A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study.
Background Rapid diagnostic tests (RDTs) have become widely used in low-resource settings for leptospirosis diagnostic. This study aims to evaluate the diagnostic performance of the five commercially available RDTs to detect human IgM against Leptospira spp. in Thai population. Methodology/principal...
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ftdoajarticles:oai:doaj.org/article:fd1dd6a38e1f4832803f163472df7620 2023-05-15T15:07:28+02:00 A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study. Janejira Dinhuzen Umaporn Limothai Sasipha Tachaboon Panadda Krairojananan Bangon Laosatiankit Sakarin Boonprasong Nuttha Lumlertgul Sadudee Peerapornratana Nattachai Srisawat 2021-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009159 https://doaj.org/article/fd1dd6a38e1f4832803f163472df7620 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009159 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009159 https://doaj.org/article/fd1dd6a38e1f4832803f163472df7620 PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009159 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009159 2022-12-31T09:00:55Z Background Rapid diagnostic tests (RDTs) have become widely used in low-resource settings for leptospirosis diagnostic. This study aims to evaluate the diagnostic performance of the five commercially available RDTs to detect human IgM against Leptospira spp. in Thai population. Methodology/principal findings Ninety-nine serum samples from Leptospirosis suspicious patients were tested with five RDTs, including Medical Science Public Health, Leptocheck-WB, SD bioline, TRUSTline, and J.Mitra. The case definition was based on MAT, qPCR, and culture results. Diagnostic accuracy was determined based on the first day of enrollment in an overall analysis and stratified according to days post-onset of fever. The five RDTs had overall sensitivity ranging from 1.8% to 75% and specificity ranging from 52.3% to 97.7%. Leptocheck-WB had high sensitivity of 75.0%. The sensitivity of five RDTs increased on days 4-6 post-onset of fever, while the specificity of all tests remained relatively stable at different days post-onset of fever. Conclusions/significance The tested RDTs showed low sensitivity. Therefore, based on the present study, five commercially available RDTs might not be an appropriate test for acute leptospirosis screening in the Thai population. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 2 e0009159 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Janejira Dinhuzen Umaporn Limothai Sasipha Tachaboon Panadda Krairojananan Bangon Laosatiankit Sakarin Boonprasong Nuttha Lumlertgul Sadudee Peerapornratana Nattachai Srisawat A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Rapid diagnostic tests (RDTs) have become widely used in low-resource settings for leptospirosis diagnostic. This study aims to evaluate the diagnostic performance of the five commercially available RDTs to detect human IgM against Leptospira spp. in Thai population. Methodology/principal findings Ninety-nine serum samples from Leptospirosis suspicious patients were tested with five RDTs, including Medical Science Public Health, Leptocheck-WB, SD bioline, TRUSTline, and J.Mitra. The case definition was based on MAT, qPCR, and culture results. Diagnostic accuracy was determined based on the first day of enrollment in an overall analysis and stratified according to days post-onset of fever. The five RDTs had overall sensitivity ranging from 1.8% to 75% and specificity ranging from 52.3% to 97.7%. Leptocheck-WB had high sensitivity of 75.0%. The sensitivity of five RDTs increased on days 4-6 post-onset of fever, while the specificity of all tests remained relatively stable at different days post-onset of fever. Conclusions/significance The tested RDTs showed low sensitivity. Therefore, based on the present study, five commercially available RDTs might not be an appropriate test for acute leptospirosis screening in the Thai population. |
format |
Article in Journal/Newspaper |
author |
Janejira Dinhuzen Umaporn Limothai Sasipha Tachaboon Panadda Krairojananan Bangon Laosatiankit Sakarin Boonprasong Nuttha Lumlertgul Sadudee Peerapornratana Nattachai Srisawat |
author_facet |
Janejira Dinhuzen Umaporn Limothai Sasipha Tachaboon Panadda Krairojananan Bangon Laosatiankit Sakarin Boonprasong Nuttha Lumlertgul Sadudee Peerapornratana Nattachai Srisawat |
author_sort |
Janejira Dinhuzen |
title |
A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study. |
title_short |
A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study. |
title_full |
A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study. |
title_fullStr |
A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study. |
title_full_unstemmed |
A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study. |
title_sort |
prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: result from thai-lepto aki study. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009159 https://doaj.org/article/fd1dd6a38e1f4832803f163472df7620 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009159 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009159 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009159 https://doaj.org/article/fd1dd6a38e1f4832803f163472df7620 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009159 |
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PLOS Neglected Tropical Diseases |
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15 |
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e0009159 |
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