ACTIVITY OF LYSOSOMAL PROTEASES (CATHEPSINS B AND D) IN THE MUSCLES OF JUVENILE (0+, 1+ 2+) ATLANTIC SALMON FROM VARZUGA RIVER
The results of experimental studies on changes in lysosomal protease activity in muscles of Atlantic salmon on the early stages of ontogeny are presented in the article. The main lysosomal proteolytic enzymes (cathepsins B and D) participated in lysosomal autophagic cell system and varied in chemica...
Published in: | Proceedings of the Karelian Research Centre of the Russian Academy of Sciences |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English Russian |
Published: |
Karelian Research Centre of the Russian Academy of Sciences
2015
|
Subjects: | |
Online Access: | https://doi.org/10.17076/eb237 https://doaj.org/article/fdf3d6c8778143a697d7063b493ccd5f |
id |
ftdoajarticles:oai:doaj.org/article:fdf3d6c8778143a697d7063b493ccd5f |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:fdf3d6c8778143a697d7063b493ccd5f 2023-05-15T15:30:08+02:00 ACTIVITY OF LYSOSOMAL PROTEASES (CATHEPSINS B AND D) IN THE MUSCLES OF JUVENILE (0+, 1+ 2+) ATLANTIC SALMON FROM VARZUGA RIVER Nina Nemova Marina Krupnova Denis Efremov Aleksey Veselov 2015-12-01T00:00:00Z https://doi.org/10.17076/eb237 https://doaj.org/article/fdf3d6c8778143a697d7063b493ccd5f EN RU eng rus Karelian Research Centre of the Russian Academy of Sciences http://journals.krc.karelia.ru/index.php/biology/article/view/237 https://doaj.org/toc/1997-3217 https://doaj.org/toc/2312-4504 1997-3217 2312-4504 doi:10.17076/eb237 https://doaj.org/article/fdf3d6c8778143a697d7063b493ccd5f Transactions of the Karelian Research Centre of the Russian Academy of Sciences, Iss 11, Pp 85-91 (2015) environmental biochemistry atlantic salmon early development lysosomes cathepsins Science Q article 2015 ftdoajarticles https://doi.org/10.17076/eb237 2022-12-30T22:17:24Z The results of experimental studies on changes in lysosomal protease activity in muscles of Atlantic salmon on the early stages of ontogeny are presented in the article. The main lysosomal proteolytic enzymes (cathepsins B and D) participated in lysosomal autophagic cell system and varied in chemical composition, catalysis, pH optimum, substrate specificity, with respect to ingibitors, functional activity were investigated. A relatively high activity of the main lysosomal aspartate type endoproteinase (cathepsin D) on the early stage of young’s-of-the-year salmon (0+) was shown. It indicates the leading role of the enzyme in complete protein degradation required to supply with amino acids and peptides to maintain immediately the homeostasis in juveniles. The activity of lysosomal cystein type proteinase (cathepsin B) increases as the development of salmon progresses from the stage 0+ (fingerlings) to stage 2+ (parr). It shows the intensification of proteolysis due to increase of growth rate. Multidirectional change in the activity of the main lysosomal proteases (increase in the activity of cathepsin B and decrease in the activity of cathepsin D) in the muscles of Atlantic salmon of ages 0+, 1+, 2+ indicates the stage-specific character of the involvement of hydrolases studied in intracellular proteolysis of juvenile salmon. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Varzuga ENVELOPE(36.942,36.942,66.268,66.268) Proceedings of the Karelian Research Centre of the Russian Academy of Sciences 11 85 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Russian |
topic |
environmental biochemistry atlantic salmon early development lysosomes cathepsins Science Q |
spellingShingle |
