The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti
Geodia barretti is a deep-sea marine sponge common in the north Atlantic and waters outside of Norway and Sweden. The sampling and subsequent treatment as well as storage of sponges for metabolomics analyses can be performed in different ways, the most commonly used being freezing (directly upon col...
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ftdoajarticles:oai:doaj.org/article:fd0bfb65ac924a8c94ec1bb22a4ac370 2023-05-15T17:36:07+02:00 The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti Ida Erngren Eva Smit Curt Pettersson Paco Cárdenas Mikael Hedeland 2021-05-01T00:00:00Z https://doi.org/10.3389/fchem.2021.662659 https://doaj.org/article/fd0bfb65ac924a8c94ec1bb22a4ac370 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fchem.2021.662659/full https://doaj.org/toc/2296-2646 2296-2646 doi:10.3389/fchem.2021.662659 https://doaj.org/article/fd0bfb65ac924a8c94ec1bb22a4ac370 Frontiers in Chemistry, Vol 9 (2021) metabolomics liquid chromatography–mass spectrometry sponge (porifera) Geodia barretti natural products sampling Chemistry QD1-999 article 2021 ftdoajarticles https://doi.org/10.3389/fchem.2021.662659 2022-12-31T12:04:44Z Geodia barretti is a deep-sea marine sponge common in the north Atlantic and waters outside of Norway and Sweden. The sampling and subsequent treatment as well as storage of sponges for metabolomics analyses can be performed in different ways, the most commonly used being freezing (directly upon collection or later) or by storage in solvent, commonly ethanol, followed by freeze-drying. In this study we therefore investigated different sampling protocols and their effects on the detected metabolite profiles in liquid chromatography-mass spectrometry (LC-MS) using an untargeted metabolomics approach. Sponges (G. barretti) were collected outside the Swedish west coast and pieces from three sponge specimens were either flash frozen in liquid nitrogen, frozen later after the collection cruise, stored in ethanol or stored in methanol. The storage solvents as well as the actual sponge pieces were analyzed, all samples were analyzed with hydrophilic interaction liquid chromatography as well as reversed phase liquid chromatography with high resolution mass spectrometry using full-scan in positive and negative ionization mode. The data were evaluated using multivariate data analysis. The highest metabolite intensities were found in the frozen samples (flash frozen and frozen after sampling cruise) as well as in the storage solvents (methanol and ethanol). Metabolites extracted from the sponge pieces that had been stored in solvent were found in very low intensity, since the majority of metabolites were extracted to the solvents to a high degree. The exception being larger peptides and some lipids. The lowest variation between replicates were found in the flash frozen samples. In conclusion, the preferred method for sampling of sponges for metabolomics was found to be immediate freezing in liquid nitrogen. However, freezing the sponge samples after some time proved to be a reliable method as well, albeit with higher variation between the replicates. The study highlights the importance of saving ethanol extracts after ... Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Norway Frontiers in Chemistry 9 |
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metabolomics liquid chromatography–mass spectrometry sponge (porifera) Geodia barretti natural products sampling Chemistry QD1-999 |
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metabolomics liquid chromatography–mass spectrometry sponge (porifera) Geodia barretti natural products sampling Chemistry QD1-999 Ida Erngren Eva Smit Curt Pettersson Paco Cárdenas Mikael Hedeland The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti |
topic_facet |
metabolomics liquid chromatography–mass spectrometry sponge (porifera) Geodia barretti natural products sampling Chemistry QD1-999 |
description |
Geodia barretti is a deep-sea marine sponge common in the north Atlantic and waters outside of Norway and Sweden. The sampling and subsequent treatment as well as storage of sponges for metabolomics analyses can be performed in different ways, the most commonly used being freezing (directly upon collection or later) or by storage in solvent, commonly ethanol, followed by freeze-drying. In this study we therefore investigated different sampling protocols and their effects on the detected metabolite profiles in liquid chromatography-mass spectrometry (LC-MS) using an untargeted metabolomics approach. Sponges (G. barretti) were collected outside the Swedish west coast and pieces from three sponge specimens were either flash frozen in liquid nitrogen, frozen later after the collection cruise, stored in ethanol or stored in methanol. The storage solvents as well as the actual sponge pieces were analyzed, all samples were analyzed with hydrophilic interaction liquid chromatography as well as reversed phase liquid chromatography with high resolution mass spectrometry using full-scan in positive and negative ionization mode. The data were evaluated using multivariate data analysis. The highest metabolite intensities were found in the frozen samples (flash frozen and frozen after sampling cruise) as well as in the storage solvents (methanol and ethanol). Metabolites extracted from the sponge pieces that had been stored in solvent were found in very low intensity, since the majority of metabolites were extracted to the solvents to a high degree. The exception being larger peptides and some lipids. The lowest variation between replicates were found in the flash frozen samples. In conclusion, the preferred method for sampling of sponges for metabolomics was found to be immediate freezing in liquid nitrogen. However, freezing the sponge samples after some time proved to be a reliable method as well, albeit with higher variation between the replicates. The study highlights the importance of saving ethanol extracts after ... |
format |
Article in Journal/Newspaper |
author |
Ida Erngren Eva Smit Curt Pettersson Paco Cárdenas Mikael Hedeland |
author_facet |
Ida Erngren Eva Smit Curt Pettersson Paco Cárdenas Mikael Hedeland |
author_sort |
Ida Erngren |
title |
The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti |
title_short |
The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti |
title_full |
The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti |
title_fullStr |
The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti |
title_full_unstemmed |
The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti |
title_sort |
effects of sampling and storage conditions on the metabolite profile of the marine sponge geodia barretti |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fchem.2021.662659 https://doaj.org/article/fd0bfb65ac924a8c94ec1bb22a4ac370 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Frontiers in Chemistry, Vol 9 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fchem.2021.662659/full https://doaj.org/toc/2296-2646 2296-2646 doi:10.3389/fchem.2021.662659 https://doaj.org/article/fd0bfb65ac924a8c94ec1bb22a4ac370 |
op_doi |
https://doi.org/10.3389/fchem.2021.662659 |
container_title |
Frontiers in Chemistry |
container_volume |
9 |
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1766135506128601088 |