Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum
It has been proposed that ocean acidification (OA) will interact with other environmental factors to influence the overall impact of global change on biological systems. Accordingly we investigated the influence of nitrogen limitation and OA on the physiology of diatoms by growing the diatom Phaeoda...
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ftdoajarticles:oai:doaj.org/article:fcf570b50b2a4cf9ae79462bcdeccbeb 2023-05-15T17:49:51+02:00 Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum W. Li K. Gao J. Beardall 2015-04-01T00:00:00Z https://doi.org/10.5194/bg-12-2383-2015 https://doaj.org/article/fcf570b50b2a4cf9ae79462bcdeccbeb EN eng Copernicus Publications http://www.biogeosciences.net/12/2383/2015/bg-12-2383-2015.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-12-2383-2015 https://doaj.org/article/fcf570b50b2a4cf9ae79462bcdeccbeb Biogeosciences, Vol 12, Iss 8, Pp 2383-2393 (2015) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2015 ftdoajarticles https://doi.org/10.5194/bg-12-2383-2015 2022-12-31T03:29:44Z It has been proposed that ocean acidification (OA) will interact with other environmental factors to influence the overall impact of global change on biological systems. Accordingly we investigated the influence of nitrogen limitation and OA on the physiology of diatoms by growing the diatom Phaeodactylum tricornutum Bohlin under elevated (1000 μatm; high CO 2 – HC) or ambient (390 μatm; low CO 2 – LC) levels of CO 2 with replete (110 μmol L −1 high nitrate – HN) or reduced (10 μmol L −1 low nitrate – LN) levels of NO 3 - and subjecting the cells to solar radiation with or without UV irradiance to determine their susceptibility to UV radiation (UVR, 280–400 nm). Our results indicate that OA and UVB induced significantly higher inhibition of both the photosynthetic rate and quantum yield under LN than under HN conditions. UVA or/and UVB increased the cells' non-photochemical quenching (NPQ) regardless of the CO 2 levels. Under LN and OA conditions, activity of superoxide dismutase and catalase activities were enhanced, along with the highest sensitivity to UVB and the lowest ratio of repair to damage of PSII. HC-grown cells showed a faster recovery rate of yield under HN but not under LN conditions. We conclude therefore that nutrient limitation makes cells more prone to the deleterious effects of UV radiation and that HC conditions (ocean acidification) exacerbate this effect. The finding that nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance of the diatom P. tricornutum implies that ocean primary production and the marine biological C pump will be affected by OA under multiple stressors. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Biogeosciences 12 8 2383 2393 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 W. Li K. Gao J. Beardall Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
It has been proposed that ocean acidification (OA) will interact with other environmental factors to influence the overall impact of global change on biological systems. Accordingly we investigated the influence of nitrogen limitation and OA on the physiology of diatoms by growing the diatom Phaeodactylum tricornutum Bohlin under elevated (1000 μatm; high CO 2 – HC) or ambient (390 μatm; low CO 2 – LC) levels of CO 2 with replete (110 μmol L −1 high nitrate – HN) or reduced (10 μmol L −1 low nitrate – LN) levels of NO 3 - and subjecting the cells to solar radiation with or without UV irradiance to determine their susceptibility to UV radiation (UVR, 280–400 nm). Our results indicate that OA and UVB induced significantly higher inhibition of both the photosynthetic rate and quantum yield under LN than under HN conditions. UVA or/and UVB increased the cells' non-photochemical quenching (NPQ) regardless of the CO 2 levels. Under LN and OA conditions, activity of superoxide dismutase and catalase activities were enhanced, along with the highest sensitivity to UVB and the lowest ratio of repair to damage of PSII. HC-grown cells showed a faster recovery rate of yield under HN but not under LN conditions. We conclude therefore that nutrient limitation makes cells more prone to the deleterious effects of UV radiation and that HC conditions (ocean acidification) exacerbate this effect. The finding that nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance of the diatom P. tricornutum implies that ocean primary production and the marine biological C pump will be affected by OA under multiple stressors. |
format |
Article in Journal/Newspaper |
author |
W. Li K. Gao J. Beardall |
author_facet |
W. Li K. Gao J. Beardall |
author_sort |
W. Li |
title |
Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum |
title_short |
Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum |
title_full |
Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum |
title_fullStr |
Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum |
title_full_unstemmed |
Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum |
title_sort |
nitrate limitation and ocean acidification interact with uv-b to reduce photosynthetic performance in the diatom phaeodactylum tricornutum |
publisher |
Copernicus Publications |
publishDate |
2015 |
url |
https://doi.org/10.5194/bg-12-2383-2015 https://doaj.org/article/fcf570b50b2a4cf9ae79462bcdeccbeb |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Biogeosciences, Vol 12, Iss 8, Pp 2383-2393 (2015) |
op_relation |
http://www.biogeosciences.net/12/2383/2015/bg-12-2383-2015.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-12-2383-2015 https://doaj.org/article/fcf570b50b2a4cf9ae79462bcdeccbeb |
op_doi |
https://doi.org/10.5194/bg-12-2383-2015 |
container_title |
Biogeosciences |
container_volume |
12 |
container_issue |
8 |
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2383 |
op_container_end_page |
2393 |
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1766156358347915264 |