environmental biochemistry atlantic salmon early development lysosomes cathepsins Science Q Nina Nemova Marina Krupnova Denis Efremov Aleksey Veselov ACTIVITY OF LYSOSOMAL PROTEASES (CATHEPSINS B AND D) IN THE MUSCLES OF JUVENILE (0+, 1+ 2+) ATLANTIC SALMON FROM VARZUGA RIVER |
topic_facet |
environmental biochemistry atlantic salmon early development lysosomes cathepsins Science Q |
description |
The results of experimental studies on changes in lysosomal protease activity in muscles of Atlantic salmon on the early stages of ontogeny are presented in the article. The main lysosomal proteolytic enzymes (cathepsins B and D) participated in lysosomal autophagic cell system and varied in chemical composition, catalysis, pH optimum, substrate specificity, with respect to ingibitors, functional activity were investigated. A relatively high activity of the main lysosomal aspartate type endoproteinase (cathepsin D) on the early stage of young’s-of-the-year salmon (0+) was shown. It indicates the leading role of the enzyme in complete protein degradation required to supply with amino acids and peptides to maintain immediately the homeostasis in juveniles. The activity of lysosomal cystein type proteinase (cathepsin B) increases as the development of salmon progresses from the stage 0+ (fingerlings) to stage 2+ (parr). It shows the intensification of proteolysis due to increase of growth rate. Multidirectional change in the activity of the main lysosomal proteases (increase in the activity of cathepsin B and decrease in the activity of cathepsin D) in the muscles of Atlantic salmon of ages 0+, 1+, 2+ indicates the stage-specific character of the involvement of hydrolases studied in intracellular proteolysis of juvenile salmon. |
format |
Article in Journal/Newspaper |
author |
Nina Nemova Marina Krupnova Denis Efremov Aleksey Veselov |
author_facet |
Nina Nemova Marina Krupnova Denis Efremov Aleksey Veselov |
author_sort |
Nina Nemova |
title |
ACTIVITY OF LYSOSOMAL PROTEASES (CATHEPSINS B AND D) IN THE MUSCLES OF JUVENILE (0+, 1+ 2+) ATLANTIC SALMON FROM VARZUGA RIVER |
title_short |
ACTIVITY OF LYSOSOMAL PROTEASES (CATHEPSINS B AND D) IN THE MUSCLES OF JUVENILE (0+, 1+ 2+) ATLANTIC SALMON FROM VARZUGA RIVER |
title_full |
ACTIVITY OF LYSOSOMAL PROTEASES (CATHEPSINS B AND D) IN THE MUSCLES OF JUVENILE (0+, 1+ 2+) ATLANTIC SALMON FROM VARZUGA RIVER |
title_fullStr |
ACTIVITY OF LYSOSOMAL PROTEASES (CATHEPSINS B AND D) IN THE MUSCLES OF JUVENILE (0+, 1+ 2+) ATLANTIC SALMON FROM VARZUGA RIVER |
title_full_unstemmed |
ACTIVITY OF LYSOSOMAL PROTEASES (CATHEPSINS B AND D) IN THE MUSCLES OF JUVENILE (0+, 1+ 2+) ATLANTIC SALMON FROM VARZUGA RIVER |
title_sort |
activity of lysosomal proteases (cathepsins b and d) in the muscles of juvenile (0+, 1+ 2+) atlantic salmon from varzuga river |
publisher |
Karelian Research Centre of the Russian Academy of Sciences |
publishDate |
2015 |
url |
https://doi.org/10.17076/eb237 https://doaj.org/article/fdf3d6c8778143a697d7063b493ccd5f |
long_lat |
ENVELOPE(36.942,36.942,66.268,66.268) |
geographic |
Varzuga |
geographic_facet |
Varzuga |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Transactions of the Karelian Research Centre of the Russian Academy of Sciences, Iss 11, Pp 85-91 (2015) |
op_relation |
http://journals.krc.karelia.ru/index.php/biology/article/view/237 https://doaj.org/toc/1997-3217 https://doaj.org/toc/2312-4504 1997-3217 2312-4504 doi:10.17076/eb237 https://doaj.org/article/fdf3d6c8778143a697d7063b493ccd5f |
op_doi |
https://doi.org/10.17076/eb237 |
container_title |
Proceedings of the Karelian Research Centre of the Russian Academy of Sciences |
container_issue |
11 |
container_start_page |
85 |
_version_ |
1766360584611168256 |