Entamoeba histolytica: antigenic characterization of axenic strains from Brazil Entamoeba histolytica: caracterização antigênica de cepas axênicas do Brasil

Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain iso...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Semíramis Guimarães, Haidée Urdaneta, Edward F. Silva, Carlos Alberto P. Tavares
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1991
Subjects:
Entamoeba histolytica
Axenic strains
Antigens
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46651991000100002
https://doaj.org/article/fd8606e75f78469899581ca6f1c6ef42
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Summary:Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains. Trofozoítos obtidos de cultura de Entamoeba histolytica isoladas e axenizadas no Brasil (ICB-CSP, ICB-462 e ICB-32) foram utilizados para a produção de soros imunes em coelhos e para a caracterização de antígenos através dos seus perfis eletroforético e glicoproteico, em paralelo com uma cepa padrão isolada e axenizada nos Estados Unidos (HK-9). Obtiveram-se soros hiperimunes, reativos frente aos antígenos homólogos e heterólogos. As quatro cepas em estudo apresentaram perfis eletroforético e glicoproteico complexos e semelhantes, compostos por polipeptídeos com pesos moleculares variando de 200 kDa a menos de 29 kDa. Não se detectaram diferenças significativas entre as cepas patogênicas e não patogênicas.

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