 years ago. A pan-genomics analysis demonstrated recombination between clades and intra-MLST phylogenetic and gene differences. To identify potential differential virulence between STs, groups of BALB/c mice (5 mice/isolate) were challenged via subcutaneous injection (500 CFUs) with 30 INT2 isolates representing 5 different STs; over the 21-day experiment, eight isolates killed all mice, 2 isolates killed an intermediate number of mice (1-2), and 20 isolates killed no mice. Although the virulence results were largely stratified by ST, one virulent isolate and six attenuated isolates were from the same ST (ST1005), suggesting that variably conserved genomic regions may contribute to virulence. Genomes from the animal-challenged isolates were subjected to a bacterial genome-wide association study to identify genomic regions associated with differential virulence. One associated region is a unique variant of Hcp1, a component of the type VI secretion system, which may result in attenuation. The results of this study have implications for comprehensive sampling strategies, environmental exposure risk assessment, and understanding recombination and differential virulence in B. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 2 e0010172 |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Chandler Roe Adam J Vazquez Paul D Phillips Chris J Allender Richard A Bowen Roxanne D Nottingham Adina Doyle Gumphol Wongsuwan Vanaporn Wuthiekanun Direk Limmathurotsakul Sharon Peacock Paul Keim Apichai Tuanyok David M Wagner Jason W Sahl Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Burkholderia pseudomallei is a soil-dwelling bacterium endemic to Southeast Asia and northern Australia that causes the disease, melioidosis. Although the global genomic diversity of clinical B. pseudomallei isolates has been investigated, there is limited understanding of its genomic diversity across small geographic scales, especially in soil. In this study, we obtained 288 B. pseudomallei isolates from a single soil sample (~100g; intensive site 2, INT2) collected at a depth of 30cm from a site in Ubon Ratchathani Province, Thailand. We sequenced the genomes of 169 of these isolates that represent 7 distinct sequence types (STs), including a new ST (ST1820), based on multi-locus sequence typing (MLST) analysis. A core genome SNP phylogeny demonstrated that all identified STs share a recent common ancestor that diverged an estimated 796-1260 years ago. A pan-genomics analysis demonstrated recombination between clades and intra-MLST phylogenetic and gene differences. To identify potential differential virulence between STs, groups of BALB/c mice (5 mice/isolate) were challenged via subcutaneous injection (500 CFUs) with 30 INT2 isolates representing 5 different STs; over the 21-day experiment, eight isolates killed all mice, 2 isolates killed an intermediate number of mice (1-2), and 20 isolates killed no mice. Although the virulence results were largely stratified by ST, one virulent isolate and six attenuated isolates were from the same ST (ST1005), suggesting that variably conserved genomic regions may contribute to virulence. Genomes from the animal-challenged isolates were subjected to a bacterial genome-wide association study to identify genomic regions associated with differential virulence. One associated region is a unique variant of Hcp1, a component of the type VI secretion system, which may result in attenuation. The results of this study have implications for comprehensive sampling strategies, environmental exposure risk assessment, and understanding recombination and differential virulence in B. ... |
format |
Article in Journal/Newspaper |
author |
Chandler Roe Adam J Vazquez Paul D Phillips Chris J Allender Richard A Bowen Roxanne D Nottingham Adina Doyle Gumphol Wongsuwan Vanaporn Wuthiekanun Direk Limmathurotsakul Sharon Peacock Paul Keim Apichai Tuanyok David M Wagner Jason W Sahl |
author_facet |
Chandler Roe Adam J Vazquez Paul D Phillips Chris J Allender Richard A Bowen Roxanne D Nottingham Adina Doyle Gumphol Wongsuwan Vanaporn Wuthiekanun Direk Limmathurotsakul Sharon Peacock Paul Keim Apichai Tuanyok David M Wagner Jason W Sahl |
author_sort |
Chandler Roe |
title |
Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand. |
title_short |
Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand. |
title_full |
Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand. |
title_fullStr |
Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand. |
title_full_unstemmed |
Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand. |
title_sort |
multiple phylogenetically-diverse, differentially-virulent burkholderia pseudomallei isolated from a single soil sample collected in thailand. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010172 https://doaj.org/article/fe70c47d2d794018b4a4d9913862c939 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 2, p e0010172 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010172 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010172 https://doaj.org/article/fe70c47d2d794018b4a4d9913862c939 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010172 |
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PLOS Neglected Tropical Diseases |
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16 |
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2 |
container_start_page |
e0010172 |
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