Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.
Background Endomyocardial Fibrosis (EMF) is a tropical restrictive cardiomyopathy of unknown etiology with high prevalence in Sub-Saharan Africa, for which it is unclear whether the primary target of injury is the endocardial endothelium, the subendocardial fibroblast, the coronary microcirculation...
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ftdoajarticles:oai:doaj.org/article:fdc723fb100f4affa50a319c0a936e36 2023-05-15T15:12:55+02:00 Presence of circulating anti-myosin antibodies in endomyocardial fibrosis. Ana Olga Mocumbi Najma Latif Magdi H Yacoub 2010-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000661 https://doaj.org/article/fdc723fb100f4affa50a319c0a936e36 EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20422043/pdf/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000661 https://doaj.org/article/fdc723fb100f4affa50a319c0a936e36 PLoS Neglected Tropical Diseases, Vol 4, Iss 4, p e661 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000661 2022-12-31T08:59:20Z Background Endomyocardial Fibrosis (EMF) is a tropical restrictive cardiomyopathy of unknown etiology with high prevalence in Sub-Saharan Africa, for which it is unclear whether the primary target of injury is the endocardial endothelium, the subendocardial fibroblast, the coronary microcirculation or the myocyte. In an attempt to explore the possibility of endocardial lesions being a result of an immune response against the myocyte we assessed the presence and frequency of circulating anti-myocardial antibodies in EMF patients. Methodology/principal findings EMF classification, assessment of severity and staging was based on echocardiography. We used sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) of myocardial proteins followed by western blotting to screen serum samples for antiheart antibodies G and M classes. The degree of serum reactivity was correlated with the severity and activity of EMF. We studied 56 EMF patients and 10 healthy controls. IgG reactivity against myocardial proteins was stronger and more frequent in patients with EMF when compared to controls (30/56; 53.6% vs. 1/10; 10%, respectively). IgM reactivity was weak in both groups, although higher in EMF patients (11/56; 19.6%) when compared to controls (n = 0). EMF patients showed greater frequency and reactivity of IgG antibodies against myocardial proteins of molecular weights 35 kD, 42 kD and 70 kD (p values <0.01, <0.01 and <0.05 respectively). Conclusions The presence of antibodies against myocardial proteins was demonstrated in a subset of EMF patients. These immune markers seem to be related with activity and might provide an adjunct tool for diagnosis and classification of EMF, therefore improving its management by identifying patients who may benefit from immunosuppressive therapy. Further research is needed to clarify the role of autoimmunity in the pathogenesis of EMF. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 4 e661 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ana Olga Mocumbi Najma Latif Magdi H Yacoub Presence of circulating anti-myosin antibodies in endomyocardial fibrosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Endomyocardial Fibrosis (EMF) is a tropical restrictive cardiomyopathy of unknown etiology with high prevalence in Sub-Saharan Africa, for which it is unclear whether the primary target of injury is the endocardial endothelium, the subendocardial fibroblast, the coronary microcirculation or the myocyte. In an attempt to explore the possibility of endocardial lesions being a result of an immune response against the myocyte we assessed the presence and frequency of circulating anti-myocardial antibodies in EMF patients. Methodology/principal findings EMF classification, assessment of severity and staging was based on echocardiography. We used sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) of myocardial proteins followed by western blotting to screen serum samples for antiheart antibodies G and M classes. The degree of serum reactivity was correlated with the severity and activity of EMF. We studied 56 EMF patients and 10 healthy controls. IgG reactivity against myocardial proteins was stronger and more frequent in patients with EMF when compared to controls (30/56; 53.6% vs. 1/10; 10%, respectively). IgM reactivity was weak in both groups, although higher in EMF patients (11/56; 19.6%) when compared to controls (n = 0). EMF patients showed greater frequency and reactivity of IgG antibodies against myocardial proteins of molecular weights 35 kD, 42 kD and 70 kD (p values <0.01, <0.01 and <0.05 respectively). Conclusions The presence of antibodies against myocardial proteins was demonstrated in a subset of EMF patients. These immune markers seem to be related with activity and might provide an adjunct tool for diagnosis and classification of EMF, therefore improving its management by identifying patients who may benefit from immunosuppressive therapy. Further research is needed to clarify the role of autoimmunity in the pathogenesis of EMF. |
format |
Article in Journal/Newspaper |
author |
Ana Olga Mocumbi Najma Latif Magdi H Yacoub |
author_facet |
Ana Olga Mocumbi Najma Latif Magdi H Yacoub |
author_sort |
Ana Olga Mocumbi |
title |
Presence of circulating anti-myosin antibodies in endomyocardial fibrosis. |
title_short |
Presence of circulating anti-myosin antibodies in endomyocardial fibrosis. |
title_full |
Presence of circulating anti-myosin antibodies in endomyocardial fibrosis. |
title_fullStr |
Presence of circulating anti-myosin antibodies in endomyocardial fibrosis. |
title_full_unstemmed |
Presence of circulating anti-myosin antibodies in endomyocardial fibrosis. |
title_sort |
presence of circulating anti-myosin antibodies in endomyocardial fibrosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000661 https://doaj.org/article/fdc723fb100f4affa50a319c0a936e36 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 4, p e661 (2010) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20422043/pdf/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000661 https://doaj.org/article/fdc723fb100f4affa50a319c0a936e36 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000661 |
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PLoS Neglected Tropical Diseases |
container_volume |
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4 |
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e661 |
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