Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area
Abstract Background Sardinia is a Mediterranean area endemic for malaria up to the last century. During a screening study to evaluate the anti-plasmodial activity of some aromatic plants traditionally used in Sardinia, Myrtus communis (myrtle, Myrtaceae), Satureja thymbra (savory, Lamiaceae), and Th...
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ftdoajarticles:oai:doaj.org/article:fd8febdd9d754684830fa0e5b0d619b9 2023-05-15T15:17:55+02:00 Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area Dell’Agli Mario Sanna Cinzia Rubiolo Patrizia Basilico Nicoletta Colombo Elisa Scaltrito Maria M Ndiath Mamadou Maccarone Luca Taramelli Donatella Bicchi Carlo Ballero Mauro Bosisio Enrica 2012-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-219 https://doaj.org/article/fd8febdd9d754684830fa0e5b0d619b9 EN eng BMC http://www.malariajournal.com/content/11/1/219 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-219 1475-2875 https://doaj.org/article/fd8febdd9d754684830fa0e5b0d619b9 Malaria Journal, Vol 11, Iss 1, p 219 (2012) Essential oil Insecticidal activity Plasmodium falciparum Satureja thymbra Myrtus communis Thymus herba-barona Thymol Anopheles gambiae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-219 2022-12-31T02:54:35Z Abstract Background Sardinia is a Mediterranean area endemic for malaria up to the last century. During a screening study to evaluate the anti-plasmodial activity of some aromatic plants traditionally used in Sardinia, Myrtus communis (myrtle, Myrtaceae), Satureja thymbra (savory, Lamiaceae), and Thymus herba-barona (caraway thyme, Lamiaceae) were collected in three vegetative periods: before, during and after flowering. Methods The essential oils were obtained by steam distillation, fractionated by silica gel column chromatography and analysed by GC-FID-MS. Total oil and three main fractions were tested on D10 and W2 strains of Plasmodium falciparum in vitro . Larvicidal and adulticidal activities were tested on Anopheles gambiae susceptible strains. Results The essential oil of savory, rich in thymol, was the most effective against P. falciparum with an inhibitory activity independent from the time of collection (IC 50 17–26 μg/ml on D10 and 9–11 μg/ml on W2). Upon fractionation, fraction 1 was enriched in mono-sesquiterpenoid hydrocarbons; fraction 2 in thymol (73-83%); and fraction 3 contained thymol, carvacrol and terpinen-4-ol, with a different composition depending on the time of collection. Thymol-enriched fractions were the most active on both strains (IC 50 20–22 μg/ml on D10 and 8–10 μg/ml on W2) and thymol was confirmed as mainly responsible for this activity (IC 50 19.7± 3.0 and 10.6 ± 2.0 μg/ml on D10 and W2, respectively). The essential oil of S. thymbra L. showed also larvicidal and adulticidal activities. The larvicidal activity, expressed as LC 50 , was 0.15 ± 0.002; 0.21 ± 0.13; and 0.15 ± 0.09 μg/ml (mean ± sd) depending on the time of collection: before, during and after flowering, respectively. Conclusions This study provides evidence for the use of essential oils for treating malaria and fighting the vector at both the larval and adult stages. These findings open the possibility for further investigation aimed at the isolation of natural products with anti-parasitic properties. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Fid ENVELOPE(-65.939,-65.939,-68.664,-68.664) Savory ENVELOPE(-125.170,-125.170,54.100,54.100) Malaria Journal 11 1 219 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Essential oil Insecticidal activity Plasmodium falciparum Satureja thymbra Myrtus communis Thymus herba-barona Thymol Anopheles gambiae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Essential oil Insecticidal activity Plasmodium falciparum Satureja thymbra Myrtus communis Thymus herba-barona Thymol Anopheles gambiae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Dell’Agli Mario Sanna Cinzia Rubiolo Patrizia Basilico Nicoletta Colombo Elisa Scaltrito Maria M Ndiath Mamadou Maccarone Luca Taramelli Donatella Bicchi Carlo Ballero Mauro Bosisio Enrica Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area |
