Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2
Abstract Background Rapid diagnostic test (RDT) positivity is supplanting microscopy as the standard measure of malaria burden at the population level. However, there is currently no standard for externally validating RDT results from field surveys. Methods Individuals’ blood concentration of the Pl...
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ftdoajarticles:oai:doaj.org/article:fd680535f48e4ecca601f4085356b3ae 2023-05-15T15:10:00+02:00 Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2 Mateusz Plucinski Rafael Dimbu Baltazar Candrinho James Colborn Aida Badiane Daouda Ndiaye Kimberly Mace Michelle Chang Jean F. Lemoine Eric S. Halsey John W. Barnwell Venkatachalam Udhayakumar Michael Aidoo Eric Rogier 2017-11-01T00:00:00Z https://doi.org/10.1186/s12936-017-2101-8 https://doaj.org/article/fd680535f48e4ecca601f4085356b3ae EN eng BMC http://link.springer.com/article/10.1186/s12936-017-2101-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2101-8 1475-2875 https://doaj.org/article/fd680535f48e4ecca601f4085356b3ae Malaria Journal, Vol 16, Iss 1, Pp 1-7 (2017) Malaria Rapid diagnostic test Limit of detection Bead assay Histidine-rich protein 2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-2101-8 2022-12-31T14:21:29Z Abstract Background Rapid diagnostic test (RDT) positivity is supplanting microscopy as the standard measure of malaria burden at the population level. However, there is currently no standard for externally validating RDT results from field surveys. Methods Individuals’ blood concentration of the Plasmodium falciparum histidine rich protein 2 (HRP2) protein were compared to results of HRP2-detecting RDTs in participants from field surveys in Angola, Mozambique, Haiti, and Senegal. A logistic regression model was used to estimate the HRP2 concentrations corresponding to the 50 and 90% level of detection (LOD) specific for each survey. Results There was a sigmoidal dose–response relationship between HRP2 concentration and RDT positivity for all surveys. Variation was noted in estimates for field RDT sensitivity, with the 50% LOD ranging between 0.076 and 6.1 ng/mL and the 90% LOD ranging between 1.1 and 53 ng/mL. Surveys conducted in two different provinces of Angola using the same brand of RDT and same study methodology showed a threefold difference in LOD. Conclusions Measures of malaria prevalence estimated using population RDT positivity should be interpreted in the context of potentially large variation in RDT LODs between, and even within, surveys. Surveys based on RDT positivity would benefit from external validation of field RDT results by comparing RDT positivity and antigen concentration. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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Malaria Rapid diagnostic test Limit of detection Bead assay Histidine-rich protein 2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Rapid diagnostic test Limit of detection Bead assay Histidine-rich protein 2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mateusz Plucinski Rafael Dimbu Baltazar Candrinho James Colborn Aida Badiane Daouda Ndiaye Kimberly Mace Michelle Chang Jean F. Lemoine Eric S. Halsey John W. Barnwell Venkatachalam Udhayakumar Michael Aidoo Eric Rogier Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2 |
topic_facet |
Malaria Rapid diagnostic test Limit of detection Bead assay Histidine-rich protein 2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Rapid diagnostic test (RDT) positivity is supplanting microscopy as the standard measure of malaria burden at the population level. However, there is currently no standard for externally validating RDT results from field surveys. Methods Individuals’ blood concentration of the Plasmodium falciparum histidine rich protein 2 (HRP2) protein were compared to results of HRP2-detecting RDTs in participants from field surveys in Angola, Mozambique, Haiti, and Senegal. A logistic regression model was used to estimate the HRP2 concentrations corresponding to the 50 and 90% level of detection (LOD) specific for each survey. Results There was a sigmoidal dose–response relationship between HRP2 concentration and RDT positivity for all surveys. Variation was noted in estimates for field RDT sensitivity, with the 50% LOD ranging between 0.076 and 6.1 ng/mL and the 90% LOD ranging between 1.1 and 53 ng/mL. Surveys conducted in two different provinces of Angola using the same brand of RDT and same study methodology showed a threefold difference in LOD. Conclusions Measures of malaria prevalence estimated using population RDT positivity should be interpreted in the context of potentially large variation in RDT LODs between, and even within, surveys. Surveys based on RDT positivity would benefit from external validation of field RDT results by comparing RDT positivity and antigen concentration. |
format |
Article in Journal/Newspaper |
author |
Mateusz Plucinski Rafael Dimbu Baltazar Candrinho James Colborn Aida Badiane Daouda Ndiaye Kimberly Mace Michelle Chang Jean F. Lemoine Eric S. Halsey John W. Barnwell Venkatachalam Udhayakumar Michael Aidoo Eric Rogier |
author_facet |
Mateusz Plucinski Rafael Dimbu Baltazar Candrinho James Colborn Aida Badiane Daouda Ndiaye Kimberly Mace Michelle Chang Jean F. Lemoine Eric S. Halsey John W. Barnwell Venkatachalam Udhayakumar Michael Aidoo Eric Rogier |
author_sort |
Mateusz Plucinski |
title |
Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2 |
title_short |
Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2 |
title_full |
Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2 |
title_fullStr |
Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2 |
title_full_unstemmed |
Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2 |
title_sort |
malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of plasmodium falciparum histidine-rich protein 2 |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-2101-8 https://doaj.org/article/fd680535f48e4ecca601f4085356b3ae |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-7 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-2101-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2101-8 1475-2875 https://doaj.org/article/fd680535f48e4ecca601f4085356b3ae |
op_doi |
https://doi.org/10.1186/s12936-017-2101-8 |
container_title |
Malaria Journal |
container_volume |
16 |
container_issue |
1 |
_version_ |
1766341078114369536 |