Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways
Clostridium autoethanogenum protein (CAP) is a promising protein source for aquaculture; however, how CAP influences fish quality is worth extensive research. We randomly allocated 630 turbot with initial body weights of about 180 g into 6 groups, with fishmeal-based control diet or diet with CAP re...
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KeAi Communications Co., Ltd.
2024
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Online Access: | https://doi.org/10.1016/j.aninu.2024.04.012 https://doaj.org/article/fd7df292fd814337882b4faf62ff2238 |
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ftdoajarticles:oai:doaj.org/article:fd7df292fd814337882b4faf62ff2238 2024-09-15T18:34:02+00:00 Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways Zezheng Qi Nan Bai Qing Li Shihui Pan Min Gu 2024-09-01T00:00:00Z https://doi.org/10.1016/j.aninu.2024.04.012 https://doaj.org/article/fd7df292fd814337882b4faf62ff2238 EN eng KeAi Communications Co., Ltd. http://www.sciencedirect.com/science/article/pii/S2405654524000623 https://doaj.org/toc/2405-6545 2405-6545 doi:10.1016/j.aninu.2024.04.012 https://doaj.org/article/fd7df292fd814337882b4faf62ff2238 Animal Nutrition, Vol 18, Iss , Pp 84-95 (2024) Bacterial meal Clostridium autoethanogenum Turbot Fish quality Muscle growth Animal culture SF1-1100 article 2024 ftdoajarticles https://doi.org/10.1016/j.aninu.2024.04.012 2024-08-05T17:49:00Z Clostridium autoethanogenum protein (CAP) is a promising protein source for aquaculture; however, how CAP influences fish quality is worth extensive research. We randomly allocated 630 turbot with initial body weights of about 180 g into 6 groups, with fishmeal-based control diet or diet with CAP replacing 15% (CAP15), 30% (CAP30), 45% (CAP45), 60% (CAP60), or 75% (CAP75) of fishmeal protein. After a 70-d feeding trial, the fillet yield (P = 0.015) and content of protein (P = 0.017), collagen (P < 0.001), hydroxyproline (P < 0.001), C20:5n-3 (P = 0.007), and ∑n-3/∑n-6 polyunsaturated fatty acids ratio (P < 0.001) in turbot muscle was found to decrease linearly with increasing CAP. However, turbot fed CAP15 diet maintained these parameters (P > 0.05). By contrast, the muscle hardness increased linearly with increasing CAP (P = 0.004), accompanied by linear reduction of muscle fiber area (P = 0.003) and expression of myogenesis-related genes, including cathepsin D (ctsd P < 0.001) and muscle ring finger protein 1 (murf 1, P < 0.001). Phosphorylation of protein kinase B (Akt, P < 0.001), target of rapamycin (TOR, P = 0.001), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1, P < 0.001), and ribosomal protein S6 (S6, P < 0.001) decreased linearly; however, phosphorylation of AMP-activated protein kinase (AMPK, P < 0.001), eukaryotic initiation factor 2α (eIF2α, P < 0.001), and the abundance of activating transcription factor 4 (ATF4, P < 0.001) increased with increasing CAP, suggesting that the TOR signaling pathway was inhibited, and the amino acid response (AAR) and AMPK pathways were activated. Additionally, expression of genes related to protein degradation, including myogenic factor 5 (myf 5, P < 0.001), myogenic differentiation (myod, P < 0.001), paired box 7 (pax 7, P < 0.001), and ctsd (P < 0.001), decreased linearly with increasing CAP. In conclusion, CAP could be used to replace up to 15% of fishmeal without negatively impacting turbot quality. ... Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles Animal Nutrition 18 84 95 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bacterial meal Clostridium autoethanogenum Turbot Fish quality Muscle growth Animal culture SF1-1100 |
spellingShingle |
Bacterial meal Clostridium autoethanogenum Turbot Fish quality Muscle growth Animal culture SF1-1100 Zezheng Qi Nan Bai Qing Li Shihui Pan Min Gu Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways |
topic_facet |
Bacterial meal Clostridium autoethanogenum Turbot Fish quality Muscle growth Animal culture SF1-1100 |
description |
Clostridium autoethanogenum protein (CAP) is a promising protein source for aquaculture; however, how CAP influences fish quality is worth extensive research. We randomly allocated 630 turbot with initial body weights of about 180 g into 6 groups, with fishmeal-based control diet or diet with CAP replacing 15% (CAP15), 30% (CAP30), 45% (CAP45), 60% (CAP60), or 75% (CAP75) of fishmeal protein. After a 70-d feeding trial, the fillet yield (P = 0.015) and content of protein (P = 0.017), collagen (P < 0.001), hydroxyproline (P < 0.001), C20:5n-3 (P = 0.007), and ∑n-3/∑n-6 polyunsaturated fatty acids ratio (P < 0.001) in turbot muscle was found to decrease linearly with increasing CAP. However, turbot fed CAP15 diet maintained these parameters (P > 0.05). By contrast, the muscle hardness increased linearly with increasing CAP (P = 0.004), accompanied by linear reduction of muscle fiber area (P = 0.003) and expression of myogenesis-related genes, including cathepsin D (ctsd P < 0.001) and muscle ring finger protein 1 (murf 1, P < 0.001). Phosphorylation of protein kinase B (Akt, P < 0.001), target of rapamycin (TOR, P = 0.001), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1, P < 0.001), and ribosomal protein S6 (S6, P < 0.001) decreased linearly; however, phosphorylation of AMP-activated protein kinase (AMPK, P < 0.001), eukaryotic initiation factor 2α (eIF2α, P < 0.001), and the abundance of activating transcription factor 4 (ATF4, P < 0.001) increased with increasing CAP, suggesting that the TOR signaling pathway was inhibited, and the amino acid response (AAR) and AMPK pathways were activated. Additionally, expression of genes related to protein degradation, including myogenic factor 5 (myf 5, P < 0.001), myogenic differentiation (myod, P < 0.001), paired box 7 (pax 7, P < 0.001), and ctsd (P < 0.001), decreased linearly with increasing CAP. In conclusion, CAP could be used to replace up to 15% of fishmeal without negatively impacting turbot quality. ... |
format |
Article in Journal/Newspaper |
author |
Zezheng Qi Nan Bai Qing Li Shihui Pan Min Gu |
author_facet |
Zezheng Qi Nan Bai Qing Li Shihui Pan Min Gu |
author_sort |
Zezheng Qi |
title |
Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways |
title_short |
Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways |
title_full |
Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways |
title_fullStr |
Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways |
title_full_unstemmed |
Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways |
title_sort |
dietary fishmeal replacement by clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (scophthalmus maximus) via the tor/aar/ampk pathways |
publisher |
KeAi Communications Co., Ltd. |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.aninu.2024.04.012 https://doaj.org/article/fd7df292fd814337882b4faf62ff2238 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Animal Nutrition, Vol 18, Iss , Pp 84-95 (2024) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2405654524000623 https://doaj.org/toc/2405-6545 2405-6545 doi:10.1016/j.aninu.2024.04.012 https://doaj.org/article/fd7df292fd814337882b4faf62ff2238 |
op_doi |
https://doi.org/10.1016/j.aninu.2024.04.012 |
container_title |
Animal Nutrition |
container_volume |
18 |
container_start_page |
84 |
op_container_end_page |
95 |
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1810475759874080768 |