Cytotoxicity study of ethanol extract of the stem bark of asam kandis (Garcinia cowa Roxb.) on T47D breast cancer cell line

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Elidahanum Husni, Faras Nahari, Yan Wirasti, Fatma Sri Wahyuni, Dachriyanus
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2015
• Subjects: Garcinia cowa Roxb; Cytotoxicity; T47D breast cancer cell line; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/S2221-1691(15)30013-7; https://doaj.org/article/fcff11ca3e47474ebd90fc3c8b5ba887

Objective: To investigate the cytotoxic effect of ethanol extract of the stem bark of asam kandis [Garcinia cowa Roxb. (G. cowa)] on T47D breast cancer cell line. Methods: The cytotoxicity of ethanol extract was carried out against human breast cancer cell line (T47D) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay. The extract was added at various concentrations (0.1, 1, 10 and 100 μg/mL). The level of cytotoxicity was determined by calculating the level of IC50 that was based on the percentage of the cell death after 24 h treatment with the extract. Cell morphological changes were observed by using inverted microscope. Results: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that ethanol extract of G. cowa exhibited significant cytotoxic effect on T47D with IC50 value of (5.10±1.68) μg/mL. Morphological alteration of the cell lines after exposure to ethanol extract of G. cowa was observed under phase contrast microscope in a dose-dependent manner. Conclusions: The results suggest the possible use of ethanol extract of asam kandis for preparing herbal medicine for cancer-related ailments.