Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh
The sea sandwort— Honckenya peploides (L.) Ehrh. is—a rare halophilous plant growing on dunes and is an endangered species on the Polish coast. It contributes to the stabilization of volatile sandy substrate, facilitating the colonization of other species. The present study determined the reaction o...
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ftdoajarticles:oai:doaj.org/article:fe2c27581c954525863022458db57f9f 2023-05-15T16:34:47+02:00 Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh Danuta Kulpa Mariola Wrobel Martyna Bednarek 2020-11-01T00:00:00Z https://doi.org/10.3390/plants9111526 https://doaj.org/article/fe2c27581c954525863022458db57f9f EN eng MDPI AG https://www.mdpi.com/2223-7747/9/11/1526 https://doaj.org/toc/2223-7747 doi:10.3390/plants9111526 2223-7747 https://doaj.org/article/fe2c27581c954525863022458db57f9f Plants, Vol 9, Iss 1526, p 1526 (2020) micropropagation psammophytes halophytes topophysis salinity position of explants Botany QK1-989 article 2020 ftdoajarticles https://doi.org/10.3390/plants9111526 2022-12-31T15:10:01Z The sea sandwort— Honckenya peploides (L.) Ehrh. is—a rare halophilous plant growing on dunes and is an endangered species on the Polish coast. It contributes to the stabilization of volatile sandy substrate, facilitating the colonization of other species. The present study determined the reaction of two types of explant: apical shoot fragments and fragments from a lower portion of the shoot. Apical shoot fragments were used to propagate and root sea sandwort plants due to the positive impact on the development of shoots and roots. Regardless of the plant growth regulators applied in the medium, the lateral meristems on the explants from the lower parts of the shoot stopped growing, and then yellowed and died out. Apical fragments of shoots developed higher and more numerous shoots and longer and more numerous roots than explants, which were fragments collected from lower parts of shoots. The findings indicated that propagation should be conducted on Murashige and Skoog medium with the addition of 1 mg∙dm −3 kinetin, whereas shoots with their apical fragments should be rooted with the addition of 1.5 mg∙dm −3 1-naphthaleneacetic acid. The results also showed that the addition of NaCl at concentrations of 25 and 50 mM did not restrict their growth, thereby indicating the tolerance of the plant to soil salinity. However, an increase in the concentration of NaCl in the medium to 75 mM restricted the development of plants, and the shoots were lower and roots were shorter and less numerous. Article in Journal/Newspaper Honckenya peploides Directory of Open Access Journals: DOAJ Articles Plants 9 11 1526 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
micropropagation psammophytes halophytes topophysis salinity position of explants Botany QK1-989 |
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micropropagation psammophytes halophytes topophysis salinity position of explants Botany QK1-989 Danuta Kulpa Mariola Wrobel Martyna Bednarek Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh |
topic_facet |
micropropagation psammophytes halophytes topophysis salinity position of explants Botany QK1-989 |
description |
The sea sandwort— Honckenya peploides (L.) Ehrh. is—a rare halophilous plant growing on dunes and is an endangered species on the Polish coast. It contributes to the stabilization of volatile sandy substrate, facilitating the colonization of other species. The present study determined the reaction of two types of explant: apical shoot fragments and fragments from a lower portion of the shoot. Apical shoot fragments were used to propagate and root sea sandwort plants due to the positive impact on the development of shoots and roots. Regardless of the plant growth regulators applied in the medium, the lateral meristems on the explants from the lower parts of the shoot stopped growing, and then yellowed and died out. Apical fragments of shoots developed higher and more numerous shoots and longer and more numerous roots than explants, which were fragments collected from lower parts of shoots. The findings indicated that propagation should be conducted on Murashige and Skoog medium with the addition of 1 mg∙dm −3 kinetin, whereas shoots with their apical fragments should be rooted with the addition of 1.5 mg∙dm −3 1-naphthaleneacetic acid. The results also showed that the addition of NaCl at concentrations of 25 and 50 mM did not restrict their growth, thereby indicating the tolerance of the plant to soil salinity. However, an increase in the concentration of NaCl in the medium to 75 mM restricted the development of plants, and the shoots were lower and roots were shorter and less numerous. |
format |
Article in Journal/Newspaper |
author |
Danuta Kulpa Mariola Wrobel Martyna Bednarek |
author_facet |
Danuta Kulpa Mariola Wrobel Martyna Bednarek |
author_sort |
Danuta Kulpa |
title |
Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh |
title_short |
Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh |
title_full |
Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh |
title_fullStr |
Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh |
title_full_unstemmed |
Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh |
title_sort |
type of explant affects in vitro development and multiplication success of the rare halophyte plant honckenya peploides l. ehrh |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/plants9111526 https://doaj.org/article/fe2c27581c954525863022458db57f9f |
genre |
Honckenya peploides |
genre_facet |
Honckenya peploides |
op_source |
Plants, Vol 9, Iss 1526, p 1526 (2020) |
op_relation |
https://www.mdpi.com/2223-7747/9/11/1526 https://doaj.org/toc/2223-7747 doi:10.3390/plants9111526 2223-7747 https://doaj.org/article/fe2c27581c954525863022458db57f9f |
op_doi |
https://doi.org/10.3390/plants9111526 |
container_title |
Plants |
container_volume |
9 |
container_issue |
11 |
container_start_page |
1526 |
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1766024825338331136 |