topic_facet |
Essential oil Insecticidal activity Plasmodium falciparum Satureja thymbra Myrtus communis Thymus herba-barona Thymol Anopheles gambiae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Sardinia is a Mediterranean area endemic for malaria up to the last century. During a screening study to evaluate the anti-plasmodial activity of some aromatic plants traditionally used in Sardinia, Myrtus communis (myrtle, Myrtaceae), Satureja thymbra (savory, Lamiaceae), and Thymus herba-barona (caraway thyme, Lamiaceae) were collected in three vegetative periods: before, during and after flowering. Methods The essential oils were obtained by steam distillation, fractionated by silica gel column chromatography and analysed by GC-FID-MS. Total oil and three main fractions were tested on D10 and W2 strains of Plasmodium falciparum in vitro . Larvicidal and adulticidal activities were tested on Anopheles gambiae susceptible strains. Results The essential oil of savory, rich in thymol, was the most effective against P. falciparum with an inhibitory activity independent from the time of collection (IC 50 17–26 μg/ml on D10 and 9–11 μg/ml on W2). Upon fractionation, fraction 1 was enriched in mono-sesquiterpenoid hydrocarbons; fraction 2 in thymol (73-83%); and fraction 3 contained thymol, carvacrol and terpinen-4-ol, with a different composition depending on the time of collection. Thymol-enriched fractions were the most active on both strains (IC 50 20–22 μg/ml on D10 and 8–10 μg/ml on W2) and thymol was confirmed as mainly responsible for this activity (IC 50 19.7± 3.0 and 10.6 ± 2.0 μg/ml on D10 and W2, respectively). The essential oil of S. thymbra L. showed also larvicidal and adulticidal activities. The larvicidal activity, expressed as LC 50 , was 0.15 ± 0.002; 0.21 ± 0.13; and 0.15 ± 0.09 μg/ml (mean ± sd) depending on the time of collection: before, during and after flowering, respectively. Conclusions This study provides evidence for the use of essential oils for treating malaria and fighting the vector at both the larval and adult stages. These findings open the possibility for further investigation aimed at the isolation of natural products with anti-parasitic properties. |
format |
Article in Journal/Newspaper |
author |
Dell’Agli Mario Sanna Cinzia Rubiolo Patrizia Basilico Nicoletta Colombo Elisa Scaltrito Maria M Ndiath Mamadou Maccarone Luca Taramelli Donatella Bicchi Carlo Ballero Mauro Bosisio Enrica |
author_facet |
Dell’Agli Mario Sanna Cinzia Rubiolo Patrizia Basilico Nicoletta Colombo Elisa Scaltrito Maria M Ndiath Mamadou Maccarone Luca Taramelli Donatella Bicchi Carlo Ballero Mauro Bosisio Enrica |
author_sort |
Dell’Agli Mario |
title |
Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area |
title_short |
Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area |
title_full |
Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area |
title_fullStr |
Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area |
title_full_unstemmed |
Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area |
title_sort |
anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the mediterranean area |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-219 https://doaj.org/article/fd8febdd9d754684830fa0e5b0d619b9 |
long_lat |
ENVELOPE(-65.939,-65.939,-68.664,-68.664) ENVELOPE(-125.170,-125.170,54.100,54.100) |
geographic |
Arctic Fid Savory |
geographic_facet |
Arctic Fid Savory |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 219 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/219 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-219 1475-2875 https://doaj.org/article/fd8febdd9d754684830fa0e5b0d619b9 |
op_doi |
https://doi.org/10.1186/1475-2875-11-219 |
container_title |
Malaria Journal |
container_volume |
11 |
container_issue |
1 |
container_start_page |
219 |
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1766348176051142656 |