Optimization of antiscorpion venom production
The present study was carried out to produce highly efficient antivenom from a small number of telsons in a short time. Venom solution was prepared through maceration of telsons from Androctonus crassicauda (Olivier, 1807) collected in the Southeastern Anatolia Region, Turkey. Lethal dose 50% (LD50)...
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ftdoajarticles:oai:doaj.org/article:fd248fc24b8c4156afa0f6af8ad91127 2023-05-15T15:05:02+02:00 Optimization of antiscorpion venom production O. Ozkan S. Adigüzel C. Ates I. Bozyigit A. Filazi 2006-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992006000300004 https://doaj.org/article/fd248fc24b8c4156afa0f6af8ad91127 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992006000300004 1678-9199 https://doaj.org/article/fd248fc24b8c4156afa0f6af8ad91127 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 12, Iss 3, Pp 390-399 (2006) scorpion antivenom Androctonus crassicauda Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2006 ftdoajarticles https://doi.org/10.1590/S1678-91992006000300004 2022-12-31T04:29:45Z The present study was carried out to produce highly efficient antivenom from a small number of telsons in a short time. Venom solution was prepared through maceration of telsons from Androctonus crassicauda (Olivier, 1807) collected in the Southeastern Anatolia Region, Turkey. Lethal dose 50% (LD50) of the venom solution injected into mice was 1 ml/kg (95% confidence interval; 0.8-1.3), according to probit analysis. Different adjuvants (Freund's Complete Adjuvant, Freund's Incomplete Adjuvant, and 0.4% aluminium phosphate), at increasing doses and combined with venom, were subcutaneously injected into horses on days 0, 14, 21, 28, 35, and 42 of the experiment. Antivenom was collected from the immunized horses on days 45, 48, and 51 using the pepsin digestive method. The antivenom effective dose 50% (ED50) in mice was 0.5 ml (95% confidence interval; 0.40-0.58), according to probit analysis. It was concluded that 0.5 ml antivenom neutralized a venom dose 35-fold higher than the venom LD50. Thus, highly potent antivenom could be produced from about 238 telsons in 51 days. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 12 3 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
scorpion antivenom Androctonus crassicauda Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
scorpion antivenom Androctonus crassicauda Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 O. Ozkan S. Adigüzel C. Ates I. Bozyigit A. Filazi Optimization of antiscorpion venom production |
topic_facet |
scorpion antivenom Androctonus crassicauda Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The present study was carried out to produce highly efficient antivenom from a small number of telsons in a short time. Venom solution was prepared through maceration of telsons from Androctonus crassicauda (Olivier, 1807) collected in the Southeastern Anatolia Region, Turkey. Lethal dose 50% (LD50) of the venom solution injected into mice was 1 ml/kg (95% confidence interval; 0.8-1.3), according to probit analysis. Different adjuvants (Freund's Complete Adjuvant, Freund's Incomplete Adjuvant, and 0.4% aluminium phosphate), at increasing doses and combined with venom, were subcutaneously injected into horses on days 0, 14, 21, 28, 35, and 42 of the experiment. Antivenom was collected from the immunized horses on days 45, 48, and 51 using the pepsin digestive method. The antivenom effective dose 50% (ED50) in mice was 0.5 ml (95% confidence interval; 0.40-0.58), according to probit analysis. It was concluded that 0.5 ml antivenom neutralized a venom dose 35-fold higher than the venom LD50. Thus, highly potent antivenom could be produced from about 238 telsons in 51 days. |
format |
Article in Journal/Newspaper |
author |
O. Ozkan S. Adigüzel C. Ates I. Bozyigit A. Filazi |
author_facet |
O. Ozkan S. Adigüzel C. Ates I. Bozyigit A. Filazi |
author_sort |
O. Ozkan |
title |
Optimization of antiscorpion venom production |
title_short |
Optimization of antiscorpion venom production |
title_full |
Optimization of antiscorpion venom production |
title_fullStr |
Optimization of antiscorpion venom production |
title_full_unstemmed |
Optimization of antiscorpion venom production |
title_sort |
optimization of antiscorpion venom production |
publisher |
SciELO |
publishDate |
2006 |
url |
https://doi.org/10.1590/S1678-91992006000300004 https://doaj.org/article/fd248fc24b8c4156afa0f6af8ad91127 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 12, Iss 3, Pp 390-399 (2006) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992006000300004 1678-9199 https://doaj.org/article/fd248fc24b8c4156afa0f6af8ad91127 |
op_doi |
https://doi.org/10.1590/S1678-91992006000300004 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
12 |
container_issue |
3 |
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1766336807015809024 |