Analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition
The composition of the microbiome influences many aspects of physiology and health, and can be altered by environmental factors, including diet and activity. Glucosamine is a dietary supplement often administered to address arthritic symptoms in humans, dogs, and other mammals. To investigate how gu...
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ftdoajarticles:oai:doaj.org/article:fd818249dfd3455fb4d8ee8d7702369d 2024-09-15T18:15:04+00:00 Analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition Dong Wang William A. Russel Kaitlyn M. Macdonald Valerie M. De Leon Ahmet Ay Kenneth D. Belanger 2024-02-01T00:00:00Z https://doi.org/10.3389/fvets.2024.1272711 https://doaj.org/article/fd818249dfd3455fb4d8ee8d7702369d EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fvets.2024.1272711/full https://doaj.org/toc/2297-1769 2297-1769 doi:10.3389/fvets.2024.1272711 https://doaj.org/article/fd818249dfd3455fb4d8ee8d7702369d Frontiers in Veterinary Science, Vol 11 (2024) microbiome glucosamine cosequin canine health gut health microbial diversity Veterinary medicine SF600-1100 article 2024 ftdoajarticles https://doi.org/10.3389/fvets.2024.1272711 2024-08-05T17:50:05Z The composition of the microbiome influences many aspects of physiology and health, and can be altered by environmental factors, including diet and activity. Glucosamine is a dietary supplement often administered to address arthritic symptoms in humans, dogs, and other mammals. To investigate how gut microbial composition varies with glucosamine supplementation, we performed 16S rRNA sequence analysis of fecal samples from 24 Alaskan and Inuit huskies and used mixed effects models to investigate associations with activity, age, and additional factors. Glucosamine ingestion, age, activity, sex, and diet were correlated with differences in alpha-diversity, with diversity decreasing in dogs consuming glucosamine. Beta-diversity analysis revealed clustering of dogs based on glucosamine supplementation status. Glucosamine supplementation and exercise-related activity were associated with greater inter-individual pairwise distances. At the family level, Lactobacillaceae and Anaerovoracaceae relative abundances were lower in supplemented dogs when activity was accounted for. At the genus level, Eubacterium [brachy], Sellimonus, Parvibacter, and an unclassified genus belonging to the same family as Parvibacter (Eggerthellaceae) all were lower in supplemented dogs, but only significantly so post-activity. Our findings suggest that glucosamine supplementation alters microbiome composition in sled dogs, particularly in the context of exercise-related activity. Article in Journal/Newspaper inuit Huskies Directory of Open Access Journals: DOAJ Articles Frontiers in Veterinary Science 11 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
microbiome glucosamine cosequin canine health gut health microbial diversity Veterinary medicine SF600-1100 |
spellingShingle |
microbiome glucosamine cosequin canine health gut health microbial diversity Veterinary medicine SF600-1100 Dong Wang William A. Russel Kaitlyn M. Macdonald Valerie M. De Leon Ahmet Ay Kenneth D. Belanger Analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition |
topic_facet |
microbiome glucosamine cosequin canine health gut health microbial diversity Veterinary medicine SF600-1100 |
description |
The composition of the microbiome influences many aspects of physiology and health, and can be altered by environmental factors, including diet and activity. Glucosamine is a dietary supplement often administered to address arthritic symptoms in humans, dogs, and other mammals. To investigate how gut microbial composition varies with glucosamine supplementation, we performed 16S rRNA sequence analysis of fecal samples from 24 Alaskan and Inuit huskies and used mixed effects models to investigate associations with activity, age, and additional factors. Glucosamine ingestion, age, activity, sex, and diet were correlated with differences in alpha-diversity, with diversity decreasing in dogs consuming glucosamine. Beta-diversity analysis revealed clustering of dogs based on glucosamine supplementation status. Glucosamine supplementation and exercise-related activity were associated with greater inter-individual pairwise distances. At the family level, Lactobacillaceae and Anaerovoracaceae relative abundances were lower in supplemented dogs when activity was accounted for. At the genus level, Eubacterium [brachy], Sellimonus, Parvibacter, and an unclassified genus belonging to the same family as Parvibacter (Eggerthellaceae) all were lower in supplemented dogs, but only significantly so post-activity. Our findings suggest that glucosamine supplementation alters microbiome composition in sled dogs, particularly in the context of exercise-related activity. |
format |
Article in Journal/Newspaper |
author |
Dong Wang William A. Russel Kaitlyn M. Macdonald Valerie M. De Leon Ahmet Ay Kenneth D. Belanger |
author_facet |
Dong Wang William A. Russel Kaitlyn M. Macdonald Valerie M. De Leon Ahmet Ay Kenneth D. Belanger |
author_sort |
Dong Wang |
title |
Analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition |
title_short |
Analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition |
title_full |
Analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition |
title_fullStr |
Analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition |
title_full_unstemmed |
Analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition |
title_sort |
analysis of the gut microbiome in sled dogs reveals glucosamine- and activity-related effects on gut microbial composition |
publisher |
Frontiers Media S.A. |
publishDate |
2024 |
url |
https://doi.org/10.3389/fvets.2024.1272711 https://doaj.org/article/fd818249dfd3455fb4d8ee8d7702369d |
genre |
inuit Huskies |
genre_facet |
inuit Huskies |
op_source |
Frontiers in Veterinary Science, Vol 11 (2024) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fvets.2024.1272711/full https://doaj.org/toc/2297-1769 2297-1769 doi:10.3389/fvets.2024.1272711 https://doaj.org/article/fd818249dfd3455fb4d8ee8d7702369d |
op_doi |
https://doi.org/10.3389/fvets.2024.1272711 |
container_title |
Frontiers in Veterinary Science |
container_volume |
11 |
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1810452813588725760 |