Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon (Salmo salar L.) than omega-6 and gamma tocopherol
Extended use of plant ingredients in Atlantic salmon farming has increased the need for knowledge on the effects of new nutrients and contaminants in plant based feeds on fish health and nutrient-contaminant interactions. Primary Atlantic salmon hepatocytes were exposed to a mixture of PAHs and pest...
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ftdoajarticles:oai:doaj.org/article:fcff83afc0b54186b757678a38429405 2023-05-15T15:30:50+02:00 Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon (Salmo salar L.) than omega-6 and gamma tocopherol Liv Søfteland Marc H.G. Berntssen Jennifer A. Kirwan Trond R. Størseth Mark R. Viant Bente E. Torstensen Rune Waagbø Pål A. Olsvik 2016-01-01T00:00:00Z https://doi.org/10.1016/j.toxrep.2016.01.008 https://doaj.org/article/fcff83afc0b54186b757678a38429405 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2214750016300087 https://doaj.org/toc/2214-7500 2214-7500 doi:10.1016/j.toxrep.2016.01.008 https://doaj.org/article/fcff83afc0b54186b757678a38429405 Toxicology Reports, Vol 3, Iss , Pp 211-224 (2016) Toxicology. Poisons RA1190-1270 article 2016 ftdoajarticles https://doi.org/10.1016/j.toxrep.2016.01.008 2022-12-31T03:39:06Z Extended use of plant ingredients in Atlantic salmon farming has increased the need for knowledge on the effects of new nutrients and contaminants in plant based feeds on fish health and nutrient-contaminant interactions. Primary Atlantic salmon hepatocytes were exposed to a mixture of PAHs and pesticides alone or in combination with the nutrients ARA, EPA, α-tocopherol, and γ-tocopherol according to a factorial design. Cells were screened for effects using xCELLigence cytotoxicity screening, NMR spectroscopy metabolomics, mass spectrometry lipidomics and RT-qPCR transcriptomics. The cytotoxicity results suggest that adverse effects of the contaminants can be counteracted by the nutrients. The lipidomics suggested effects on cell membrane stability and vitamin D metabolism after contaminant and fatty acid exposure. Co-exposure of the contaminants with EPA or α-tocopherol contributed to an antagonistic effect in exposed cells, with reduced effects on the VTG and FABP4 transcripts. ARA and γ-tocopherol strengthened the contaminant-induced response, ARA by contributing to an additive and synergistic induction of CYP1A, CYP3A and CPT2, and γ-tocopherol by synergistically increasing ACOX1. Individually EPA and α-tocopherol seemed more beneficial than ARA and γ-tocopherol in preventing the adverse effects induced by the contaminant mixture, though a combination of all nutrients showed the greatest ameliorating effect. Keywords: Interactions, Nutrients, PAH, Pesticides, Lipidomics, Metabolomics, RT-qPCR transcriptomics Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Toxicology Reports 3 211 224 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Toxicology. Poisons RA1190-1270 |
spellingShingle |
Toxicology. Poisons RA1190-1270 Liv Søfteland Marc H.G. Berntssen Jennifer A. Kirwan Trond R. Størseth Mark R. Viant Bente E. Torstensen Rune Waagbø Pål A. Olsvik Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon (Salmo salar L.) than omega-6 and gamma tocopherol |
topic_facet |
Toxicology. Poisons RA1190-1270 |
description |
Extended use of plant ingredients in Atlantic salmon farming has increased the need for knowledge on the effects of new nutrients and contaminants in plant based feeds on fish health and nutrient-contaminant interactions. Primary Atlantic salmon hepatocytes were exposed to a mixture of PAHs and pesticides alone or in combination with the nutrients ARA, EPA, α-tocopherol, and γ-tocopherol according to a factorial design. Cells were screened for effects using xCELLigence cytotoxicity screening, NMR spectroscopy metabolomics, mass spectrometry lipidomics and RT-qPCR transcriptomics. The cytotoxicity results suggest that adverse effects of the contaminants can be counteracted by the nutrients. The lipidomics suggested effects on cell membrane stability and vitamin D metabolism after contaminant and fatty acid exposure. Co-exposure of the contaminants with EPA or α-tocopherol contributed to an antagonistic effect in exposed cells, with reduced effects on the VTG and FABP4 transcripts. ARA and γ-tocopherol strengthened the contaminant-induced response, ARA by contributing to an additive and synergistic induction of CYP1A, CYP3A and CPT2, and γ-tocopherol by synergistically increasing ACOX1. Individually EPA and α-tocopherol seemed more beneficial than ARA and γ-tocopherol in preventing the adverse effects induced by the contaminant mixture, though a combination of all nutrients showed the greatest ameliorating effect. Keywords: Interactions, Nutrients, PAH, Pesticides, Lipidomics, Metabolomics, RT-qPCR transcriptomics |
format |
Article in Journal/Newspaper |
author |
Liv Søfteland Marc H.G. Berntssen Jennifer A. Kirwan Trond R. Størseth Mark R. Viant Bente E. Torstensen Rune Waagbø Pål A. Olsvik |
author_facet |
Liv Søfteland Marc H.G. Berntssen Jennifer A. Kirwan Trond R. Størseth Mark R. Viant Bente E. Torstensen Rune Waagbø Pål A. Olsvik |
author_sort |
Liv Søfteland |
title |
Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon (Salmo salar L.) than omega-6 and gamma tocopherol |
title_short |
Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon (Salmo salar L.) than omega-6 and gamma tocopherol |
title_full |
Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon (Salmo salar L.) than omega-6 and gamma tocopherol |
title_fullStr |
Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon (Salmo salar L.) than omega-6 and gamma tocopherol |
title_full_unstemmed |
Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon (Salmo salar L.) than omega-6 and gamma tocopherol |
title_sort |
omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for atlantic salmon (salmo salar l.) than omega-6 and gamma tocopherol |
publisher |
Elsevier |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.toxrep.2016.01.008 https://doaj.org/article/fcff83afc0b54186b757678a38429405 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Toxicology Reports, Vol 3, Iss , Pp 211-224 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2214750016300087 https://doaj.org/toc/2214-7500 2214-7500 doi:10.1016/j.toxrep.2016.01.008 https://doaj.org/article/fcff83afc0b54186b757678a38429405 |
op_doi |
https://doi.org/10.1016/j.toxrep.2016.01.008 |
container_title |
Toxicology Reports |
container_volume |
3 |
container_start_page |
211 |
op_container_end_page |
224 |
_version_ |
1766361300140556288 |